A set of microsatellite markers for population genetics of leopard cat (Prionailurus bengalensis) and cross-species amplification in other felids

Eo, Soo Hyung; Ko, Byung June; Lee, Byeong-Ju; Seomun, Hong; Kim, Soon-Ok; Kim, Mun-Jeong; Kim, Jeong-Ho; An, Junghwa

doi:10.1016/j.bse.2016.04.005

Cited by 9 publications

(16 citation statements)

References 17 publications

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“…Primers were re-designed to amplify repeated regions according to the reference sequences (Eo et al, 2016) using Primer 3, with a product length of 90–220 bp for non-invasive samples (Table 1). These microsatellite markers were synthesized with fluorescent labels and PCR reactions were performed by multi-plex and single-plex PCR (Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…Sequences of M13F and Tail C are 5′-GTTGTAAAACGACGGCCAGT-3′ and 5′-CAGGACCAGGCTACCGTG-3′, respectively. Reverse primers are labeled at the 5′ end with pigtail sequences (5′-GTTTCTT-3′).aGenbank accession number from Eo et al (2016).…”

Section: Methodsmentioning

confidence: 99%

“…To compare the genetic structure and diversity of the Tsushima population with other regional populations, data from a previous dataset from the Korean population were used ( n = 30) (Eo et al, 2016). The coincidence between the genotypes reported in the previous study (Eo et al, 2016) and that observed in this study was confirmed by sequencing PCR products at 12 loci in two Tsushima leopard cats and comparing the number of repetitions of microsatellite motifs between the two studies. Alleles with the same number of repetitions of microsatellite motifs in both studies were assumed to be the same.…”

Section: Methodsmentioning

confidence: 99%

“…2 (Do et al, 2014). In the Korean population, three variables ( Na , He and Ho ) were cited data from the previous study (Eo et al, 2016), while the other variables were calculated as part of this study. F IS (population differentiation between individuals among sampled populations), F IT (population differentiation among individuals), and F ST (population differentiation between populations) were calculated using GENEPOP ver.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, the use of next-generation sequencing techniques for the identification of microsatellite markers has been found to reduce both time and costs, and these techniques can serve as a powerful tool for genetic studies in non-model and endangered species. Eo et al (2016) have identified 12 new microsatellite markers based on genomic information of the Korean Amur leopard cat and have tested these markers in several related species. Using molecular genetics, the divergence between the Tsushima and continental populations was estimated to have occurred approximately 100,000 years ago (Masuda & Yoshida, 1995).…”

Section: Introductionmentioning

confidence: 99%

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The Tsushima leopard cat exhibits extremely low genetic diversity compared with the Korean Amur leopard cat: Implications for conservation

Itô

Inoue‐Murayama

2019

PeerJ

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We examined genetic diversity of the wild Tsushima leopard cat—a regional population of the Amur leopard cat—using microsatellite markers. In addition, we compared genetic diversity of the Tsushima leopard cat with that of the Korean population of Amur leopard cat. Although bias should be considered when applying cross-species amplification, the Tsushima leopard cat showed a lower index of molecular genetic diversity than did the Korean population. These results were consistent with those obtained using other genetic markers, such as mitochondrial DNA and Y chromosome sequences. This low genetic diversity of the wild Tsushima leopard cat may be derived from the founding population. Furthermore, our results suggest that the captive populations held in Japanese zoos may show extremely low genetic diversity, leading to difficulties in genetic management of the Tsushima leopard cat. Moreover, the two regional populations were clearly separated using these marker sets. In the present study, we demonstrated that the genetic diversity of the Tsushima leopard cat is extremely low compared with that of the continental regional population. Importantly, the Japanese captive population for ex situ conservation was derived from a founding population with extremely low genetic diversity; hence, we assume that both the captive and wild populations showed extremely low genetic diversities. Our findings emphasize the need to develop carefully considered management strategies for genetic conservation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Tsushima leopard cat exhibits extremely low genetic diversity compared with the Korean Amur leopard cat: Implications for conservation

Itô

Inoue‐Murayama

2019

PeerJ

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Patterns and drivers of genetic diversity among Felidae species

Azizan

Paradis

2021

Biodivers Conserv

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Microsatellite DNA analysis reveals lower than expected genetic diversity in the threatened leopard cat (Prionailurus bengalensis) in South Korea

Seomun

et al. 2018

Genes Genom

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To optimize conservation efforts, it is necessary to determine the risk of extinction by collecting reliable population information for a given species. We developed eight novel, polymorphic microsatellite markers and used these markers in conjunction with twelve existing markers to measure genetic diversity of South Korean populations of leopard cat (Prionailurus bengalensis), a species for which population size and habitat area data are unknown in the country, to assess its conservation status. The average number of alleles and the observed heterozygosity of the species were 3.8 and 0.41, respectively, and microsatellite diversity was lower than the average genetic diversity of 57 populations of 12 other felid species, and lower than that of other mammal populations occurring in South Korea, including the raccoon dog (Nyctereutes procyonoides), water deer (Hydropotes inermis), and endangered long-tailed goral (Naemorhedus caudatus). Furthermore, analysis of genetic structure in the national leopard cat population showed no clear genetic differentiation, suggesting that it is not necessary to divide the South Korean leopard cat population into multiple management units for the purposes of conservation. These results indicate that the genetic diversity of the leopard cat in South Korea is unexpectedly low, and that the risk of local extinction is, as a result, substantial. Thus, it is necessary to begin appropriate conservation efforts at a national level to conserve the leopard cat population in South Korea.

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A set of microsatellite markers for population genetics of leopard cat (Prionailurus bengalensis) and cross-species amplification in other felids

Cited by 9 publications

References 17 publications

The Tsushima leopard cat exhibits extremely low genetic diversity compared with the Korean Amur leopard cat: Implications for conservation

The Tsushima leopard cat exhibits extremely low genetic diversity compared with the Korean Amur leopard cat: Implications for conservation

Patterns and drivers of genetic diversity among Felidae species

Microsatellite DNA analysis reveals lower than expected genetic diversity in the threatened leopard cat (Prionailurus bengalensis) in South Korea

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