A serological survey of severe acute respiratory syndrome coronavirus 2 in dogs in Wuhan

Zhao, Ya; Yang, Yong; Gao, Jindong; Huang, Kun; Hu, Changmin; Hui, Xianfeng; He, Xinglin; Li, Chengfei; Gong, Wenxiao; Lv, Changjie; Zhang, Yufei; Chen, Huanchun; Zou, Zhong; Zhang, Qiang; Jin, Meilin

doi:10.1111/tbed.14024

Cited by 34 publications

(44 citation statements)

References 24 publications

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“…In Italy, the antibody neutralization assay was used to monitor SARS-CoV-2 infection in 451 dogs during the pandemic, and 15 (3.3%) dogs were found seropositive [ 20 ]. In Wuhan city (China), 16 (1.7%) positive dogs were detected among the 946 using a newly developed double-antigen sandwich ELISA assay [ 21 ]. In Croatia, an investigation revealed that 7.6% of dogs (13/172) were positive by ELISA assay [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

SARS-CoV-2 antibodies seroprevalence in dogs from France using ELISA and an automated western blotting assay

et al. 2021

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Dogs are occasionally susceptible to SARS-CoV-2, developing few or no clinical signs. Epidemiological surveillance of SARS-CoV-2 in dogs requires testing to distinguish it from other canine coronaviruses. In the last year, significant advances have been made in the diagnosis of SARS-CoV-2, allowing its surveillance in both human and animal populations. Here, using ELISA and automated western blotting (AWB) assays, we performed a longitudinal study on 809 apparently healthy dogs from different regions of France to investigate anti-SARS-CoV-2 antibodies. There were three main groups: (i) 356 dogs sampled once before the pandemic, (ii) 235 dogs sampled once during the pandemic, and (iii) 218 dogs, including 82 dogs sampled twice (before and during the pandemic), 125 dogs sampled twice during the pandemic and 11 dogs sampled three times (once before and twice during the pandemic). Using ELISA, seroprevalence was significantly higher during the pandemic [5.5% (25/453)] than during the pre-pandemic period [1.1% (5/449)]. Among the 218 dogs sampled twice, at least 8 ELISA-seroconversions were observed. ELISA positive pre-pandemic sera were not confirmed in serial tests by AWB, indicating possible ELISA cross-reactivity, probably with other canine coronaviruses. A significant difference was observed between these two serological tests (Q = 88, p = 0.008). A clear correlation was observed between SARS-CoV-2 seroprevalence in dogs and the incidence of SARS-CoV-2 infection in human population from the same area. AWB could be used as a second line assay to confirm the doubtful and discrepant ELISA results in dogs. Our results confirm the previous experimental models regarding the susceptibility of dogs to SARS-CoV-2, suggesting that viral transmission from and between dogs is weak or absent. However, the new variants with multiple mutations could adapt to dogs; this hypothesis cannot be ruled out in the absence of genomic data on SARS-CoV-2 from dogs.

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Section: Discussionmentioning

confidence: 99%

SARS-CoV-2 antibodies seroprevalence in dogs from France using ELISA and an automated western blotting assay

et al. 2021

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“…Given the evidence for susceptibility of wild, domestic and farmed animals to infection by SARS-CoV-2 and the public health risks associated with these infections, there is an urgent need for suitable tools for the sero surveillance and monitoring of different animal species. To date, there are just a few serological testing methods for SARS-CoV-2 in animals based on either ELISA, PRNT, VNT or an indirect immunofluorescence assay [ 26 , 27 , 28 , 29 , 30 ]. One study has employed LIPS using both the S and N target to screen a small number of dogs ( n = 12) and cats ( n = 9) that had repeated contacts with SARS-CoV-2-infected humans, but the authors did not detect any anti-SARS-CoV-2 antibodies in the sera of these animals [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Serological Detection of SARS-CoV-2 Antibodies in Naturally-Infected Mink and Other Experimentally-Infected Animals

Berguido

Burbelo

Bortolami

et al. 2021

Viruses

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The recent emergence of SARS-CoV-2 in humans from a yet unidentified animal reservoir and the capacity of the virus to naturally infect pets, farmed animals and potentially wild animals has highlighted the need for serological surveillance tools. In this study, the luciferase immunoprecipitation systems (LIPS), employing the spike (S) and nucleocapsid proteins (N) of SARS-CoV-2, was used to examine the suitability of the assay for antibody detection in different animal species. Sera from SARS-CoV-2 naturally-infected mink (n = 77), SARS-CoV-2 experimentally-infected ferrets, fruit bats and hamsters and a rabbit vaccinated with a purified spike protein were examined for antibodies using the SARS-CoV-2 N and/or S proteins. From comparison with the known neutralization status of the serum samples, statistical analyses including calculation of the Spearman rank-order-correlation coefficient and Cohen’s kappa agreement were used to interpret the antibody results and diagnostic performance. The LIPS immunoassay robustly detected the presence of viral antibodies in naturally infected SARS-CoV-2 mink, experimentally infected ferrets, fruit bats and hamsters as well as in an immunized rabbit. For the SARS-CoV-2-LIPS-S assay, there was a good level of discrimination between the positive and negative samples for each of the five species tested with 100% agreement with the virus neutralization results. In contrast, the SARS-CoV-2-LIPS-N assay did not consistently differentiate between SARS-CoV-2 positive and negative sera. This study demonstrates the suitability of the SARS-CoV-2-LIPS-S assay for the sero-surveillance of SARS-CoV-2 infection in a range of animal species.

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“…Two dogs from Hong Kong tested positive for SARS-CoV-2; a 17-year-old dog with comorbidities tested positive over a 13-day period, and a 2-year-old dog that tested positive and showed shedding of viable virus in oral and nasal swabs [ 68 ]. Multiple surveillance studies also demonstrated a low prevalence of seropositive dogs in regions with active SARS-CoV-2 outbreaks [ 49 , 50 , 56 , 57 , 59 , 61 , 62 , 69 , 70 ]. However, other surveillance studies failed to show any transmission or seroconversion in dogs, including the study involving SARS-CoV-2-infected students in prolonged contact with several pet dogs [ 44 , 154 ].…”

Section: Main Textmentioning

confidence: 99%

Natural and Experimental SARS-CoV-2 Infection in Domestic and Wild Animals

Meekins

Gaudreault

Richt

2021

Viruses

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SARS-CoV-2 is the etiological agent responsible for the ongoing COVID-19 pandemic, which continues to spread with devastating effects on global health and socioeconomics. The susceptibility of domestic and wild animal species to infection is a critical facet of SARS-CoV-2 ecology, since reverse zoonotic spillover events resulting in SARS-CoV-2 outbreaks in animal populations could result in the establishment of new virus reservoirs. Adaptive mutations in the virus to new animal species could also complicate ongoing mitigation strategies to combat SARS-CoV-2. In addition, animal species susceptible to SARS-CoV-2 infection are essential as standardized preclinical models for the development and efficacy testing of vaccines and therapeutics. In this review, we summarize the current findings regarding the susceptibility of different domestic and wild animal species to experimental SARS-CoV-2 infection and provide detailed descriptions of the clinical disease and transmissibility in these animals. In addition, we outline the documented natural infections in animals that have occurred at the human–animal interface. A comprehensive understanding of animal susceptibility to SARS-CoV-2 is crucial to inform public health, veterinary, and agricultural systems, and to guide environmental policies.

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A serological survey of severe acute respiratory syndrome coronavirus 2 in dogs in Wuhan

Cited by 34 publications

References 24 publications

SARS-CoV-2 antibodies seroprevalence in dogs from France using ELISA and an automated western blotting assay

SARS-CoV-2 antibodies seroprevalence in dogs from France using ELISA and an automated western blotting assay

Serological Detection of SARS-CoV-2 Antibodies in Naturally-Infected Mink and Other Experimentally-Infected Animals

Natural and Experimental SARS-CoV-2 Infection in Domestic and Wild Animals

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