A Simple and Fast Method for Quantification of Ertapenem using Meropenem as Internal Standard in Human Plasma in a Clinical Setting

Abstract

A high-performance liquid chromatography method for the determination of ertapenem in human plasma using ultraviolet detection (300 nm) was developed and validated. The method involved a simple organic protein precipitation that guarantees rapid sample preparation. By using meropenem as internal standard, it also guarantees improved precision (relative standard deviation <3.70) and accuracy (deviation from true value <6.58%). The method was linear from 0.59 to 150 microg/mL and reproducible. In conclusion, this method allows the measurement of ertapenem in human plasma and may be used for both clinical routine applications and pharmacokinetic studies.

Rapid Detection of Carbapenemase Activity Through Monitoring Ertapenem Hydrolysis in Enterobacteriaceae with LC–MS/MS

“…Several papers have described therapeutic drugmonitoring assays for ertapenem [25][26][27][28] and a similar approach for detection of enzyme-mediated hydrolysis of b-lactam antibiotics using MALDI-TOF instruments, which are currently being used in clinical microbiology laboratories for bacterial future science group identification [18][19][20][21]. We propose that the use of LC-MS/MS described here offers multiple advantages over MALDI-TOF for the detection of carbapenemase activity.…”

Anti-Infective Drugs during Continuous Hemodialysis –Using the Bench to Learn What to do at the Bedside

“…To quantify ceftazidime, ciprofloxacin, flucloxacillin, linezolid, meropenem, metronidazole, piperacillin, rifampicin, and voriconazole, HPLC methods with UV detection were used. All HPLC methods have been previously described (8-10) and were in line with the Valistat 2.0 validation criteria as required by the German Society of Toxicology and Forensic Chemistry (GTFCh); linearity was proven to be within the requested concentration range across all drugs, and quality control samples at 3 different concentrations featured a relative standard deviation (RSD%) for inter- and intraday precision of <10% across all methods. An external standard method was used to quantify drug concentrations, and no protein precipitation was performed for the aqueous solutions.…”

A liquid chromatography assay for a quantification of doripenem, ertapenem, imipenem, meropenem concentrations in human plasma: Application to a clinical pharmacokinetic study

“…Various chromatographic methods with UV detection were developed to measure total plasma concentrations of carbapemens in plasma . These assays concern mainly imipenem [5][6][7][8][9]21], meropenem [10,12,[14][15][16][17][18][19]21] and ertapenem [11,[21][22][23][24][25]. Two assays based on ultrafiltration [20] and solid phase extraction [13] were only reported for doripenem.…”