A shortened resynchronization treatment for dairy cows after a nonpregnancy diagnosis

Sauls-Hiesterman, J.A.; Voelz, B.E.; Stevenson, Jeffrey S.

doi:10.1016/j.theriogenology.2019.09.013

Cited by 14 publications

(9 citation statements)

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“…Initiating the resynchronization protocol at NPD diagnosis either at d32 or d39 after a previous AI did not differ in terms of P/AI [40,110]. However, presynchronization with a GnRH treatment 7 days before the onset of the protocol improved fertility [40,110], and P4 supplementation increased P/AI, especially in cows without CL or with P4 < 1.0 ng/mL at the beginning of the resynchronization protocol [109,110].…”

Section: Reinsemination Strategies: Reducing the Interval Between Ai And Optimizing Fertilitymentioning

confidence: 80%

“…It is common to initiate the resynchronization protocol at the time of the NPD. However, there are strategies that initiate the TAI protocol before NPD, which include presynchronization protocols or use P4 supplementation [40,41,[102][103][104][105][106][107][108][109].…”

Section: Reinsemination Strategies: Reducing the Interval Between Ai And Optimizing Fertilitymentioning

confidence: 99%

“…A more recent study [109] compared Resynch-32 (with two PGF treatments) to a management strategy in which nonpregnant cows with CL at NPD received two PGF 24 h apart, and GnRH 16 before TAI (on Day 35), while cows without CL at NPD were enrolled in a Ovsynch + P4 protocol, with two PGF at the end, and were reinseminated 42 days after a previous AI. The authors reported that the management strategy that evaluated ovarian structures to enroll cows in the selected Resynch strategies was a viable alternative to reduce inter-insemination intervals [109].…”

Section: Reinsemination Strategies: Reducing the Interval Between Ai And Optimizing Fertilitymentioning

confidence: 99%

See 2 more Smart Citations

Factors That Optimize Reproductive Efficiency in Dairy Herds with an Emphasis on Timed Artificial Insemination Programs

Consentini

Wiltbank

Sartori

2021

Animals

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Reproductive efficiency is closely tied to the profitability of dairy herds, and therefore successful dairy operations seek to achieve high 21-day pregnancy rates in order to reduce the calving interval and days in milk of the herd. There are various factors that impact reproductive performance, including the specific reproductive management program, body condition score loss and nutritional management, genetics of the cows, and the cow comfort provided by the facilities and management programs. To achieve high 21-day pregnancy rates, the service rate and pregnancy per artificial insemination (P/AI) should be increased. Currently, there are adjustments in timed artificial insemination (TAI) protocols and use of presynchronization programs that can increase P/AI, even to the point that fertility is higher with some TAI programs as compared with AI after standing estrus. Implementation of a systematic reproductive management program that utilizes efficient TAI programs with optimized management strategies can produce high reproductive indexes combined with healthy cows having high milk production termed “the high fertility cycle”. The scientific results that underlie these concepts are presented in this manuscript along with how these ideas can be practically implemented to improve reproductive efficiency on commercial dairy operations.

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Section: Reinsemination Strategies: Reducing the Interval Between Ai And Optimizing Fertilitymentioning

confidence: 80%

Section: Reinsemination Strategies: Reducing the Interval Between Ai And Optimizing Fertilitymentioning

confidence: 99%

Section: Reinsemination Strategies: Reducing the Interval Between Ai And Optimizing Fertilitymentioning

confidence: 99%

See 1 more Smart Citation

Factors That Optimize Reproductive Efficiency in Dairy Herds with an Emphasis on Timed Artificial Insemination Programs

Consentini

Wiltbank

Sartori

2021

Animals

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“…For instance, Kelton et al (1991) reported that the sensitivity and specificity of transrectal palpation for diagnosing a functional CL were 82.6 and 52.6%, respectively. Similarly, Bicalho et al (2008) and Sauls-Hiesterman et al (2020) reported that the sensitivity and specificity for transrectal ultrasonography were approximately 90 and 40%, respectively. Because our LFIA system predicts the presence of a functional CL based on P4 levels rather than rectal palpation of the ovaries or visual observation of luteal tissue by ultrasonography, the number of false positives may be reduced, which may explain the substantially greater specificity compared with reported values for some of the traditional methods to detect the presence of a functional CL.…”

Section: Resultsmentioning

confidence: 88%

A lateral flow-based portable platform for determination of reproductive status of cattle

Masello

Erickson

et al. 2020

Journal of Dairy Science

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Our objective was to develop and validate a tool integrating a disposable fluorescence-based lateral flow immunoassay (LFIA) coupled with a portable imaging device for estimating circulating plasma concentrations of progesterone (P4). First, we developed and optimized a competitive LFIA test strip to measure P4 in bovine plasma. The LFIA design included a sample pad, a conjugate pad that stores R-phycoerythrin-anti-P4 conjugates, a glass-fiber spacer pad, a nitrocellulose membrane with printed test and control lines, and a cellulose-fiber absorbent pad. To perform a test, 20 µL of plasma and 50 µL of running buffer were added on the sample pad. After 3 min, 45 µL of running buffer was added to initiate sample flow. After allowing 15 min to stabilize the colorimetric signal, strips were introduced in an LFIA portable reader wirelessly linked to a laptop to determine P4 concentration based on test-to-controlline signal (T/C ratio). In a series of experiments (n = 6), the ability of the LFIA to differentiate plasma samples with ≥1 or <1 ng/mL of P4 was evaluated. For each experiment, a calibration curve was constructed using plasma with known concentrations of P4 (0.1 to 3.7 ng/mL; n = 5). The resulting linear equation was then used to determine a T/C ratio cutoff to differentiate samples with ≥1 or <1 ng/mL of P4. In addition, to evaluate the ability of the platform to assign samples to P4 concentration groups without a calibration curve for individual batches, we performed a receiver operating characteristic analysis to identify a single cutoff value for T/C ratio that could potentially be used for all batches. Overall, calibration curves showed a linear relationship between T/C ratio and P4 levels (mean coefficient of determination = 0.74; range 0.42 to 0.99). Next, plasma samples from lactating dairy cows (n = 58) were tested in triplicate to determine the ability of the LFIA system to differentiate plasma samples with ≥1 or <1 ng/mL of P4 using a RIA for P4 as reference test. Overall, the LFIA assay correctly classified 90% of the samples, with 97% sensitivity, 83% specificity, 85% positive predictive value, and 96% negative predictive value. Agreement between the tests was substantial (kappa = 0.79; 95% confidence interval 0.64 to 0.95). When using a single cutoff value for T/C ratio selected by receiver operating characteristic analysis, sensitivity and specificity to determine CL presence were 97 (95% confidence interval 82 to 99) and 79% (95% confidence interval 60 to 92), respectively. These data suggest that the developed portable LFIA system can accurately differentiate plasma samples with ≥1 or <1 ng/mL of P4.

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“…Esse aumento resulta em um grande ganho econômico para a fazenda. A PGF2α e seus análogos sintéticos são comumente utilizados em protocolos hormonais de bovinos, especialmente quando se utiliza programas de transferência de embriões em tempo fixo (Sauls-Hiesterman, Voelz, & Stevenson, 2020). A utilização desse hormônio tem como um dos principais efeitos a indução da luteólise, ou seja, destruição do corpo lúteo (Gaebler et al, 2015;Barreiros et al, 2006).…”

Section: Resultsunclassified

Estratégia de protocolo hormonal baseado na antecipação de PGF2α para melhorar a eficiência reprodutiva em vacas leiteiras submetidas a transferência de embriões em tempo fixo (TETF)

Medeiros

Câmara

Souza

et al. 2021

RSD

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Esse estudo objetivou comparar dois protocolos hormonais para transferência de embriões em tempo fixo (TETF) em vacas de leite baseados no momento da aplicação de PGF2α. Um total de 2.172 vacas mestiças Bos indicus foram utilizadas. De acordo com o protocolo de sincronização das receptoras de embriões, as vacas foram divididas em dois tratamentos: o Convencional e o MMSINCRO (tratamento teste). O protocolo Convencional consistiu na inserção de um dispositivo contendo progesterona e administração de benzoato de estradiol no Dia 0. No Dia 8, a progesterona foi removida e aplicado PGF2α, eCG e cipionato de estradiol. No Dia 10, os animais receberam GnRH. A TETF foi realizada no Dia 17. O MMSINCRO consistiu na inserção da progesterona e administração de benzoato de estradiol no Dia 0. No Dia 7, animais receberam PGF2α. No Dia 9, a progesterona foi removida e os animais receberam eCG e cipionato de estradiol. No dia 11, foi aplicado GnRH. A TETF foi realizada no Dia 18. A taxa de concepção das receptoras do tratamento MMSINCRO (52%) foi significativamente superior a do protocolo Convencional (33 %). A avaliação qualitativa do corpo lúteo mostrou uma melhor qualidade no tratamento MMSINCRO. Dessa forma, foi possível concluir que para TETF em vacas leiteira, aconselha-se a utilização do protocolo MMSINCRO nas receptoras, o qual foi capaz de aumentar a taxa de concepção. Esse achado proporciona uma melhora na eficiência reprodutiva de bovinos leiteiros, e consequentemente, em uma maior produção de leite e derivados nas fazendas de vacas leiteiras.

show abstract

A shortened resynchronization treatment for dairy cows after a nonpregnancy diagnosis

Cited by 14 publications

References 50 publications

Factors That Optimize Reproductive Efficiency in Dairy Herds with an Emphasis on Timed Artificial Insemination Programs

Factors That Optimize Reproductive Efficiency in Dairy Herds with an Emphasis on Timed Artificial Insemination Programs

A lateral flow-based portable platform for determination of reproductive status of cattle

Estratégia de protocolo hormonal baseado na antecipação de PGF2α para melhorar a eficiência reprodutiva em vacas leiteiras submetidas a transferência de embriões em tempo fixo (TETF)

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