A Short History and Perspectives on Plant Genetic Transformation

Ramkumar, Thakku R.; Lenka, Sangram K.; Arya, Sagar S.; Bansal, K. C.

doi:10.1007/978-1-0716-0356-7_3

Cited by 37 publications

(17 citation statements)

References 252 publications

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“…The Vn‐ and Vc‐fused BiFC vector modules enable the N‐ and C‐terminal fusion of two genes of interest to each tag, which could be used for (i) PPI analysis of two proteins of interest, (ii) colocalization analysis of two interacting proteins with organelle markers or (iii) colocalization analysis of two interacting proteins with another protein. The most commonly used approaches for introducing these plasmids into plant cells are PEG/Ca‐mediated transient transformation of protoplasts, biolistic‐mediated transient/stable transformation and Agrobacterium ‐mediated transient/stable transformation (Chen et al ., 2006 ; Jeong et al ., 2021 ; Ramkumar et al ., 2020 ). Of these techniques, protoplast transformation is a rapid and efficient method that has become a widely used tool for plant biology studies (He et al ., 2016 ; Yoo et al ., 2007 ).…”

Section: Discussionmentioning

confidence: 99%

All‐in‐one: a robust fluorescent fusion protein vector toolbox for protein localization and BiFC analyses in plants

Han

et al. 2022

Plant Biotechnology Journal

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Fluorescent tagging protein localization (FTPL) and bimolecular fluorescence complementation (BiFC) are popular tools for in vivo analyses of the subcellular localizations of proteins and protein-protein interactions in plant cells. The efficiency of fluorescent fusion protein (FFP) expression analyses is typically impaired when the FFP genes are co-transformed on separate plasmids compared to when all are cloned and transformed in a single vector. Functional genomics applications using FFPs such as a gene family studies also often require the generation of multiple plasmids. Here, to address these needs, we developed an efficient, modular all-in-one (Aio) FFP (AioFFP) vector toolbox, including a set of fluorescently labelled organelle markers, FTPL and BiFC plasmids and associated binary vectors. This toolbox uses Gibson assembly (GA) and incorporates multiple unique nucleotide sequences (UNSs) to facilitate efficient gene cloning. In brief, this system enables convenient cloning of a target gene into various FFP vectors or the insertion of two or more target genes into the same FFP vector in a single-tube GA reaction. This system also enables integration of organelle marker genes or fluorescently fused target gene expression units into a single transient expression plasmid or binary vector. We validated the AioFFP system by testing genes encoding proteins known to be functional in FTPL and BiFC assays. In addition, we performed a high-throughput assessment of the accurate subcellular localizations of an uncharacterized rice CBSX protein subfamily. This modular UNS-guided GAmediated AioFFP vector toolkit is cost-effective, easy to use and will promote functional genomics research in plants.

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Section: Discussionmentioning

confidence: 99%

All‐in‐one: a robust fluorescent fusion protein vector toolbox for protein localization and BiFC analyses in plants

Han

et al. 2022

Plant Biotechnology Journal

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“…Unlike any other model or major crop species from 1983 (Ramkumar et al 2020 ), a complete sugarcane genetic transformation and plant regeneration system was established a decade later (Bower and Birch 1992 ), even though the transformation of protoplast and cell suspension culture were reported earlier. This delay is a reflection of the lack of a plant regeneration system, which was established later.…”

Section: Genetic Transformations and Crop Resiliencementioning

confidence: 99%

A short review on sugarcane: its domestication, molecular manipulations and future perspectives

Babu

Janakiraman

Palaniswamy

et al. 2022

Genet Resour Crop Evol

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Sugarcane ( Saccharum spp.) is a special crop plant that underwent anthropogenic evolution from a wild grass species to an important food, fodder, and energy crop. Unlike any other grass species which were selected for their kernels, sugarcane was selected for its high stem sucrose accumulation. Flowering in sugarcane is not favored since flowering diverts the stored sugar resources for the reproductive and developmental energy needs. Cultivars are vegetatively propagated and sugarcane breeding is still essentially focused on conventional methods, since the knowledge of sugarcane genetics has lagged that of other major crops. Cultivar improvement has been extremely challenging due to its polyploidy and aneuploidy nature derived from a few interspecific hybridizations between Saccharum officinarum and Saccharum spontaneum, revealing the coexistence of two distinct genome organization modes in the modern variety. Alongside implementation of modern agricultural techniques, generation of hybrid clones, transgenics and genome edited events will help to meet the ever-growing bioenergy needs. Additionally, there are two common biotechnological approaches to improve plant stress tolerance, which includes marker-assisted selection (MAS) and genetic transformation. During the past two decades, the use of molecular approaches has contributed greatly to a better understanding of the genetic and biochemical basis of plant stress-tolerance and in some cases, it led to the development of plants with enhanced tolerance to abiotic stress. Hence, this review mainly intends on the events that shaped the sugarcane as what it is now and what challenges ahead and measures taken to further improve its yield, production and maximize utilization to beat the growing demands.

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“…Plant genetic transformation refers to the generation of transgenic plants (transgenics), which harbor extra, stably integrated, and expressed foreign gene(s) generally from trans species ( Figure 2 e) [ 135 , 136 ]. These modified plants are generally named genetically modified organisms (GMOs).…”

Section: Molecular Toolsmentioning

confidence: 99%

Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops

Villalobos-López

Arroyo-Becerra

Quintero-Jiménez

et al. 2022

IJMS

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The major challenges that agriculture is facing in the twenty-first century are increasing droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate change. However, most crops are not tolerant to extreme climatic environments. The aim in the near future, in a world with hunger and an increasing population, is to breed and/or engineer crops to tolerate abiotic stress with a higher yield. Some crop varieties display a certain degree of tolerance, which has been exploited by plant breeders to develop varieties that thrive under stress conditions. Moreover, a long list of genes involved in abiotic stress tolerance have been identified and characterized by molecular techniques and overexpressed individually in plant transformation experiments. Nevertheless, stress tolerance phenotypes are polygenetic traits, which current genomic tools are dissecting to exploit their use by accelerating genetic introgression using molecular markers or site-directed mutagenesis such as CRISPR-Cas9. In this review, we describe plant mechanisms to sense and tolerate adverse climate conditions and examine and discuss classic and new molecular tools to select and improve abiotic stress tolerance in major crops.

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A Short History and Perspectives on Plant Genetic Transformation

Cited by 37 publications

References 252 publications

All‐in‐one: a robust fluorescent fusion protein vector toolbox for protein localization and BiFC analyses in plants

All‐in‐one: a robust fluorescent fusion protein vector toolbox for protein localization and BiFC analyses in plants

A short review on sugarcane: its domestication, molecular manipulations and future perspectives

Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops

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