A set of recombinant antigens from <i>Echinococcus granulosus</i> with potential for use in the immunodiagnosis of human cystic hydatid disease

Virginio, Veridiana Gomes; Hernández, Ana; Rott, Marilise Brittes; Monteiro, Karina Mariante; Zandonai, Aline Fraga; Nieto, Alberto; Zaha, Arnaldo; Ferreira, Henrique Bunselmeyer

doi:10.1046/j.1365-2249.2003.02123.x

Cited by 101 publications

(110 citation statements)

References 46 publications

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“…The major drawback of these tests is the lack of species specificity, as cross-reactions between AE and CE and many other helminthic infections are commonly observed (cestodes, 89%; nematodes, 39%; trematodes; 30%) [5,20,[37][38][39][40]. Assays based on a recombinant subunit of AgB, denoted rAgB8/2, showed sensitivities of 45-93% and specificities of 86-99% with the same cross-reactions as mentioned above [20,41,42].…”

Section: Discussionmentioning

confidence: 99%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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PURPOSE: Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. METHODS: The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. RESULTS: According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and >99.9% for the diagnosis of CE and AE, respectively. Antigens derived from E. multilocularis metacestodes had sensitivities ranging from 84 to 91% and >99.9% for the diagnosis of CE and AE, respectively. Specificities ranged from 92 to >99.9%. Post-test probabilities for the differential diagnosis of AE from liver neoplasias, CE from cystic liver lesions, and screening for AE in Switzerland were around 95, 86 and 2.2%, respectively. Cross-reactions with antibodies in sera of patients with other parasitic affections (fasciolosis, schistosomosis, amebosis, cysticercosis, and filarioses) did occur at variable frequencies, but could be eliminated through the use of confirmatory testing. CONCLUSIONS: Different metacestode-derived antigens of E. granulosus and E. multilocularis are valuable, widely accessible, and cost-efficient tools for the serological diagnosis of echinococcosis. However, confirmatory testing is necessary, due to the lack of species specificity and the occurrence of cross-reactions to other helminthic diseases. Abstract Purpose Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. Methods The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. Results According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and [99.9% for the diagnosis of CE and AE, respectively. Antigens derived from...

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Section: Discussionmentioning

confidence: 99%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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“…Several recombinant antigens and synthetic peptides have been used for CE serodiagnosis in humans. [9][10][11][12][15][16][17][18] A highly antigenic protein, named EPC1, was characterized and isolated from a protoscolex (larval) stage and is encoded by the EPC1 gene. 13,17 Our previous study was aimed to assess the sequence of EPC1 isolated from different intermediate hosts of E. granulosus, including sheep (G1 strain) and camel (G6 strain).…”

Section: Discussionmentioning

confidence: 99%

“…Because of the association-specific response to antigens of E. granulosus, it can reduce cross-reactivity, whereas IgG4 recognizes antigen B or other immunomodulatory molecules; therefore, it would be a choice for serodiagnostic tests of complementary value associated with a total IgG assay. 12,[20][21][22][23][24] rEPC1 has a relatively promising performance in total IgG ELISA for the detection of antibodies in sera from patients with CE.…”

Section: Discussionmentioning

confidence: 99%

“…Several recombinant antigens and synthetic peptides (combining several defined antigens) have shown potential for CE serodiagnosis in humans. [9][10][11][12] On the other hand, the identification of infection in dogs is also important. A recombinant antigen, EPC1, an 8.5-kDa antigen from E. granulosus has been shown to be efficient in diagnosis of human hydatidosis.…”

Section: Introductionmentioning

confidence: 99%

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A Study of Cross-Reactivity Between Recombinant EPC1 Antigen of Echinococcus granulosus in Serum from Patients with Confirmed Cystic Echinococcosis Infection and Other Parasitic Infections

Fathi¹,

Jalousian²,

Hosseini³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. A standardized test for the serodiagnosis of cystic echinococcosis (CE) remains an important challenge because of the problems in specificity and sensitivity of the available commercial kits and lack of proper evaluation of antigen. Using appropriate sources of antigenic material is crucial in improvement of the serological methods such as enzyme-linked immunosorbent assay (ELISA). This study was conducted to evaluate the performance of protein named Echinococcus protoscolex calcium binding protein EPC1 for the detection of antibodies in sera from patients with CE. Expressed and purified recombinant protein EPC1 (rEPC1) was used as antigen in ELISA method. Characterization of the rEPC1 antigen was evaluated using the serum of 25 patients with both surgical and imaging confirmed CE and 25 healthy donors as negative controls. Also, a panel of sera including chronic toxoplasmosis (IgG positive), strongyloidosis, fascioliasis, toxocariasis, and kala azar were used and patients with related parasites were confirmed by medical laboratories or clinically by research centers using microscopy or specific ELISA. rEPC1 showed relatively promising performance in total IgG ELISA for the detection of antibodies in sera from the negative controls, and the cut off value 0.4 units of optical density at 490 nm was calculated for ELISA. In this study, sensitivity of 100%, specificity of 93.7, positive predictive value of 92.6%, and negative predictive value of 100% were calculated for rEPC1. On the other hand, commercial ELISA kit based on the native antigen B of Echinococcus granulosus had sensitivity of 96.2% and specificity of 96.8%. No significant difference was found for sensitivity or specificity between the rEPC1 and commercial kit. However, rEPC1 may be a valuable antigen for diagnosis of human CE.

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“…Accordingly, AgB has received much attention due to its high immunogenicity and specificity [8].Antigen B is encoded by a multigene family-EgAgB8/1, EgAgB8/2, EgAgB8/3, and EgAgB8/4, each of which codes for one subunit of 8 kDa [9,10]. E.granulosus enhances both humoral and cellular responses in its intermediate host.…”

Section: Introductionmentioning

confidence: 99%

Efficiency Evaluation of Immunization Protocol with Echinococcus Granulosus Antigen B (Agb)

Mustafa¹,

Shani²

2017

Cihan Univ.-Erbil Sci. J.

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Antigen B (AgB), the major antigens of Echinococcus granulosus, is a polymeric thermostable lipoprotein that constitutes as much as 10% of hydatid fluid.Antibody and cytokine response were both studied in rabbits immunized with Echinococcus granulosus antigen B (AgB). This study aimed to assess the effect of antigen B (AgB) on immune response for this purpose hydatid cysts were collected from liver of infected sheep from slaughter house in Basra . AgB was purified from hydatid fluid by anion exchange chromatography.For evaluation of AgB efficiency in immunization , 15 rabbits were enrolled in the study ,10 of them were immunized subcutaneously with AgB using freund's complete adjuvant after two weeks three booster injections were repeated intramuscularly according to immunization schedule using freund's incomplete adjuvant and 5 rabbits were treated as a control group. After six weeks , blood were collected from rabbits and the levels of IgG , IFN-γ and IL-10 were measured by ELISA test.Means of IgG of immunized rabbits were significantly (P < 0.05) elevated (45.9) ng/ml in comparison with control rabbits (16.6) ng/ml. Cytokines levels of Th1 (IFN-γ) and Th2 (IL-10) were recorded a significant differences (P < 0.05) between immunized and control groups , the mean concentrations of IFN-γ in immunized rabbits (28.6 pg/ml) was lower than those of control group (83.6 pg/ml) while IL-10 mean concentrations in immunized rabbits (60.1) pg/ml was higher than those of control group (18.8) pg/ml .The results indicated that AgB has the ability to modulate immune response toward Th2 bias in immunized rabbits as there was significant elevation in total IgG, IL-10 and significant decreasing IFN-γ concentration.

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A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease

Cited by 101 publications

References 46 publications

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

A Study of Cross-Reactivity Between Recombinant EPC1 Antigen of Echinococcus granulosus in Serum from Patients with Confirmed Cystic Echinococcosis Infection and Other Parasitic Infections

Efficiency Evaluation of Immunization Protocol with Echinococcus Granulosus Antigen B (Agb)

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