A Sensitive Radioimmunoassay for Human Chorionic Gonadotrophin and Luteinizing Hormone

Ce, Wilde; Ah, Orr; Kd, Bagshawe

doi:10.1677/joe.0.0370023

Cited by 53 publications

(10 citation statements)

References 18 publications

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“…Luteinizing hormone was measured by radioimmunoassay for human chorionic gonadotrophin (HCG) and LH (Wilde, Orr & Bagshawe, 1967) against a laboratory substandard of the 2nd International Reference Preparation of human menopausal gonadotrophin (2nd I.R.P. HMG) and expressed as i.u.…”

Section: Methodsmentioning

confidence: 99%

Luteinizing Hormone Levels in Plasma and Urine in Women During Normal Menstrual Cycles and in Women Taking Combined Contraceptives or Chlormadinone Acetate

Orr¹,

Elstein²

1969

Journal of Endocrinology

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Radioimmunoassay measurements of luteinizing hormone (LH) levels were made in urine and plasma throughout normal menstrual cycles and cycles when either a combined oral contraceptive or low dosage continuous chlormadinone acetate were being taken by healthy young women. The typical mid-cycle ovulatory increase of LH was suppressed in the cycles when the combined contraceptive preparations were taken but was present in the majority of cycles during the administration of continuous chlormadinone acetate.

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Section: Methodsmentioning

confidence: 99%

Luteinizing Hormone Levels in Plasma and Urine in Women During Normal Menstrual Cycles and in Women Taking Combined Contraceptives or Chlormadinone Acetate

Orr¹,

Elstein²

1969

Journal of Endocrinology

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“…Although immunologie reactions obey the law of mass action and are reversible, the association constants are sufficiently high that it is unlikely that minor interfering factors can have a significant effect once the formation of the antigen-antibody aggregates is well underway. Although this approach has been used (Wilde et al 1967;Jorgensen 1969), surprisingly it has not been studied extensively. Part of the reason undoubtedly lies in the need to know the number of tubes desired in an assay at least one day in advance of actually adding the sample.…”

Section: Use Of Pre-precipitated Antibodymentioning

confidence: 99%

Radioimmunoassays Employing Double Antibody Techniques

Midgley

Rebar

Niswender

1969

Acta Endocrinologica

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Double antibody methods are applicable to all radioimmunoassay systems. At least three approaches exist. The first, in which immunoprecipitating serum is used to precipitate soluble antigen-antibody complexes, is the most commonly used. Since the effects of interfering factors, especially those present in serum, become minimal as equilibrium is approached, care must be taken to insure that the period of incubation is of sufficient length for the immunoprecipitation reaction to reach equilibrium. In general, all efforts should focus on achieving reaction conditions which maximize stability and optimize immunoprecipitation. A second approach involves use of the first antiserum in an insolubilized form after prior precipitation with the immunoprecipitating antiserum. This approach is attractive since most non specific factors cannot interfere with the immunoprecipitation step to a significant extent once the formation of antigen-antibody aggregates is well underway. The third approach, in which immunoprecipitating antiserum is conjugated to a solid matrix, in principle represents a more ideal method of separating bound hormone from free. If this approach is ever to be utilized, a method of conjugating antiserum to a suitable matrix such that the antibody retains most of its immunoreactivity must be devised.

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“…Fasting plasma glucagon (Bloom, 1972) was 140 pg/ml at 37 weeks, which is in the normal nonpregnant range. Plasma chorionic gonadotrophin (Wylde et al, 1967) was 10 8 IU/ml at 37 weeks.…”

Section: Casementioning

confidence: 94%

“…Fasting plasma glucagon (Bloom, 1972) was 140 pg/ml at 37 weeks, which is in the normal nonpregnant range. Plasma chorionic gonadotrophin (Wylde et al, 1967) The association of such low serum insulin values with virtually normal glucose tolerance indicates a degree of insulin sensitivity remarkable in the pregnant diabetic, in whom insulin antagonism usually develops (Kalkhoff et al, 1964).…”

Section: Casementioning

confidence: 99%

Remission of Diabetes Mellitus during Pregnancy

Sheldon¹,

Coleman²

1974

BMJ

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55with acute myeloid leukaemia is of great clinical importance. Water intoxication may produce confusion (Kaye, 1966) and disorientation (Weissman et al., 1971)-symptoms which can readily be confused with the terminal state of acute leukaemia. Intravenous infusion can be hazardous inthese patients. Attention to the underlying electrolyte imbalance will lead to the correct diagnosis, which is reversible by water restriction.We appreciate the kind co-operation of Dr.

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A Sensitive Radioimmunoassay for Human Chorionic Gonadotrophin and Luteinizing Hormone

Cited by 53 publications

References 18 publications

Luteinizing Hormone Levels in Plasma and Urine in Women During Normal Menstrual Cycles and in Women Taking Combined Contraceptives or Chlormadinone Acetate

Luteinizing Hormone Levels in Plasma and Urine in Women During Normal Menstrual Cycles and in Women Taking Combined Contraceptives or Chlormadinone Acetate

Radioimmunoassays Employing Double Antibody Techniques

Remission of Diabetes Mellitus during Pregnancy

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