This study investigated eco-friendly antibacterial medical protective clothing via the nonwoven process and characteristic evaluations. Firstly, Tencel® fibers and low melting point polyester (LMPET) fibers (re-sliced and granulated from recycled PET bottles) were mixed at different ratios and then needle punched at diverse needle rolling depths. The influences of manufacturing parameters on the Tencel®/LMPET nonwoven fabrics were examined in terms of mechanical properties, water vapor transmission rate, and stiffness. Next, Tencel®/LMPET nonwoven fabrics were combined with thermoplastic polyurethane (TPU)/Triclosan antibacterial membranes that contained different contents of triclosan using melt processing technology. The resulting Tencel®/LMPET/TPU/Triclosan composites were characterized via different measurements; an optimal bursting strength of 86.86 N, an optimal horizontal tensile strength of 41.90 N, and an optimal stiffness along the MD and CD of 8.60 cm were recorded. Furthermore, the Tencel®/LMPET/TPU/Triclosan composites exhibited a distinct inhibition zone in the antibacterial measurement, and the hydrostatic pressure met the requirements of the EN 14126:2003 and GB 19082-200 disposable medical protective gear test standards.