2004
DOI: 10.1021/bi0486334
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A Series of Related Nucleotide Analogues that Aids Optimization of Fluorescence Signals in Probing the Mechanism of P-Loop ATPases, Such as Actomyosin

Abstract: We have synthesized a set of ATP and ADP analogues that have a fluorophore linked to the nucleotide via the 3'-position of the ribose moiety. Combinations of three different coumarins are each attached via different length linkers. A linker based on propylenediamine increases the separation between the nucleotide and fluorophore relative to that of the previously reported ethylenediamine-linked coumarin nucleotides [Webb, M. R., and Corrie, J. E. T. (2001) Biophys. J. 81, 1562-1569]. A synthesis of 3'-amino-3'… Show more

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Cited by 27 publications
(28 citation statements)
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“…[39] In aqueous medium, DEAC-containing acylcholines exhibit fluorescence quantum yields of 0.026 (Table 2) with fluorescence emission maxima at 478 nm, comparable with other DEAC-containing ligands. [37] In protic organic solvents (ethanol, butan-1-ol) the fluorescence quantum yields of DEAC-containing acylcholines are significantly increased and the fluorescence emission maxima are blue-shifted (data not shown), an observation consistent with results obtained with other 7-(dialkylamino)coumarin dyes. [39] These results suggest that DEAC-G-C6-Ch occupies a more hydrophobic environment in the receptor bound state.…”
Section: Deac-g-c6-ch Binds To Desensitized Torpedo Nachr With Nanomosupporting
confidence: 79%
See 1 more Smart Citation
“…[39] In aqueous medium, DEAC-containing acylcholines exhibit fluorescence quantum yields of 0.026 (Table 2) with fluorescence emission maxima at 478 nm, comparable with other DEAC-containing ligands. [37] In protic organic solvents (ethanol, butan-1-ol) the fluorescence quantum yields of DEAC-containing acylcholines are significantly increased and the fluorescence emission maxima are blue-shifted (data not shown), an observation consistent with results obtained with other 7-(dialkylamino)coumarin dyes. [39] These results suggest that DEAC-G-C6-Ch occupies a more hydrophobic environment in the receptor bound state.…”
Section: Deac-g-c6-ch Binds To Desensitized Torpedo Nachr With Nanomosupporting
confidence: 79%
“…[11] DEAC-G-C6-Ch binding to Torpedo nAChR induced a strong increase in fluorescence intensity DEAC is well known as an environment-sensitive fluorophore and has been used in ligand-receptor binding studies. [37,38] In those studies, binding of DEAC-containing ligands induced strong enhancements of the fluorescence intensities due to specific ligand-receptor interactions. As shown in detailed photophysical studies, an increase in fluorescence intensity and a blue shift of the fluorescence maximum can be attributed to changes in the dipole moment and in the electronic structure, due to an increase in the solvent hydrophobicity of the surrounding medium.…”
Section: Deac-g-c6-ch Binds To Desensitized Torpedo Nachr With Nanomomentioning
confidence: 99%
“…N-Methylanthraniloyl derivatives of the 2Ј-deoxynulceotides mdADP and mdATP were synthesized and purified according to the method of Hiratsuka (16). Deac-aminoADP and deacaminoATP were synthesized by the method of Webb et al (17 ) were followed in a Beckman DU640 spectrophotometer. The ATPase activity of blanks containing actin but no myosin was subtracted from the actomyosin data.…”
Section: Methodsmentioning
confidence: 99%
“…N-Methylanthraniloyl derivatives of 2Ј-deoxyADP (mdADP) and ATP (mdATP) were synthesized and purified according to the method of Hiratsuka (20). Deac-aminoATP and deac-amino-ADP were synthesized by the method of Webb et al (21). Deacaminonucleotide concentrations were based upon molar extinction coefficients of the deac-aminoATP and deac-amino-ADP, ⑀ 429 ϭ 46,800 M Ϫ1 cm Ϫ1 .…”
Section: Methodsmentioning
confidence: 99%