A short treatment of cells with the lanthanide ions La3+, Ce3+, Pr3+ or Nd3+ changes the cellular chemistry into a state in which RNA replication of flaviviruses is specifically blocked without interference with host-cell multiplication

Abstract

Alpha- and flaviviruses contain class II fusion proteins, which form ion-permeable pores in the target membrane during virus entry. The pores generated during entry of the alphavirus Semliki Forest virus have been shown previously to be blocked by lanthanide ions. Here, analyses of the influence of rare earth ions on the entry of the flaviviruses West Nile virus and Uganda S virus revealed an unexpected effect of lanthanide ions. The results showed that a 30 s treatment of cells with an appropriate lanthanide ion changed the cellular chemistry into a state in which the cells no longer supported the multiplication of flaviviruses. This change occurred in cells treated before, during or after infection, did not inhibit multiplication of Semliki Forest virus and did not interfere with host-cell multiplication. The change was generated in vertebrate and insect cells, and was elicited in the presence of actinomycin D. In vertebrate cells, the change was elicited specifically by La3+, Ce3+, Pr3+ and Nd3+. In insect cells, additional lanthanide ions had this activity. Further analyses showed that lanthanide ion treatment blocked the ability of the host cell to support the replication of flavivirus RNA. These results open two areas of research: the study of molecular alterations induced by lanthanide ion treatment in uninfected cells and the analysis of the resulting modifications of the flavivirus RNA replicase complex. The findings possibly open the way for the development of a general chemotherapy against flavivirus diseases such as Dengue fever, Japanese encephalitis, West Nile fever and yellow fever.

Effects of cerium and tungsten substitution on antiviral and antibacterial properties of lanthanum molybdate

“…Wengler et al also demonstrated that lanthanide ion (such as La(III) and Ce(III)) treatment blocks the ability of the host cell to support the replication of flavivirus (envelope type virus) RNA. A similar effect of La(III) is expected to contribute to the inactivation of Qβ [ 45 ]. The slight antiviral activity shown by CeO 2 might be attributable to the major Ce ion valence (not III but IV).…”

Development of Functional Bionanocomposites Using Cyanobacterial Polysaccharides

“…This photo‐shrinkage of the sacran gels may be attributed to the hydrophobization of uronic acid based on photo‐decarboxylation. These phenomena could be seen in other trivalent ions whose controlled release might be effective in cancer targeting (if radioactive ions were used) and virus infection control . Furthermore, any polysaccharides containing hexose can be materialized using the present photoreaction‐mediated phenomenon because uronic acid can be easily converted from hexose by oxidation.…”

The cyanobacterial polysaccharide sacran: characteristics, structures, and preparation of LC gels

“…This photoshrinking is expected to occur in a mixture with other trivalent ions. It is expected that, by using the composites, controlled release will be useful in targeted cancer therapies [47] and virus infection controls [48].…”