A set of PCRs for rapid identification and characterization of<i>Pseudomonas syringae</i>phylogroups

Borschinger, Benoit; Bartoli, Claudia; Chandeysson, Charlotte; Guilbaud, Caroline; Parisi, Luciana; Bourgeay, Jean-François; Buisson, Élise; Morris, Cindy E.

doi:10.1111/jam.13017

Cited by 25 publications

(7 citation statements)

References 42 publications

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“…Recently other workers have examined Psa isolates from kiwifruit and compared these with environmental pseudomonads and pseudomonads from woody hosts including Prunus domestica (plum) and P. armenica (apricot) using MLSA (Bartoli et al 2015). This work led to the identification of gene regions that were used to design polymerase chain reaction (PCR) primers that could distinguish between phylogroups of Pseudomonas syringae (Borschinger et al 2016). Furthermore, this analysis showed that strains isolated from the environment could be pathogenic on economically important hosts (Bartoli et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Using multilocus sequence analysis to distinguish pathogenic from saprotrophic strains of Pseudomonas from stone fruit and kiwifruit

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Using multilocus sequence analysis to distinguish pathogenic from saprotrophic strains of Pseudomonas from stone fruit and kiwifruit

et al. 2019

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“…oryzae 1_6). These strains represent much of the known phylogenetic breadth of plant pathogenic strains commonly included within the species P. syringae , although some have been taxonomically reclassified but are still considered P. syringae sensu lato ( Sarkar and Guttman, 2004 ; Bull et al, 2011 ; Borschinger et al, 2016 ). Additionally, two strains thought to be non-pathogenic on plants (Cit7 and TLP2) are included.…”

Section: Methodsmentioning

confidence: 99%

Entomopathogenicity to Two Hemipteran Insects Is Common but Variable across Epiphytic Pseudomonas syringae Strains

2017

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Strains of the well-studied plant pathogen Pseudomonas syringae show large differences in their ability to colonize plants epiphytically and to inflict damage to hosts. Additionally, P. syringae can infect some sap-sucking insects and at least one P. syringae strain is highly virulent to insects, causing death to most individuals within as few as 4 days and growing to high population densities within insect hosts. The likelihood of agricultural pest insects coming into contact with transient populations of P. syringae while feeding on plants is high, yet the ecological implications of these interactions are currently not well understood as virulence has not been tested across a wide range of strains. To investigate virulence differences across strains we exposed the sweet potato whitefly, Bemisia tabaci, and the pea aphid, Acyrthosiphon pisum, both of which are cosmopolitan agricultural pests, to 12 P. syringae strains. We used oral inoculations with bacteria suspended in artificial diet in order to assay virulence while controlling for other variables such as differences in epiphytic growth ability. Generally, patterns of pathogenicity remain consistent across the two species of hemipteran insects, with bacterial strains from phylogroup II, or genomospecies 1, causing the highest rate of mortality with up to 86% of individuals dead after 72 h post infection. The rate of mortality is highly variable across strains, some significantly different from negative control treatments and others showing no discernable difference. Interestingly, one of the most pathogenic strains to both aphids and whiteflies (Cit7) is thought to be non-pathogenic on plants. We also found Cit7 to establish the highest epiphytic population after 48 h on fava beans. Between the nine P. syringae strains tested for epiphytic ability there is also much variation, but epiphytic ability was positively correlated with pathogenicity to insects, suggesting that the two traits may be linked and that strains likely to be found on plants may often be entomopathogenic. Our study highlights that there may be a use for epiphytic bacteria in the biological control of insect crop pests. It also suggests that interactions with epiphytic bacteria could be evolutionary and ecological drivers for hemipteran insects.

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“…The internal tissues of 30 buds from each scion variety were analysed using culture-dependent methods. Based on the colony morphology, Pseudomonas-like colonies from each sample were cultured on King's B medium and evaluated in a hypersensitive reaction test on tobacco and for ice nucleation activity and determined by the FAME method [1][2][3]. A subset of strains determined by FAME as belonging to the genus Pseudomonas with a similarity index of SimIndex≥0.5 and/or strains with positive HR and INA within (0 °C; -6 °C) were attributed to the phylogroups of Pseudomonas syringae (Ps) complex and their pathogenicity on detached apricot twigs was evaluated [4,5].…”

Section: Methodsmentioning

confidence: 99%

The Close Relationship between the Careless Production of New Apricot Trees and the Spread of a Causal Agent of Bacterial Canker in Apricot Orchards

Pánková¹

2021

MCDA

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A set of PCRs for rapid identification and characterization ofPseudomonas syringaephylogroups

Cited by 25 publications

References 42 publications

Using multilocus sequence analysis to distinguish pathogenic from saprotrophic strains of Pseudomonas from stone fruit and kiwifruit

Using multilocus sequence analysis to distinguish pathogenic from saprotrophic strains of Pseudomonas from stone fruit and kiwifruit

Entomopathogenicity to Two Hemipteran Insects Is Common but Variable across Epiphytic Pseudomonas syringae Strains

The Close Relationship between the Careless Production of New Apricot Trees and the Spread of a Causal Agent of Bacterial Canker in Apricot Orchards

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