A Simple and Cost-Efficient Platform for a Novel Porcine Circovirus Type 2d (PCV2d) Vaccine Manufacturing

Abstract

Porcine circovirus type 2d (PCV2d) is becoming the predominant PCV genotype and considerably affects the global pig industry. Nevertheless, currently, no commercial PCV2d vaccine is available. Preventing and controlling the disease caused by PCV2d is therefore based on other genotype-based vaccines. However, their production platforms are laborious, limited in expression level, and relatively expensive for veterinary applications. To address these challenges, we have developed a simple and cost-efficient platform for a novel PCV2d vaccine production process, using fed-batch E. coli fermentation followed by cell disruption and filtration, and a single purification step via cation exchange chromatography. The process was developed at bench scale and then pilot scale, where the PCV2d subunit protein yield was approximately 0.93 g/L fermentation volume in a short production time. Moreover, we have successfully implemented this production process at two different sites, in Southeast Asia and Europe. This demonstrates transferability and the high potential for successful industrial production.

Automated Shape and Process Parameter Optimization for Scaling Up Geometrically Non-Similar Bioreactors

“…As can be seen in Figure 3, the specific power inputs agree for the range from v tip = 2 m s −1 (N = 364 rpm and Re = 66,592) to v tip = 5 m s −1 (N = 909 rpm and Re = 166,481). As already mentioned, the 30 L D-DCU bioreactor is a bioreactor typically used for microorganisms [65,81,82]. Therefore Schirmer et al [65] only investigated the range from v tip = 2 m s −1 to 5 m s −1 , which corresponds to a specific power input over 1 kW m −3 .…”