A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues

Möller, E. M.; Bahnweg, G.; Sandermann, Heinrich; Geiger, H. H.

doi:10.1093/nar/20.22.6115

Cited by 1,113 publications

(611 citation statements)

References 4 publications

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“…Strain OWVT-1 was isolated from SCN juvenile in Minnesota, USA and has shown biocontrol potential against nematodes. A single spore isolate of OWVT-1 was cultured on potato dextrose agar (PDA, BD TM , New Jersey, U.S.A.) plate for 4 weeks and the mycelium was harvested for genomic DNA preparation using the CTAB/SDS/Proteinase K method (Möller et al 1992). Whole-genome shotgun sequencing of OWVT-1 was performed using Illumina next generation sequencing technology.…”

Section: Methodsmentioning

confidence: 99%

Introgression and gene family contraction drive the evolution of lifestyle and host shifts of hypocrealean fungi

Zhang

Wang

et al. 2018

Mycology

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Hypocrealean fungi (Ascomycota) are known for their diversity of lifestyles. Their vital influences on agricultural and natural ecosystems have resulted in a number of sequenced genomes, which provide essential data for genomic analysis. Totally, 45 hypocrealean fungal genomes constructed a phylogeny. The phylogeny showed that plant pathogens in Nectriaceae diverged earliest, followed by animal pathogens in Cordycipitaceae, Ophiocordycipitaceae and Clavicipitaceae with mycoparasites in Hypocreaceae. Insect/nematode pathogens and grass endophytes in Clavicipitaceae diverged at last. Gene families associated with host-derived nutrients are significantly contracted in diverged lineages compared with the ancestral species. Introgression was detected in certain lineages of hypocrealean fungi, and the main functions of the genes located in the introgressed regions are involved in host recognition, transcriptional regulation, stress response and cell growth regulation. These results indicate that contraction of gene families and introgression might be main mechanisms to drive lifestyle differentiation and evolution and host shift of hypocrealean fungi.

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Section: Methodsmentioning

confidence: 99%

Introgression and gene family contraction drive the evolution of lifestyle and host shifts of hypocrealean fungi

Zhang

Wang

et al. 2018

Mycology

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“…For DNA sequence-based identification of isolated fungi, cultures were grown at 25 °C and mycelium was scraped from the surface and freeze dried. DNA extractions were done using the method described by Möller et al (1992).…”

Section: Isolation and Dna Extractionmentioning

confidence: 99%

Fungi associated with black mould on baobab trees in southern Africa

Cruywagen

Crous

Roux

et al. 2015

Antonie van Leeuwenhoek

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There have been numerous reports in the scientific and popular literature suggesting that African baobab (Adansonia digitata) trees are dying, with symptoms including a black mould on their bark. The aim of this study was to determine the identity of the fungi causing this black mould and to consider whether they might be affecting the health of trees. The fungi were identified by sequencing directly from mycelium on the infected tissue as well as from cultures on agar. Sequence data for the ITS region of the rDNA resulted in the identification of four fungi including Aureobasidium pullulans, Toxicocladosporium irritans and a new species of Rachicladosporium described here as Rachicladosporium africanum. A single isolate of an unknown Cladosporium sp. was also found. These fungi, referred to here as black mould, are not true sooty mould fungi and they were shown to penetrate below the bark of infected tissue, causing a distinct host reaction. Although infections can lead to dieback of small twigs on severely infected branches, the mould was not found to kill trees.

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“…Mycelium was 158 harvested by scraping the surface of cultures with a sterile scalpel blade and transferring it to 1.5 159 ml Eppendorf tubes. DNA was extracted using the protocol described by Möller et al (1992) with 160 slight modification: samples were not lyophilized before DNA extraction but rather were frozen 161 using liquid nitrogen after the addition of TES buffer (100 mM Tris, pH 8.0, 10 mM EDTA, 2% SDS) 162 and Proteinase K. Furthermore, the samples were not treated with NH 4 Ac but were directly 163 precipitated with 450 µl isopropanol.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Characterization of Phytophthora hybrids from ITS clade 6 associated with riparian ecosystems in South Africa and Australia

et al. 2013

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A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues

Cited by 1,113 publications

References 4 publications

Introgression and gene family contraction drive the evolution of lifestyle and host shifts of hypocrealean fungi

Introgression and gene family contraction drive the evolution of lifestyle and host shifts of hypocrealean fungi

Fungi associated with black mould on baobab trees in southern Africa

Characterization of Phytophthora hybrids from ITS clade 6 associated with riparian ecosystems in South Africa and Australia

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