A sequential utilization of the UV-A (365 nm) fluence rate for disinfection of water, contaminated with Legionella pneumophila and Legionella dumoffii

Allahyari, Elahe; Carraturo, Federica; Risi, Arianna De; Nappo, Antonio; Morelli, Michela; Cajora, Alessia; Guida, Marco

doi:10.1016/j.envpol.2022.119224

Cited by 7 publications

(10 citation statements)

References 46 publications

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“…pneumophila using only UVA wavelengths. As previously mentioned, it is still debated whether wavelengths higher than 315 nm induce photodamage to genomic DNA and repair proteins . These mechanisms are not apparent in this study, as all the quantification methods showed similar results.…”

Section: Resultsmentioning

confidence: 60%

“…These previous studies used 254 nm LP or MP UV lamps. Therefore, it is essential to investigate the effects of higher wavelengths, such as 280 and 365 nm, using UV LED devices to determine if they may or may not prevent or reduce photoreactivation by causing damage to nucleic acids and repair enzymes . Photoreactivation studies were not in the scope of this study, but the authors agree that it is important to address them for future research.…”

Section: Resultsmentioning

confidence: 99%

“…As previously mentioned, it is still debated whether wavelengths higher than 315 nm induce photodamage to genomic DNA and repair proteins. 26 These mechanisms are not apparent in this study, as all the quantification methods showed similar results. It is hypothesized that the mechanism for 365 nm damage is captured within the range of fluences examined in this study (20−200 mJ/cm 2 ) but may not be captured if higher fluences were used.…”

Section: Inactivation Of P Fluorescensmentioning

confidence: 97%

“…23,24 Additionally, debates persist regarding wavelengths >315 nm (UVA), which have the potential to inflict irreparable damage to DNA, proteins, and repair enzymes, thereby impeding photoreactivation mechanisms. 25,26 These emerging findings underscore the necessity of exploring alternative wavelengths beyond the conventional 254 nm. 20,27,28 There is limited literature examining the use of UV LEDs for inactivating planktonic and biofilm-associated pathogens.…”

Section: Introductionmentioning

confidence: 99%

“…The current standard wavelength for UV treatment is 254 nm. However, new information indicates that wavelengths closer to 280 nm (UVC) hold promise as a reliable and energy-efficient option for water disinfection. , Additionally, debates persist regarding wavelengths >315 nm (UVA), which have the potential to inflict irreparable damage to DNA, proteins, and repair enzymes, thereby impeding photoreactivation mechanisms. , These emerging findings underscore the necessity of exploring alternative wavelengths beyond the conventional 254 nm. ,, …”

Section: Introductionmentioning

confidence: 99%

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Comparison of Legionella pneumophila and Pseudomonas fluorescens Quantification Methods for Assessing UV LED Disinfection

Lara de Larrea,

MacIsaac,

Rauch

et al. 2023

ACS EST Water

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This study assesses the efficacy of ultraviolet light-emitting diodes (UV LEDs) for deactivating Legionella pneumophila (pure culture) and Pseudomonas fluorescens (pure culture and biofilms) on relevant drinking water distribution system surfaces (cast iron and stainless steel). UV LED treatment at 280 nm demonstrated superior performance compared to that at 365 nm, achieving a 4.8 log reduction value (LRV) for P. fluorescens pure cultures and, for biofilms, 4.02 LRV for stainless steel and 2.96 LRV for cast iron at 280 nm. Conversely, the results were less effective at 365 nm, with suspected photolytic reactions on cast iron. Quantification of L. pneumophila yielded varying results: 4 LRV using standard plate counts, 1.8 LRV with Legiolert, and 1 LRV with quantitative polymerase chain reaction at 280 nm, while the results were less than 1.5 LRV at 365 nm. This study provides insights into managing opportunistic pathogens and biofilms, emphasizing the need for improved quantification tools to better assess treatment efficacy.

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Section: Resultsmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 99%

Section: Inactivation Of P Fluorescensmentioning

confidence: 97%