Abstract-Self-heating in a 0.25 μm BiCMOS technology with different isolation structures, including shallow and deep trenches on bulk and silicon-on-insulator (SOI) substrates is characterized experimentally. Thermal resistance values for single-and multi-finger emitter devices are extracted and compared to results obtained from 2-D fully-coupled electrothermal simulations. The difference in thermal resistance between the investigated isolation structures becomes more important for transistors with a small aspect ratio, i.e., short emitter length. The influence of thermal boundary conditions, including the substrate thermal resistance, the thermal resistance of the first metallization/via layer, and the simulation structure width is investigated. In the device with full dielectric isolation -deep polysiliconfilled trenches on an SOI substrate -accurate modelling of the heat flow in the metallization is found to be crucial. Furthermore, the simulated structure must be made wide enough to account for the large heat flow in the lateral direction.