A Sensitive and Simple Method for the Determination of Chondroitin Sulfate A with Crystal Violet by Resonance Rayleigh Scattering Technique

Abstract

Resonance Rayleigh scattering (RRS) spectra resulting from interaction between chondroitin sulfate A (CSA) and crystal violet (CV) have been investigated and applied to the determination of CSA. Though the intensity of RRS has proved very weak for CSA and CV, respectively, it can be greatly enhanced when both interact and form a supramolecular complex. A new RRS spectrum appears with a maximum scattering peak at 328 nm. In this paper, the optimum conditions of the interaction, influencing factors, and the relationship between the relative intensity of RRS (DeltaI) and the concentration of CSA have been thoroughly investigated. A new method of determination for the trace amount of CSA has been developed, which combines a simple procedure, high sensitivity, and a low detection limit of 4.8 ng/mL. It has been applied with satisfactory results to the determination of CSA in CSA injection samples and synthetic samples.

Chondroitin Sulfate Extracted from Ascidian Tunic Inhibits Phorbol Ester-Induced Expression of Inflammatory Factors VCAM-1 and COX-2 by Blocking NF-κB Activation in Mouse Skin

“…CS can be divided into several kinds such as chondroitin sulfate A (CSA), chondroitin sulfate B (CSB), and chondroitin sulfate C (CSC), etc., in which CSA and CSC are the major ingredients in almost any animal species and tissue. CSA is the alternative name for chondroitin-6-sulfate, that is, chondroitin sulfate which is sulfated on the C6 position of the GlcNAc; however, CSC is sulfated at the 4-position (24). Our analytical results showed that CS isolated from ascidian tunic was a mixture of CSA and CSC (peak 2 for CSA and peak 3 for CSC; please see figure legends for detailed information; Figure 1), and peak area analysis in HPLC demonstrated that the ratio of CSA and CSC was 46:54 (see detailed calculation method in Table 1).…”

Study on the interaction between emodin and ethyl violet by resonance Rayleigh scattering technique

“…Resonance Rayleigh scattering (RRS) and resonance nonlinear scattering (RNLS) have extensive applications [15][16][17][18][19][20][21][22] because of their sensitivity, simplicity and rapidity, and they can be achieved in a common uorescence spectrometer as new analytical methods. In recent years, these techniques have been increasingly applied to determine DNA sequence, 15 ozone, 16 DNA, 17 metal ions 18,19 and polysaccharides such as hyaluronic acid 20 and chondroitin sulfate A, 21 and pharmaceuticals.…”

“…Resonance Rayleigh scattering (RRS) and resonance nonlinear scattering (RNLS) have extensive applications [15][16][17][18][19][20][21][22] because of their sensitivity, simplicity and rapidity, and they can be achieved in a common uorescence spectrometer as new analytical methods. In recent years, these techniques have been increasingly applied to determine DNA sequence, 15 ozone, 16 DNA, 17 metal ions 18,19 and polysaccharides such as hyaluronic acid 20 and chondroitin sulfate A, 21 and pharmaceuticals. 22 In the experiment, the interaction of EMO with some basic triphenylmethane dyes, including ethyl violet (EV), crystal violet (CV), malachite green (MG), light green (LG) and methyl green (MeG), which have a similar structure and exist as cationic in aqueous solution have been investigated.…”