A simple and cost-effective method, as an appropriate alternative for visible spectrophotometry: development of a dopamine biosensor

Abstract

In this study, a new, simple, fast and inexpensive method as an alternative to visible spectrophotometry is developed. In this method the cells containing the sample solution were scanned with a scanner, then the color of each cell was analyzed with software written in visual basic (VB 6) media to red, green and blue values. The cells were built by creating holes in the Plexiglas sheet. The dimensions of identical cells were examined by Cr (III) solution with known concentrations. The validity of this new method was studied by determination of dopamine (DA) without using any other reagent. The parameters which affect the system were optimized. The comparison between the current and traditional UV-Vis spectrophotometry methods was studied and the results revealed similar trends in both methods. The developed method was successfully applied to the determination of dopamine in serum and urine without using any pretreatment. Finally comparing the results obtained in the developed method showed that microwave irradiation of the solution can decrease the experimental time, increase sensitivity and improve the limit of detection.

Application of graphene oxide/lanthanum-modified carbon paste electrode for the selective determination of dopamine

“…Abnormally high or low levels of DA can lead to a number of diseases, such as Alzheimer's disease, Parkinsonism, epilepsy, and HIV infection [3,4]. Conventional methods to detect DA include capillary electrophoresis [5], fluorometry [6,7], chromatography [8], spectrophotometry [9], and chemiluminescence [10]. Despite their many benefits, these methods generally demand extreme experimental conditions and complicated equipment.…”

Biomedical imaging and sensing using flatbed scanners

“…For example, they successfully quantified adsorbed elements and organic compounds on polyurethane foam with comparable results to traditional adsorption-spectrophotometric methods. 58–60 Also, flatbed scanners have been used in conjunction with colorimetric assays for pH monitoring 61,62 , and to develop a dopamine biosensor 63,64 . Another emerging application is scanner based digitization of paper based, inkjet printed, microfluidic devices to perform chemical sensing 65 and immunochemistry 66 .…”

Scanometry as microplate reader for high throughput method based on DPPH dry reagent for antioxidant assay

“…The scanometric technique which relies on either a light scattering instrument or flatbed scanner coupled with various probes or sensors can be used for the detection of bacteria, dopamine, magnesium ions, lead ions, thrombin, and mercury ions21, 22, 23, 24, 25, 26. Being subclass of colorimetry, scanometry uses a gray scale as opposed to the various color space.…”