A sensitive and specific HPLC method for the determination of total pentosidine concentration in plasma

Słowik-Zyłka, Dorota; Safranow, Krzysztof; Dziedziejko, Violetta; Bukowska, H.; Ciechanowski, Kazimierz; Chlubek, Dariusz

doi:10.1016/j.jbbm.2004.06.002

Cited by 18 publications

(10 citation statements)

References 40 publications

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“…2) is in accordance with corresponding data in the literature (Ex330/Em373) [26]. The models include the fluorescence wavelength at the correlation maximum between the free pentosidine concentration and the fluorescence of the spent dialysate (Fig.…”

Section: Discussionsupporting

confidence: 87%

Free Pentosidine Assessment Based on Fluorescence Measurements in Spent Dialysate

et al. 2018

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The aim of this study was to primarily explore the relationship between free pentosidine and the fluorescence properties of spent dialysate, and also to develop a model to assess the levels of free pentosidine in spent dialysate based on the fluorescence measurements. First, 40 patients (20 females and 20 males) were examined during 40 dialysis sessions. High-pressure liquid chromatography (HPLC) was used to measure the free pentosidine concentrations from the spent dialysate. The full fluorescence spectra of the spent dialysates were recorded and single- and multi-wavelength (MW) models were developed. The average free pentosidine concentrations in the spent dialysate measured by HPLC at the start and end of the dialysis session were (mean ± SD) 4.25 ± 3.11 and 0.94 ± 0.69 μg/L respectively. The removal ratios (RRs) between RR_lab and RR_MW were statistically similar (p > 0.2). The concentration of free pentosidine and the RR can therefore be estimated from the spent dialysate when utilising fluorescence measurements.

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Section: Discussionsupporting

confidence: 87%

Free Pentosidine Assessment Based on Fluorescence Measurements in Spent Dialysate

et al. 2018

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“…Pentosidine was synthesized as polypentosidine, hydrolyzed, and purified as described . Further purification on Chromabond Cross‐links SPE columns and by HPLC peak fraction collection yielded a pentosidine standard that matched published absorption and fluorescence profiles . The pentosidine standard was calibrated from its absorbance at 326 nm in 1 mM HCl .…”

Section: Methodsmentioning

confidence: 99%

“…(38) Further purification on Chromabond Cross-links SPE columns and by HPLC peak fraction collection yielded a pentosidine standard that matched published absorption and fluorescence profiles. (39) The pentosidine standard was calibrated from its absorbance at 326 nm in 1 mM HCl. (40) Standards for DHLNL and HLNL were a generous gift from Simon Robins (University of Aberdeen).…”

Section: Collagen Cross-link Quantificationmentioning

confidence: 99%

Bone Fracture Toughness and Strength Correlate With Collagen Cross-Link Maturity in a Dose-Controlled Lathyrism Mouse Model

McNerny

Gong

Morris

et al. 2014

Journal of Bone and Mineral Research

121

117

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Collagen cross-linking is altered in many diseases of bone, and enzymatic collagen cross-links are important to bone quality as evidenced by losses of strength following lysyl oxidase inhibition (lathyrism). We hypothesized that cross-links also contribute directly to bone fracture toughness. A mouse model of lathyrism using subcutaneous injection of up to 500mg/kg β-aminopropionitrile (BAPN) was developed and characterized (60 animals across 4 dosage groups). Three weeks of 150 or 350 mg/kg BAPN treatment in young growing mice significantly reduced cortical bone fracture toughness, strength, and pyridinoline cross-link content. Ratios reflecting relative cross-link maturity were positive regressors of fracture toughness (HP/[DHLNL+HLNL] r2=0.208, p<0.05; [HP+LP]/[DHNL+HLNL] r2=0.196, p<0.1), whereas quantities of mature pyridinoline cross-links were significant positive regressors of tissue strength (lysyl pyridinoline r2=0.159, p=0.014; hydroxylysyl pyridinoline r2=0.112, p<0.05). Immature and pyrrole cross-links, which were not significantly reduced by BAPN, did not correlate with mechanical properties. The effect of BAPN treatment on mechanical properties was dose specific, with the greatest impact found at the intermediate (350mg/kg) dose. Calcein labeling was used to define locations of new bone formation, allowing for the identification of regions of normally cross-linked (preexisting) and BAPN treated (newly formed, cross-link-deficient) bone. Raman spectroscopy revealed spatial differences due to relative tissue age and effects of cross-link inhibition. Newly deposited tissues had lower mineral/matrix, carbonate/phosphate and Amide I cross-link (matrix maturity) ratios compared to preexisting tissues. BAPN treatment did not affect mineral measures, but significantly increased the cross-link (matrix maturity) ratio compared to newly formed control tissue. Our study reveals that spatially localized effects of short term BAPN cross-link inhibition can alter the whole bone collagen cross-link profile to a measureable degree, and this cross-link profile correlates with bone fracture toughness and strength. Thus, cross-link profile perturbations associated with bone disease may provide insight into bone mechanical quality and fracture risk.

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“…Pentosidine and GLAP (glyceraldehyde derived pyridinium) were obtained by microwave-assisted synthesis starting from butoxy carbonyl (BOC) protected amino acids (data not shown) and characterized as described (Słowik-Zyłka et al, 2004;Usui et al, 2007).…”

Section: Synthesis Of Agesmentioning

confidence: 99%

AGEs/RAGE complex upregulates BACE1 via NF-κB pathway activation

Guglielmotto¹,

Aragno²,

Tamagno³

et al. 2012

Neurobiology of Aging

129

106

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Although the pathogenesis of sporadic Alzheimer disease (AD) is not clearly understood, it is likely dependent on several age-related factors. Diabetes is a risk factor for AD, and multiple mechanisms connecting the 2 diseases have been proposed. Hyperglycemia enhances the formation of advanced glycation end products (AGEs) that result from the auto-oxidation of glucose and fructose. The interaction of AGEs with their receptor, named RAGE, elicits the formation of reactive oxygen species that are also believed to be an early event in AD pathology. To investigate a functional link between the disorders diabetes and AD, the effect of 2 AGEs, pentosidine and glyceraldehydes-derived pyridinium (GLAP), was studied on BACE1 expression both in vivo, in streptozotocin treated rats, and in vitro in differentiated neuroblastoma cells. We showed that pentosidine and GLAP were able to upregulate BACE1 expression through their binding with RAGE and the consequent activation of NF-κB. In addition, both pentosidine and GLAP were found to be increased in the brain in sporadic AD patients. Our findings demonstrate that activation of the AGEs/RAGE axis, by upregulating the key enzyme for amyloid-β production, provides a pathologic link between diabetes mellitus and AD.

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A sensitive and specific HPLC method for the determination of total pentosidine concentration in plasma

Cited by 18 publications

References 40 publications

Free Pentosidine Assessment Based on Fluorescence Measurements in Spent Dialysate

Free Pentosidine Assessment Based on Fluorescence Measurements in Spent Dialysate

Bone Fracture Toughness and Strength Correlate With Collagen Cross-Link Maturity in a Dose-Controlled Lathyrism Mouse Model

AGEs/RAGE complex upregulates BACE1 via NF-κB pathway activation

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