A Simple 96-Well Microplate Method for Estimation of Total Polyphenol Content in Seaweeds

Zhang, Qing; Zhang, Junzeng; Shen, Jingkai; Silva, Angelica; Dennis, Dorothy; Barrow, Colin J.

doi:10.1007/s10811-006-9048-4

Cited by 386 publications

(142 citation statements)

References 22 publications

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“…It is commercially valuable as a feed supplement for agricultural animals and as a fertilizer ingredient for soil amendment, as well as having a history of use as a human foodstuff. Previously, we reported that A. nodosum is a rich source of polyphenols and has significant antioxidant scavenging activity toward the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical (Zhang et al 2006). A. nodosum and other brown seaweeds contain phlorotannins (compounds that have phloroglucinol as structural units and have a molecular size from tens of thousands to a million Da for polyphlorglucinols).…”

Section: Discussionmentioning

confidence: 99%

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Antidiabetic properties of polysaccharide- and polyphenolic-enriched fractions from the brown seaweedAscophyllum nodosumThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.

Zhang

Tiller

Shen

et al. 2007

Can. J. Physiol. Pharmacol.

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Abstract:We screened seaweed species from Atlantic Canada for antidiabetic activity by testing extracts for a-glucosidase inhibitory effect and glucose uptake stimulatory activity. An aqueous ethanolic extract of Ascophyllum nodosum was found to be active in both assays, inhibiting rat intestinal a-glucosidase (IC 50 =7 7mg/mL) and stimulating basal glucose uptake into 3T3-L1 adipocytes during a 20-minute incubation by about 3-fold (at 400 mg/mL extract). Bioassay-guided fractionation of the A. nodosum extract showed that a-glucosidase inhibition was associated with polyphenolic components in the extract. These polyphenolics, along with other constituents appeared to be responsible for the stimulatory activity on glucose uptake. However, attempts to further concentrate this activity through fractionation techniques were unsuccessful. A crude polyphenol extract (PPE), an enriched polyphenolic fraction (PPE-F1) and a polysaccharide extract (PSE) were prepared from commercial A. nodosum powder and administered to streptozotocin-diabetic mice for up to 4-weeks by daily gavage at 200 mg/kg body mass. PPE and PPE-F1 improved fasting serum glucose level in diabetic mice; however, the effect was only statistically significant at day 14. In addition, PPE-F1 was shown to blunt the rise in blood glucose after an oral sucrose tolerance test in diabetic mice. Mice treated with PPE and PPE-F1 had decreased blood total cholesterol and glycated serum protein levels compared with untreated diabetic mice, whereas PPE also normalized the reduction in liver glycogen level that occurred in diabetic animals. All 3 A. nodosum preparations improved blood antioxidant capacity.Key words: seaweed, Ascophyllum nodosum, a-glucosidase, diabetes.Résumé : On a établit une recherche d'un produit anti-diabétique, parmi les algues locales de la région Atlantique du Canada, en examinant la capacité d'un effet inhibiteur de l'enzyme a-glucosidase et une stimulation de l'incorporation cellulaire du glucose. Un extrait éthanol-aqueux de Ascophyllum nodosum nous a donné une activité positive chez les deux essais, une inhibition de l'a-glucosidase provenant de l'intestin du rat (IC50 =7 7mg/mL) et puis une stimulation triple, à une concentration de 400 mg/mL, de l'incorporation du glucose dans les adipocytes 3T3-L1 durant une période de 20 minutes. L'extrait de A. nodosum aété divisé, guidé par les résultats biologiques, et a ainsi démontré la présence d'éléments polyphénoliques associé à l'inhibition de l'a-glucosidase. Ces éléments polyphénoliques ainsi que d'autres semblent être responsables de l'incorporation stimulée du glucose. Il a été impossible de raffiner cette activité lors d'une division des composants. Un extrait brut polyphénolique (PPE), un extrait enrichi polyphénolique (PPE-F1) et puis un extrait polysaccharide (PSE) furent préparés d'une poudre commerciale de A. nodosum et utilisés dans une étude utilisant des souris, rendues diabétiques par injections de streptozotocin, traitées par un gavage journalier de l'extrait 200 mg/kg du poid...

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Section: Discussionmentioning

confidence: 99%

“…Total polyphenol content of samples was measured by the Folin-Ciocalteu method (Singleton et al 1999) modified for use with a 96-well microplate (Zhang et al 2006). Samples were run in duplicate.…”

Section: Polyphenol Contentmentioning

confidence: 99%

Antidiabetic properties of polysaccharide- and polyphenolic-enriched fractions from the brown seaweedAscophyllum nodosumThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.

Zhang

Tiller

Shen

et al. 2007

Can. J. Physiol. Pharmacol.

Self Cite

100

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“…Polyphenol content of dried fruit extracts was determined using the Folin-Ciocalteu method as described by Zhang et al (2006). The extracts (20 µl) and Folin-Ciocalteu reagents (100 µl) were mixed into 96-wellplate flat bottom and waited for 5 minutes before mixing it with 7.5% of sodium carbonate (80 µl).…”

Section: Determination Of Polyphenol Contentmentioning

confidence: 99%

Nutrient Compositions and Total Polyphenol Contents of Selected Dried Fruits Available in Selangor, Malaysia

Khairuddin¹,

Haron²,

Yahya³

et al. 2017

JAS

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Dried fruits contained higher amount of energy and nutrient density compared to the fresh ones. This study was conducted to determine and compare the content of nutrients and polyphenols in dried dates, raisin, apricot and fig. Dried fruit samples were bought from three different stores in Selangor and analyzed in triplicates. AOAC (1997) standard methods were used for proximate analyses. The total sugar and polyphenol contents were determined using Dubois and Folin-Ciocalteu methods, respectively. Highest moisture content was found in dried apricots (35.26%) while the lowest one was in Mariami dates (21

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“…Total Phenol content (TPC) of Curcuma longa rhizome ethanolic extract (CLRE) was determined by the Folin Denis calorimetric method using Folin-Ciocalteau reagent (Merck, Darmstadt, Germany) in Gallic acid equivalent (mg GAE/extract) ( Zhang et al, 2006). Curcuma longa rhizome ethanolic extract (1 mg) was first dissolved in 1 ml dimethyl sulfoxide (DMSO).…”

Section: Determination Of Total Phenolicsmentioning

confidence: 99%

Potential therapeutic effect of turmeric (Curcuma longa) against adverse effects of penconazole fungicide to white rats

Rasoul

Marei

2016

IJPT

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This study aimed to investigate the prophylactic effect of turmeric (Curcuma longa) Rhizome Ethanolic extract (CLRE) at 250 mg/kg as antioxidant effects against penconazole induced sub-acute toxicity. Hepatic, renal and testicular pathological changes caused by oxidative damage induced by penconazole in rats were biochemically and histologically evaluated. Male rats were treated with penconazole, via oral route, at doses of 0.5 mg/ kg body weight (b.w.; acute reference dose, ARfD), 25 mg/kg b.w. (no observed adverse effects level, NOAEL) and 100 mg/ kg b.w. (1/20 lethal dose [LD 50 ]) for 28 consecutive days. Penconazole treatments had significant (p < 0.05) and gradual reductions in body and relative testicular weight accompanied by significant elevation in the relative liver and kidney weights. Significant increase serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dhydrogenase(LDH), gamma-glutamyl transferase (GGT), creatinine (Cre), uric acid and blood glucose was observed due to penconazole treatments. However, total protein and testosterone hormone were significantly decreased. Exposure to penconazole caused increase in lipid peroxidation (LPO) and decreased of liver and kidney antioxidant enzymes activity as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). Histopathological studies confirmed the ameliorative beneficial effects of turmeric biochemical parameters. On the basis of this study, the use of tumeric rhizomes as a functional food or as a nutraceutical product could be a useful approach to protect individuals who are regularly exposed to penconazole.

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A Simple 96-Well Microplate Method for Estimation of Total Polyphenol Content in Seaweeds

Cited by 386 publications

References 22 publications

Antidiabetic properties of polysaccharide- and polyphenolic-enriched fractions from the brown seaweedAscophyllum nodosumThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.

Antidiabetic properties of polysaccharide- and polyphenolic-enriched fractions from the brown seaweedAscophyllum nodosumThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.

Nutrient Compositions and Total Polyphenol Contents of Selected Dried Fruits Available in Selangor, Malaysia

Potential therapeutic effect of turmeric (Curcuma longa) against adverse effects of penconazole fungicide to white rats

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