A Semiquantitative Rat Model for Intraperitoneal Postoperative Adhesion Formation

Abstract

A reproducible and semiquantitative rat model for the evaluation of therapeutic modalities used for the prevention of postoperative adhesion formation is designed within three experiments. In all experiments, a standard peritoneal defect was excised, sutured and adhesion formation was evaluated after 2 weeks according to the extent. Variation in extent of adhesions and type of tissue involved depended on the experimental design. While neither cecum nor colon descendens participated in the adhesions after a clamping trauma, the uterine horn was found to participate in almost all cases, especially when sutured proximally and distally to the peritoneal defect. The peritoneal defect/uterine horn model proved to be valid and reproducible and allowed a semiquantitative scoring. Additionally, the amount of blood loss as graded did not influence the presented rat adhesion model.

Efficiency of sirolimus in prevention of adhesions around vascular grafts

“…Therefore, following the involvement of neoangiogenesis in this process, the adhesive tissue causes adhesion formation. 1,[11][12][13][14] uring the adhesion formation process, fibroblast content increases in the 2 nd week, followed by inclusion of vessel structures and connective tissue elements in the adhesion . [15][16] Thus in approximately 3 weeks, the development of the adhesion becomes quite prominent.…”

Two Experimental Models for Generating Abdominal Adhesions

“…Truly objective measurements of extent require a site defined at the time of the operative preparation. One such example is the method in which a measured rectangle of peritoneum is excised and two of the edges sutured together, yielding a linear injury from which the length of adhesive attachments can be measured subsequently [10,21]. Each of the presently described models, M and T, allow for reliable quantitation of the percent area involved on a planned twodimensional flat surface, either mesh or subcutaneous tissue.…”

Preventing postoperative intraperitoneal adhesion formation with Polyactive™, a degradable copolymer acting as a barrier

“…A standard adhesion model described earlier [20] consisted of the excision of a parietal peritoneal defect of 1.5 by 1.2 cm, which was closed with three Vicryl (polyglactin 910) (Ethicon) 5-0 sutures in 2 = 2 knots. The uterine horn was clamped three times with artery forceps, for 10 s, and sutured both proximally and distally of the defect with Prolene (polypropylene) (Ethicon) 6-0 sutures [20]. All barriers were randomly assigned to the peritoneal defects and applied without suturing (Fig.…”

“…As previously described [20] the area to be scored was divided through the three vicryl sutures with which the peritoneal defect was closed, into eight areas of 12.5%. An adhesion score of 100% could thus be achieved maximally.…”

“…Polyactive TM, a copolymer based on poly(ethyleneglycol) and poly(butyleneterephthalate) with degradation characteristics that can be altered by varying the composition of the two components [15][16][17][18][19], was investigated in three different structures to study its efficacy in reducing postoperative adhesion formation. A rat paradigm was used as the standard adhesion model [20] to study a dense film, a completely porous, perforated film and a bilayered combination of a porous and dense film. Sham-operated and Interceed TMtreated animals served as controls.…”