A sensitive HPLC-ECD method for detecting serotonin released by RBL-2H3 cells stimulated by potential allergens

Han, Shengli; Zhang, Tao; Li, Ting; Kong, Liyun; Lv, Yanni; He, Langchong

doi:10.1039/c5ay02248g

Cited by 9 publications

(5 citation statements)

References 23 publications

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“…Our HMN‐GO.Serotonin.aptamer could accurately identify serotonin levels from 0.5–4 μM with a LOD of 0.1 μM (Figure 2F); offering an effective solution for POCT of serotonin. Moreover, the obtained LOD using our method is comparable to the LOD of current common‐practice methods for serotonin detection [47] . The HMN‐GO.Serotonin.aptamer also was highly selective towards detecting serotonin (Figure 2F, Inset).…”

Section: Resultssupporting

confidence: 57%

“…Serotonin exists in the biological range of 0.5–1.6 μM, while higher concentrations require further clinical investigation [29–31, 46] . Conventional techniques for serotonin detection mainly rely on liquid chromatography‐electrochemical detection (HPLC‐ECD), which is cumbersome and requires extensive sample pre‐treatment [47, 48] . Our HMN‐GO.Serotonin.aptamer could accurately identify serotonin levels from 0.5–4 μM with a LOD of 0.1 μM (Figure 2F); offering an effective solution for POCT of serotonin.…”

Section: Resultsmentioning

confidence: 99%

“…[29][30][31]46] Conventional techniques for serotonin detection mainly rely on liquid chromatography-electrochemical detection (HPLC-ECD), which is cumbersome and requires extensive sample pretreatment. [47,48] Our HMN-GO.Serotonin.aptamer could accurately identify serotonin levels from 0.5-4 μM with a LOD of 0.1 μM (Figure 2F); offering an effective solution for POCT of serotonin. Moreover, the obtained LOD using our method is comparable to the LOD of current commonpractice methods for serotonin detection.…”

Section: Ex Vivo Validation Of Hmn-gona Assay For Biomarker Detectionmentioning

confidence: 94%

“…Moreover, the obtained LOD using our method is comparable to the LOD of current commonpractice methods for serotonin detection. [47] The HMN-GO.Serotonin.aptamer also was highly selective towards detecting serotonin (Figure 2F, Inset).…”

Section: Ex Vivo Validation Of Hmn-gona Assay For Biomarker Detectionmentioning

confidence: 95%

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A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

et al. 2023

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Point‐of‐care testing (POCT) of clinical biomarkers is critical to health monitoring and timely treatment, yet biosensing assays capable of detecting biomarkers without the need for costly external equipment and reagents are limited. Blood‐based assays are, specifically, challenging as blood collection is invasive and follow‐upprocessing is required. Here, we report a versatile assay that employs hydrogel microneedles (HMNs) to extract interstitial fluid (ISF), in a minimally invasive manner integrated with graphene oxide‐nucleic acid (GO.NA)‐based fluorescence biosensor to sense the biomarkers of interest in situ. The HMN‐GO.NA assay is supplemented with a portable detector, enabling a complete POCT procedure. Our system could successfully measure four clinically important biomarkers (glucose, uric acid (UA), insulin, and serotonin) ex vivo, in addition, to accurately detecting glucose and UA in vivo.

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Section: Resultssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Section: Ex Vivo Validation Of Hmn-gona Assay For Biomarker Detectionmentioning

confidence: 94%

Section: Ex Vivo Validation Of Hmn-gona Assay For Biomarker Detectionmentioning

confidence: 95%

See 2 more Smart Citations

A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

et al. 2023

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“…Detection of biomarkers, which express at low concentration, is a determining factor in chronic inflammatory diseases to understand the biomolecular process of inflammation, diagnosis, prognosis, and selecting an adequate therapy. Typically, chronic inflammatory disease biomarkers are performed by conventional methods, such as enzyme-linked immunosorbent assay (ELISA) [9], high-performance liquid chromatography (HPLC) [10], polymerase chain reaction (PCR) [11], DNA sequencing technology [12], medical imaging [13], or engineered probiotic microorganisms [14]. These methods can provide very-high sensitivity and specificity, but they involve expensive and bulky instruments and complicated time-consuming operations, which limit their implementation in clinics and for practical use to the patient; therefore, it is highly desirable to develop new POC technologies for identification [1,2], with the ability to be sensitive, simple, cost effective, portable, and easy to use, which could detect infectious diseases and monitor health conditions.…”

Section: Introductionmentioning

confidence: 99%

Graphene-Based Biosensors for Molecular Chronic Inflammatory Disease Biomarker Detection

et al. 2022

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Chronic inflammatory diseases, such as cancer, diabetes mellitus, stroke, ischemic heart diseases, neurodegenerative conditions, and COVID-19 have had a high number of deaths worldwide in recent years. The accurate detection of the biomarkers for chronic inflammatory diseases can significantly improve diagnosis, as well as therapy and clinical care in patients. Graphene derivative materials (GDMs), such as pristine graphene (G), graphene oxide (GO), and reduced graphene oxide (rGO), have shown tremendous benefits for biosensing and in the development of novel biosensor devices. GDMs exhibit excellent chemical, electrical and mechanical properties, good biocompatibility, and the facility of surface modification for biomolecular recognition, opening new opportunities for simple, accurate, and sensitive detection of biomarkers. This review shows the recent advances, properties, and potentialities of GDMs for developing robust biosensors. We show the main electrochemical and optical-sensing methods based on GDMs, as well as their design and manufacture in order to integrate them into robust, wearable, remote, and smart biosensors devices. We also describe the current application of such methods and technologies for the biosensing of chronic disease biomarkers. We also describe the current application of such methods and technologies for the biosensing of chronic disease biomarkers with improved sensitivity, reaching limits of detection from the nano to atto range concentration.

show abstract

A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

et al. 2023

View full text Add to dashboard Cite

Point-of-care testing (POCT) of clinical biomarkers is critical to health monitoring and timely treatment, yet biosensing assays capable of detecting biomarkers without the need for costly external equipment and reagents are limited. Blood-based assays are, specifically, challenging as blood collection is invasive and follow-upprocessing is required. Here, we report a versatile assay that employs hydrogel microneedles (HMNs) to extract interstitial fluid (ISF), in a minimally invasive manner integrated with graphene oxide-nucleic acid (GO.NA)-based fluorescence biosensor to sense the biomarkers of interest in situ. The HMN-GO.NA assay is supplemented with a portable detector, enabling a complete POCT procedure. Our system could successfully measure four clinically important biomarkers (glucose, uric acid (UA), insulin, and serotonin) ex vivo, in addition, to accurately detecting glucose and UA in vivo.

show abstract

A sensitive HPLC-ECD method for detecting serotonin released by RBL-2H3 cells stimulated by potential allergens

Abstract: An overview of the strategy for the evaluation of potential allergens by detection of released serotonin from RBL-2H3 cells by a HPLC-ECD method.

Cited by 9 publications

References 23 publications

A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

Graphene-Based Biosensors for Molecular Chronic Inflammatory Disease Biomarker Detection

A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

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