A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples

Liu, Yi H.; Chen, Jian; Guo, Yi; Wang, Chun M.; Liang, Xiao; Zhu, Guang

doi:10.1080/03601234.2011.559884

Cited by 13 publications

(11 citation statements)

References 22 publications

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“…5, mAb for fenpropathrin exhibited around 4%-15% cross-reactivity to cypermethrin, deltamethrin, and cyhalothrin. These minor cross-reactions seemed difficult to avoid on the basis of their similar structures, which were also observed in previous work (Wang et al, 2009;Liu et al, 2011;Chen et al, 2014). Certainly, cross-reactivity data herein may vary somewhat from values obtained in future applications, because of different assay formats, reaction systems, and so on.…”

Section: ) Chlorpyrifossupporting

confidence: 52%

Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

Liu

Lan

et al. 2016

J. Zhejiang Univ. Sci. B

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A water-soluble adjuvant named QuickAntibody (QA) was introduced into the procedure of mouse immunization for the development of hapten-specific monoclonal antibodies (mAbs), using four kinds of pesticides as model compounds. Compared with conventional Freund's adjuvants, QA treatments offered relatively low but acceptable antiserum titers after three inoculations, gave little adverse effects to the experimental animals, and were preferable in harvesting splenocytes during the steps of cell fusion. Afterwards, hybridomas from the QA group were prepared and screened by both non-competitive and competitive indirect enzyme-linked immunosorbent assays (ELISAs). The efficiency of gaining immune-positive hybridomas was satisfactory, and the resultant mAbs showed sensitivities (half maximal inhibitory concentration (IC 50)) of 0.91, 2.46, 3.72, and 6.22 ng/ml to triazophos, parathion, chlorpyrifos, and fenpropathrin, respectively. Additionally, the performance of QA adjuvant was further confirmed by acquiring a high-affinity mAb against okadaic acid (IC 50 of 0.36 ng/ml) after three immunizations. These newly developed mAbs showed similar or even better sensitivities compared with previously reported mAbs specific to the corresponding analytes. This study suggested that the easy-to-use adjuvant could be applicable to the efficient generation of highly sensitive mAbs against small compounds.

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Section: ) Chlorpyrifossupporting

confidence: 52%

Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

Liu

Lan

et al. 2016

J. Zhejiang Univ. Sci. B

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“…O ral streptococci are related to a number of health complications including dental caries, infective endocarditis, gingivitis, periodontal diseases, rheumatic diseases, and infection of the brain and liver. (1)(2)(3)(4)(5)(6)(7)(8) Most indigenous species of oral streptococci produce cell surface adhesion molecules belonging to the Ag I/II family of proteins, which interact with diverse salivary components, lysozyme, amylase, proline-rich proteins, and agglutinin. (9)(10)(11)(12)(13) Consequently, these proteins mediate the adhesion of oral streptococci to tooth surfaces and other bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…The use of monoclonal antibodies (MAbs) is a fast and selective diagnostic tool for the detection of certain cell com-ponents. (5,(19)(20)(21) Often, sensitive antibodies are critical elements in the rapid detection of organisms and a useful tool for curing disease. (22) In the present study, we investigated rapid detection of small amounts of S. mutans surface antigen I/II using a sensitive monoclonal anti-Ag I/II antibody, ckAg I/II, by ELISA.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection ofS. MutansSurface Antigen I/II Using a Sensitive Monoclonal Anti-Ag I/II Antibody by ELISA

Kim

Jeon

Shin

et al. 2013

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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The cell-surface protein antigen I/II (Ag I/II) is expressed in oral streptococci, which are known as the causative agent of a number of diseases including dental caries, endocarditis, gingivitis, and periodontal disease. Consequently, monoclonal antibodies (MAb) capable of recognizing the streptococcal Ag I/II protein could be a useful tool for the diagnosis and cure of these diseases. In this study, a previously generated monoclonal anti-Ag I/II antibody, ckAg I/II, was used to detect a small amount of Streptococcus mutans (S. mutans) surface antigen Ag I/II. The ckAg I/II was proved to be very sensitive and able to detect as little as 1 ng of recombinant Ag I/II protein within 5 min and Ag I/II in saliva within 10 min, as well as native Ag I/II in 20 μL of culture supernatant by ELISA. These results suggest that ckAg I/II can be used as a fast and efficient diagnostic tool to detect Ag I/II.

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“…Only one biochemical reaction is involved, and thus, the entire analysis time is less than 15 min, which is much faster than enzyme-linked immunosorbent assay (ELISA). 35 This process significantly improves the analysis efficiency.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The recovery rate is between 82.08 and 115.10%, and the RSD is less than 9.36% (Table S2), which shows that the MCR biosensing platform is accurate and precise. Only one biochemical reaction is involved, and thus, the entire analysis time is less than 15 min, which is much faster than enzyme-linked immunosorbent assay (ELISA) . This process significantly improves the analysis efficiency.…”

Section: Resultsmentioning

confidence: 99%

Low-Cost and Convenient Microchannel Resistance Biosensing Platform by Directly Translating Biorecognition into a Current Signal

Nie

Peng

et al. 2021

Anal. Chem.

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We report a low-cost and convenient microchannel resistance (MCR) biosensing platform that uses current signal to report biorecognition. The biorecognition behavior between targets and biometric molecules (antigens, antibodies, or oligonucleotides) immobilized on magnetic beads and polystyrene (PS) microspheres induces a quantitative change in the unreacted PS microspheres. After magnetic separation, the unreacted PS microsphere solution is passed through the microchannel, leading to an obvious blocking effect, resulting in an increase in resistance, which can in turn be measured by monitoring the electric current. Thus, the biorecognition is directly converted into a detectable current signal without any bulky instruments or additional chemical reactions. The MCR biosensing platform is cost-effective and user-friendly with high accuracy. It can be an appropriate analysis technique for point-of-care testing in resource-poor settings.

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A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples

Cited by 13 publications

References 22 publications

Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

Rapid Detection ofS. MutansSurface Antigen I/II Using a Sensitive Monoclonal Anti-Ag I/II Antibody by ELISA

Low-Cost and Convenient Microchannel Resistance Biosensing Platform by Directly Translating Biorecognition into a Current Signal

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