A sensitive LC–MS/MS method to quantify methylergonovine in human plasma and its application to a pharmacokinetic study

Gao, Yanhui; Sun, Qichao; Liu, Dongming; Ma, Bowen; Zhao, Hengli; Fang, Zengjun; Wang, Haisheng; Lou, Hongxiang

doi:10.1016/j.jchromb.2015.12.046

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…In this study, because the plasma was pretreated using the LLE method, a better extraction efficiency than simple PP was expected. The LLE method was designed using ethyl acetate as the extraction solvent with a better satisfactory extraction ability than diethyl ether [ 26 , 27 ]. The extraction efficiencies of EGCG, ECG, EGC, and EC at the low, middle, and high QC concentrations were 75.4 ± 1.55, 65.5 ± 3.66, 64.3 ± 1.84 and 61.2 ± 1.55, respectively.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Analysis of Four Catechins from Green Tea Extract in Human Plasma Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for Pharmacokinetic Studies

2018

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Green tea is consumed as a beverage worldwide and has beneficial effects, such as a lower risk of cardiovascular disease and cancer. A quantitative analysis of the beneficial components in plasma is important for understanding the potential health benefits of green tea. Four catechins—epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), epigallocatechin (EGC), and epicatechin (EC)—which account for the majority of the components of green tea, were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). In this study, a validated method was optimized to obtain the blood concentrations after the one-time ingestion of 630 mg green tea extract with digoxin and then after the ingestion of 630 mg green tea repeatedly for 15 days. The calibration curve, including the LLOQ, was constructed over 1–500 ng/mL for EGCG, ECG, and EGC and 0.1–50 ng/mL for EC. The method for inter- and intra-validation was applied, acceptable for both accuracy and precision. We successfully developed an appropriate UPLC-MS/MS method for human plasma with good reproducibility and sensitivity. Thus, this method could be applied for future preclinical and clinical studies on EGCG, ECG, EGC, and EC.

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Section: Resultsmentioning

confidence: 99%

Quantitative Analysis of Four Catechins from Green Tea Extract in Human Plasma Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for Pharmacokinetic Studies

2018

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“…All of them have been classified into two categories based on the number of analytes. Forty-nine publications involved analyzing one drug (n = 1) and the remaining 23 involved analyzing multiple drugs/metabolites (n ≥ 2) are summarized in Table 1 [4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52] and Table 2 [53,54,55,56,57,58,59,60,61,62,<...>…”

Section: Resultsmentioning

confidence: 99%

A Review on Liquid Chromatography-Tandem Mass Spectrometry Methods for Rapid Quantification of Oncology Drugs

et al. 2018

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In the last decade, the tremendous improvement in the sensitivity and also affordability of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has revolutionized its application in pharmaceutical analysis, resulting in widespread employment of LC-MS/MS in determining pharmaceutical compounds, including anticancer drugs in pharmaceutical research and also industries. Currently, LC-MS/MS has been widely used to quantify small molecule oncology drugs in various biological matrices to support preclinical and clinical pharmacokinetic studies in R&D of oncology drugs. This mini-review article will describe the state-of-the-art LC-MS/MS and its application in rapid quantification of small molecule anticancer drugs. In addition, efforts have also been made in this review to address several key aspects in the development of rapid LC-MS/MS methods, including sample preparation, chromatographic separation, and matrix effect evaluation.

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A comparative metabolomics analysis of the halophyte Suaeda salsa and Salicornia europaea

Wang

Bai

Wang

et al. 2020

Environ Geochem Health

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A sensitive LC–MS/MS method to quantify methylergonovine in human plasma and its application to a pharmacokinetic study

Cited by 7 publications

References 26 publications

Quantitative Analysis of Four Catechins from Green Tea Extract in Human Plasma Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for Pharmacokinetic Studies

Quantitative Analysis of Four Catechins from Green Tea Extract in Human Plasma Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for Pharmacokinetic Studies

A Review on Liquid Chromatography-Tandem Mass Spectrometry Methods for Rapid Quantification of Oncology Drugs

A comparative metabolomics analysis of the halophyte Suaeda salsa and Salicornia europaea

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