A serine proteinase inhibitor isolated from Tamarindus indica seeds and its effects on the release of human neutrophil elastase

Fook, Jacy M.S.L.L.; Macedo, Leonardo Lima Pepino; Moura, Gioconda Emanuella Diniz de Dantas; Teixeira, Fabiano M.; Oliveira, Adeliana S.; Queiroz, Alexandre F.S.; Sales, Maurı́cio P.

doi:10.1016/j.lfs.2004.10.053

Cited by 42 publications

(31 citation statements)

References 46 publications

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“…Till date trypsin inhibitors have been purified and characterized from a variety of plant sources (Macedo et al2000;Oliviera et al 2007;Kansal et al 2008;Rai et al 2008). The purified inhibitor protein was found to consist of a single polypeptide chain of 12,303 daltons as revealed by Native-PAGE and SDS-PAGE, which is consistent with the molecular masses of other trypsin inhibitors (Mizuta and Ventura 1976;Fook et al 2005;Sivakumar et al 2005). On the contrary trypsin inhibitor purified from A. kalkora seeds showed the presence of two disulfide linked polypeptide chains and a high molecular mass (Zhou et al 2008).…”

Section: Discussionsupporting

confidence: 60%

Purification, characterization and evaluation of insecticidal activity of trypsin inhibitor from Albizia lebbeck seeds

Sharma

Nath

Kumari

et al. 2012

Journal of Forestry Research

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A Bowman-Birk inhibitor with activity against gut proteases of Helicoverpa armigera was extracted in 0.1 M sodium phosphate buffer from defatted seed flour of Albizia lebbeck. It was purified to 29.62 folds with 51.43% recovery using ammonium sulfate precipitation, gel filtration chromatography on Sephadex G-100 column and ion exchange chromatography on DEAE-Sephadex A 50 . The purified protein had a molecular weight of 12,303 daltons as determined by SDS-PAGE.It was found to be heat stable up to 60°C and had two pH optima of 7.5 and 9.0. The inhibitor exhibited non-competitive pattern of inhibition with a low K i value of 0.2 µM. The inhibitor was found to be susceptible to varying concentrations of reducing agents like DTT and 2-mercaptoethanol, thereby indicating the role of disulphide bridges in maintaining its three dimensional structure and stability. The purified inhibitor caused mortality and suppressed larval growth of Pieris brassicae larvae. It was also found to be effective against gut trypsin extracted from Spodoptera littoralis. The sequence of the genes encoding for such inhibitors can be determined and the genes expressing protease inhibitors can be used in vegetable crops to confer resistance against insect pests and other plant pathogens.

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Section: Discussionsupporting

confidence: 60%

Purification, characterization and evaluation of insecticidal activity of trypsin inhibitor from Albizia lebbeck seeds

Sharma

Nath

Kumari

et al. 2012

Journal of Forestry Research

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“…It is clear that the inhibitory actions of the hawthorn extracts on prolyl endopeptidase are not attributable to these low molecular weight phenolic compounds. For another common protease, elastase, which is distributed in various tissues, such as the pancreas, lung, and neutrophils, 42) the results shown in Table 2 indicate that hawthorn extracts do not have significant inhibitory action. This indicates that the inhibitory action of the hawthorn extracts on prolyl endopeptidase is selective.…”

Section: Protease Inhibitory Activitiesmentioning

confidence: 98%

Polyphenolic Content and Physiological Activities of Chinese Hawthorn Extracts

Cui

Nakamura

Tian

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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Hawthorn polyphenol (HP) was prepared by ethyl acetate treatment of the ethanol extract (HE) of Chinese hawthorn fruit. The concentrations of 15 polyphenols in the HP, HE, extraction residue (HJ), and a hawthorn leaf extract (HF) were determined by HPLC. For HP, the total content of the 15 polyphenols was 21.4%, comprised of 19.7% of procyanidins, 1.21% of chlorogenic acid, and 0.48% of flavonoids, compared to 2.55% for the HE. The yields of procyanidin monomer, dimer, trimer, tetramer, and pentamer were 50.5%, 30.3%, 23.0%, 14.6%, and 12.5% respectively, and the mean degree of polymerization was reduced to 1.39 (HP) from 1.65 (HE). Seven different physiological actions of the four extracts were investigated. The HP showed strong O 2 À and OH scavenging capacities (IC 50 values of 6.3 g/ml and 1.1 g/ml respectively), as well as selective prolyl endopeptidase inhibition (IC 50 of 60 g/ml). The active constituents appeared to be procyanidins.Key words: Crataegus pinnatifida; HPLC; procyanidin; antioxidant; free radicalsIn Europe, standardized extracts of the leaves and flowers of Crataegus oxyacantha and Crataegus monogyna are used in the therapy for moderate (NYHA II) congestive heart failure. 1,2) These extracts exert several pharmacological and clinical effects, such as antiischemia/reperfusion-injury, anti-arrhythmic, hypolipidemic, and hypotensive effects. 1,2) Flavonoids and procyanidins are considered to be the two main active constituents. 2) Species of Chinese hawthorn, particularly Crataegus pinnatifida and Crataegus pinnatifida Bge. var. major NE Br., have also been used as herbal medicines. 2,3) Studies indicate that Chinese hawthorn extracts have beneficial effects, such as antioxidant, 4,5) anti-inflammatory, 6) and hypolipidemic effects, 7) as well as protective effects on the brain and vascular endothelium 8,9) and endothelium-dependent relaxation. 10) Recently, we analyzed 37 major cultivars of Chinese hawthorn using high performance liquid chromatography (HPLC), and found that procyanidins, which were present in high concentrations, were the main phenolic active constituents in these fruits. 11) In general, the hawthorn found in China is an edible fruit that is popular for its special acidic taste. It is bigger than its European counterpart. Since the content of organic acid in Chinese hawthorn fruits can be up to 5% (fresh weight), they are used to produce various foods, such as jams, jellies, juices, tinned foods, wines, and various sweet foods. However, the high sugar content of these fruits counteracts the benefits of polyphenols for consumers of advanced age or for those who suffer from hypertension, cardiovascular disease, or diabetes. Hence, it is desirable to separate the polyphenol and sugar components. Previously, hawthorn extract (HE) for medical applications were ethanol-extracted from the dried fruit of the Chinese hawthorn. 12) These crude extracts contain many undesirable substances, such as carbohydrates, organic acids, and moisture, which not only reduce the concentration of acti...

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“…Water extract of tamarind seed has been shown to reduce blood sugar level in Streptozotocin-induced diabetic male rats (Maiti et al 2004). Tamarind seeds are known to have high inhibitory activity against human neutrophil elastase (Fook et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activities of tamarind (Tamarindus Indica) seed coat extracts using in vitro and in vivo models

2014

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Tamarindus indica seed coat was extracted with methanol, acetone and water and screened for DPPH radical scavenging activities. Methanol extract showed higher activity than other extracts. Treatment of albino rats (Wistar strain) with CCl 4 at 1.25 mL/kg of body weight decreased superoxide dismutase (55 %), catalase (73 %) and peroxidase (78 %), while lipid peroxidation increased nearly 2.5 fold in liver. Pretreatment of rats with methanol extract of T. Indica seed coat (TSCE) at 50 mg/kg (as tannic acid equivalents) followed by CCl 4 treatment, caused restoration of superoxide dismutase, catalase and lipid peroxidation to values close to control while peroxidase was restored to 67 % of the control. Histopathological studies of liver of different groups supported the protective effects of TSCE by restoring the hepatic architecture. These studies could be further extended to exploit its possible application for the preservation of food products as well as a health supplement and neutraceutical.

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A serine proteinase inhibitor isolated from Tamarindus indica seeds and its effects on the release of human neutrophil elastase

Cited by 42 publications

References 46 publications

Purification, characterization and evaluation of insecticidal activity of trypsin inhibitor from Albizia lebbeck seeds

Purification, characterization and evaluation of insecticidal activity of trypsin inhibitor from Albizia lebbeck seeds

Polyphenolic Content and Physiological Activities of Chinese Hawthorn Extracts

Antioxidant activities of tamarind (Tamarindus Indica) seed coat extracts using in vitro and in vivo models

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