A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid

Goletz, Stephanie; Probst, Christian; Komorowski, Lars; Schlumberger, Wolfgang; Fechner, Kaï; Beek, Nina van; Holtsche, Maike M.; Recke, Andreas; Yancey, Kim B.; Hashimoto, Takashi; Antonicelli, Frank; Zenzo, Giovanni Di; Zillikens, Detlef; Stöcker, W.; Schmidt, Enno

doi:10.1111/bjd.17202

Cited by 61 publications

(81 citation statements)

References 43 publications

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“…Serial IgG titres correlate with disease severity . Laminin‐332 is detected in up to 20% of patients, and is associated with more severe disease and solid organ tumours in up to 30% . Further testing with ELISA or immunoblotting studies may be undertaken.…”

Section: Diagnosismentioning

confidence: 99%

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Mucous membrane pemphigoid and oral blistering diseases

Carey

Setterfield

2019

Clin Exp Dermatol

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Summary The autoimmune blistering disorders present with variable frequency in the oral cavity. Recognition of their key clinical features at presentation is important, as there are many causes of oral ulceration. Careful history‐taking, clinical examination, an understanding of pathogenesis and appropriate investigations are essential. With the exception of the rare genodermatoses that may lead to blistering and oral ulceration, the majority of patients have an acquired disorder. These include the rare autoimmune blistering diseases mucous membrane pemphigoid (MMP), pemphigus vulgaris (PV), linear IgA disease, epidermolysis bullosa acquisita and paraneoplastic pemphigus. Important clinical differential diagnoses include erythema multiforme, which may be mistaken for PV in appearance, while oral lichen planus may be indistinguishable from MMP. Angina bullosa haemorrhagica may also present with tense haemorrhagic bullae, and in the absence of diagnostic tests, requires an astute clinical diagnosis based upon the history. Newer laboratory techniques have facilitated identification of target antigens and epitopes in the autoimmune blistering diseases, particularly in MMP. Current interest is in whether these relate to clinical presentation and outcomes. There have also been recent investigations into the use of saliva as an alternative medium to serum for the diagnosis of oral vesiculobullous lesions. Assessment of disease severity and measurement of quality of life at presentation and subsequent follow‐up is paramount to interpreting therapeutic response. Furthermore, combining these scores with serological and/or salivary biomarkers is valuable in the assessment of clinical response. In this paper, we discuss MMP and its important differential diagnoses.

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Section: Diagnosismentioning

confidence: 99%

“…12,13 Laminin-332 is detected in up to 20% of patients, and is associated with more severe disease and solid organ tumours in up to 30%. [14][15][16] Further testing with ELISA or immunoblotting studies may be undertaken. BIOCHIP Mosaics TM allow polyvalent immunofluorescence tests and provide antibody profiles in a single incubation.…”

Section: Diagnosismentioning

confidence: 99%

Mucous membrane pemphigoid and oral blistering diseases

Carey

Setterfield

2019

Clin Exp Dermatol

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“…10 In a recent study by Goletz et al, IIF using recombinant laminin 332 was shown to be a valid assay for the diagnosis of laminin 332 MMP. 11 As rightly mentioned by Carey et al in their study, 1 DIF still represents the gold standard for establishing the diagnosis of both oral PV and MMP. In this regard, it is of critical importance to identify the mucosal area that is best suited for biopsy to increase the likelihood of positive DIF results being least invasive.…”

mentioning

confidence: 89%

“…reported increased sensitivity of indirect immunofluorescence (IIF) utilizing normal oral mucosa as a substrate instead of normal human skin or saline‐split human skin in 20 cases of oral MMP . In a recent study by Goletz et al ., IIF using recombinant laminin 332 was shown to be a valid assay for the diagnosis of laminin 332 MMP …”

mentioning

confidence: 99%

Improving the diagnosis of autoimmune blistering dermatoses of the oral mucosa

Maglie

Hertl

2019

Br J Dermatol

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“…This may lead to faster remission of active disease and less scarring on the mucosa. Recently, a commercial biochip assay that detects antibodies to laminin‐332 has been tested, which also detects these autoantibodies . It would be interesting to compare the sensitivity and specificity of both of these assays.…”

mentioning

confidence: 99%