A serum factor with potential as a tumour marker in malignant lymphoma

Abstract

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Identification of PEG-precipitable serum factor associated with malignant lymphoma as C-reactive protein

“…We conclude that the serum factor in malignant lymphoma, described in a previous publication (Begent et al, 1980), is CRP and that its detection in material precipitated by PEG was a consequence of both cation-dependent and -independent complexing of CRP.…”

“…A recent report described a serum factor with potential as a monitor of malignant lymphoma (Begent et al, 1980). The protein could be precipitated from serum by low concentrations of polyethylene glycol 6000 (PEG) and had a mol.…”

“…SDS-PAGE and PEG precipitation were performed as described previously (Begent et al, 1980). PEG precipitates were solubilised in SDS sample buffer (2% SDS, 10% glycerol in 0.1 M Tris, pH 6.8) for SDS-PAGE or barbital buffer, 0.075 m, pH 8.6 containing 0.01 M EDTA for quantitation of CRP by rocket immunoelectrophoresis (Laurell, 1972).…”