A simple and effective method for making multipotent/multilineage scaffolds with hydrophilic nature without any postmodification/treatment

Vaikkath, Dhanesh; Anitha, R.; Sumathy, Babitha; Nair, Prabha D.

doi:10.1016/j.colsurfb.2015.12.041

Cited by 14 publications

(8 citation statements)

References 42 publications

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“…PCL/PCL‐PTHF‐PCL electrospun membranes have shown high hydrophilicity and support cell adhesion, proliferation and differentiation of mesenchymal stem cells as reported in our earlier studies (Vaikkath et al, ).…”

Section: Discussionsupporting

confidence: 72%

“…This high water absorption capability can be attributed to the hygroscopic nature of PCL-PTHF-PCL triblock copolymer and wicking action of more number of nanofibers in the lattice scaffold. This is highly advantageous for efficient nutrient and oxygen exchange for the cells cultured in these 3D scaffolds (Aloysious & Nair, 2014;Vaikkath et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…This is highly advantageous for efficient nutrient and oxygen exchange for the cells cultured in these 3D scaffolds (Aloysious & Nair, 2014;Vaikkath et al, 2016). This high water absorption capability can be attributed to the hygroscopic nature of PCL-PTHF-PCL triblock copolymer and wicking action of more number of nanofibers in the lattice scaffold.…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Rat adipose mesenchymal stem cells were isolated and cultured after obtaining consent from Institute Animal Ethics Committee and Institutional Committee for Stem cell research, according to our published protocol, in a humidified incubator at 37 C with 5% CO 2 (Vaikkath, Anitha, Sumathy, & Nair, 2016). The morphology and growth of the isolated cells were observed with a phase-contrast microscope (Olympus IX 71; Figure S5).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

“…In the current study, we chose a unique polymer blend that is hydrophilic and biocompatible (Vaikkath, Anitha, Sumathy, & Nair, 2016). This polymer blend was fabricated in the form of a 3D porous electrospun scaffold by a two-step method so as to overcome the limitation of cell infiltration, allow cell aggregation and formation of 3D clusters of islets after differentiation.…”

mentioning

confidence: 99%

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Tissue‐engineered islet‐like cell clusters generated from adipose tissue‐derived stem cells on three‐dimensional electrospun scaffolds can reverse diabetes in an experimental rat model and the role of porosity of scaffolds on cluster differentiation

Anitha

Vaikkath

Shenoy

et al. 2019

J Biomedical Materials Res

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In the current study, three-dimensional (3D) nanofibrous scaffolds with pore sizes in the range of 24-250 μm and 24-190 μm were fabricated via a two-step electrospinning method to overcome the limitation of obtaining three-dimensionality with large pore sizes for islet culture using conventional electrospinning. The scaffolds supported the growth and differentiation of adipose-derived mesenchymal stem cells to islet-like clusters (ILCs). The pore size of the scaffolds was found to influence the cluster size, viability and insulin release of the differentiated islets. Hence, islet clusters of the desired size could be developed for transplantation to overcome the loss of bigger islets due to hypoxia which adversely impacts the outcome of transplantation. The tissue-engineered constructs with ILC diameter of 50 μm reduced glycemic value within 3-4 weeks after implantation in the omental pouch of diabetic rats. Detection of insulin in the serum of implanted rats demonstrates that the tissue-engineered construct is efficient to control hyperglycemia. Our findings prove that the 3D architecture and pore size of scaffolds regulates the morphology and size of islets during differentiation which is critical in the survival and function of ILCs in vitro and in vivo. K E Y W O R D Sdiabetes, electrospun three-dimensional scaffolds, in vivo, islet tissue engineering, stem cells

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: In Vivo Studiesmentioning

confidence: 99%

Section: Cell Isolation and Culturementioning

confidence: 99%

mentioning

confidence: 99%

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Tissue‐engineered islet‐like cell clusters generated from adipose tissue‐derived stem cells on three‐dimensional electrospun scaffolds can reverse diabetes in an experimental rat model and the role of porosity of scaffolds on cluster differentiation

Anitha

Vaikkath

Shenoy

et al. 2019

J Biomedical Materials Res

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3D printing of poly(butylene adipate‐co‐terephthalate) (PBAT)/niobium containing bioactive glasses (BAGNb) scaffolds: Characterization of composites, in vitro bioactivity, and in vivo bone repair

Ulbrich

Balbinot

Brotto

et al. 2021

J Tissue Eng Regen Med

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This study aimed to produce poly(butylene adipate-co-terephthalate) (PBAT)/ niobium containing bioactive glasses (BAGNb) composites scaffolds produced by fused deposition modeling (FDM) printing and evaluate their physicochemical and biological properties in vitro and in vivo. The composite filaments were produced by melt-extrusion with the addition of 10 wt% of BAGNb (PBAT/BAGNb). Filaments without BAGNb were produced as the control group (PBAT). The filaments were characterized and were used to produce 3D-printed scaffolds using FDM. The scaffolds' structure and surface properties were assessed. In vitro cell, proliferation, and cell mineralization analysis were performed. In vivo data was obtained in the rat femur model (n = 10), and the bone repair was assessed after 15, 30, and 60 postoperative days. The printed structures presented 69.81% porosity for the PBAT/BAGNb group and 74.54% for the PBAT group. Higher cell mineralization was observed for the PBAT/BAGNb group. The in vivo data showed that the PBAT/ BAGNb presented new bone formation comparable to positive controls. The combination of PBAT and BAGNb in 3D-printed scaffolds may be an alternative to produce bioactive materials with controllable shapes and properties for bone regeneration treatments.

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Advances in tissue regeneration through nanomaterials

Vaikkath

Nair

2018

Drug Delivery Nanosystems for Biomedical Applications

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A simple and effective method for making multipotent/multilineage scaffolds with hydrophilic nature without any postmodification/treatment

Cited by 14 publications

References 42 publications

Tissue‐engineered islet‐like cell clusters generated from adipose tissue‐derived stem cells on three‐dimensional electrospun scaffolds can reverse diabetes in an experimental rat model and the role of porosity of scaffolds on cluster differentiation

Tissue‐engineered islet‐like cell clusters generated from adipose tissue‐derived stem cells on three‐dimensional electrospun scaffolds can reverse diabetes in an experimental rat model and the role of porosity of scaffolds on cluster differentiation

3D printing of poly(butylene adipate‐co‐terephthalate) (PBAT)/niobium containing bioactive glasses (BAGNb) scaffolds: Characterization of composites, in vitro bioactivity, and in vivo bone repair

Advances in tissue regeneration through nanomaterials

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