A sensitive LC‐MS/MS method for the simultaneous determination of amoxicillin and ambroxol in human plasma with segmental monitoring

Dong, Xin; Ding, Li; Cao, Xiufeng; Jiang, Li-Yuan; Zhong, Shui-Sheng

doi:10.1002/bmc.2824

Cited by 32 publications

(22 citation statements)

References 24 publications

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“…Generally, drug analysis can't do without high performance liquid chromatography or capillary electrophoresis or LC-MS/MS or NMR et al The metabolic profiling and metabolic fingerprinting applications can be addressed as well as pharmacodynamic and pharmacokinetic issues from model organisms, animals and humans [10]. HPLC [11][12][13], LC-MS and LC-MS/MS [14] methods were applied to the drug analysis in various biological matrices for the pharmacokinetic mechanism. These methods are time-consuming and labor-intensive for the therapeutic application [15,16].…”

Section: Introductionmentioning

confidence: 99%

SERS detection of expired tetracycline hydrochloride with an optical fiber nano-probe

et al. 2015

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This paper was to develop a Raman spectra analytical method for the analysis of tetracycline hydrochloride. The surface enhanced Raman spectroscopy (SERS) were measured with the optical fiber nano-probe coated nanoparticles. Both the normal and the expired tetracycline hydrochloride were analyzed by the direct detection mode and remote detection mode. The results showed that the surface-enhanced Raman spectra could provide the chemical information for distinguishing the normal drugs from the expired ones. It demonstrated that the drug could be analyzed rapidly by SERS with optical fiber nano-probe.

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Section: Introductionmentioning

confidence: 99%

SERS detection of expired tetracycline hydrochloride with an optical fiber nano-probe

et al. 2015

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“…The AMX extraction method was modified from those previously described (Wen et al ., ; Dong et al ., ). Briefly, 200 μL of plasma sample was added with 20 μL of glacial acetic acid in a 2‐mL plastic centrifuge tube.…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of amoxicillin trihydrate in male Asian elephants (Elephas maximus) following intramuscular administration

Sinphithakkul

Klangkaew

Sanyathitiseree

et al. 2015

Vet Pharm & Therapeutics

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The purpose of this study was to investigate the pharmacokinetic characteristics of amoxicillin (AMX) trihydrate in male Asian elephants, Elephas maximus, following intramuscular administration at two dosages of 5.5 and 11 mg/kg body weight (b.w.). Blood samples were collected from 0.5 up to 72 h. The concentration of AMX in elephant plasma was measured using liquid chromatography electrospray ionization mass spectrometry. AMX was measurable up to 24 h after administration at two dosages. Peak plasma concentration (Cmax ) was 1.20 ± 0.39 μg/mL after i.m. administration at a dosage of 5.5 mg/kg b.w., whereas it was 3.40 ± 0.63 μg/mL at a dosage of 11 mg/kg b.w. A noncompartment model was developed to describe the disposition of AMX in Asian elephants. Based on the preliminary findings found in this research, the dosage of 5.5 and 11 mg/kg b.w. produced drug plasma concentrations higher than 0.25 mg/mL for 24 h after i.m. administration. Thereafter, i.m. administration with AMX at a dosage of 5.5 mg/kg b.w. appeared a more suitable dose than 11 mg/kg b.w. However, more studies are needed to determine AMX clinical effectiveness in elephants.

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“…5 Some bioanalytical methods have been reported for the determination of ambroxol by HPLC-MS/MS analysis. [6][7][8][9][10][11] In these methods, electrospray ionization (ESI) was most commonly used as an ionization method. However, in our preliminary experiments, ESI had high matrix effects for determining ambroxol in human plasma.…”

Section: Ambroxol Trans-4-(2-amino-35-dibromobenzylamino)-cyclo-mentioning

confidence: 99%

Quantitative Detection of Ambroxol in Human Plasma Using HPLC-APCI-MS/MS: Application to a Pharmacokinetic Study

et al. 2017

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In this study, a rapid and reliable high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of ambroxol in human plasma was developed and validated using palmatine as an internal standard (IS). Ambroxol and IS were extracted from 200 μL of human plasma via a simple protein precipitation preparation. Chromatographic separation was achieved on a Platisil C18 column (150 × 4.6 mm, 5 μm) using methanol-0.01% formic acid (70:30, v/v) as the mobile phase at a flow rate of 0.6 mL/min under an isocratic condition. The MS acquisition m/z 379 → 264 for ambroxol and 352 → 336 for IS was performed by atmospheric-pressure chemical ionization (APCI) mass spectrometry in selected reaction monitoring mode. The calibration curve for ambroxol was linear over the concentration range of 2.500 -180.0 ng/mL. The matrix effects of ambroxol ranged from 104.6 to 112.7%. This fully validated method was successfully applied to a pharmacokinetic study of ambroxol in humans after oral administration of ambroxol at a single dose of 75 mg.

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A sensitive LC‐MS/MS method for the simultaneous determination of amoxicillin and ambroxol in human plasma with segmental monitoring

Cited by 32 publications

References 24 publications

SERS detection of expired tetracycline hydrochloride with an optical fiber nano-probe

SERS detection of expired tetracycline hydrochloride with an optical fiber nano-probe

Pharmacokinetics of amoxicillin trihydrate in male Asian elephants (Elephas maximus) following intramuscular administration

Quantitative Detection of Ambroxol in Human Plasma Using HPLC-APCI-MS/MS: Application to a Pharmacokinetic Study

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