A simple and high-throughput method for determination and confirmation of 14 coccidiostats in poultry muscle and eggs using liquid chromatography – quadrupole linear ion trap - tandem mass spectrometry (HPLC–QqLIT-MS/MS): Validation according to European Union 2002/657/EC

In this study, a monoclonal antibody with selectivity for clopidol was produced based on a new hapten. The 50% inhibition concentration (IC 50 ) for the developed ic-ELISA was 0.275 ng/mL. Cross-reactivity tests showed that the monoclonal antibody had high specificity for clopidol. Using this antibody, a lateral-flow colloidal gold immunoassay (LCGA) was created for the rapid screening of clopidol in raw chicken samples. With the naked eye, the cut-off value of the strip assay was 5 μg/kg for clopidol in chicken samples. Therefore, this LCGA provides a sensitive and rapid method for on-site detection of clopidol in chicken samples. ARTICLE HISTORY

Section: Sensitivity Of the Strip Assaymentioning

confidence: 99%

Development of a gold nanoparticle-based strip assay for detection of clopidol in the chicken

Chao

Liu

Song

et al. 2020

“…Numerous methods for the determination of diclazuril in feed, animal tissue and eggs have been reported, which included high-performance liquid chromatography with ultraviolet detection (Kock, Smet, & Sneyers, 1992), gas chromatography-mass spectrometry (Blanchflower, Hughes, & Kennedy, 1997), and liquid chromatography-tandem mass spectrometry (Barreto, Ribeiro, Hoff, & Costa, 2017;Mortier, Daeseleire, Huyghebaert, Grijspeerdt, & Peteghem, 2005a;Mortier, Daeseleire, Huyghebaert, Grijspeerdt, & Peteghem, 2005b). But diclazuril (4-chlorophenyl [2,6-dichloro-4-(4,5-dihydro-3H3, 5dioxo-1, 2, 4-triazin-2-yl) phenyl] acetonitrile), is a non-polar compound, the determination of diclazuril using Hydrophilic Interaction Liquid Chromatography (HILIC) goes beyond the typical applications.…”

Section: Introductionmentioning

confidence: 99%

“…But diclazuril (4-chlorophenyl [2,6-dichloro-4-(4,5-dihydro-3H3, 5dioxo-1, 2, 4-triazin-2-yl) phenyl] acetonitrile), is a non-polar compound, the determination of diclazuril using Hydrophilic Interaction Liquid Chromatography (HILIC) goes beyond the typical applications. Although the increased sensitivity and the short retention times have achieved using HILIC-MS/MS (Dasenaki & Thomaidis, 2019) and liquid chromatography-quadrupole linear ion trap-tandem mass spectrometry (HPLC-QTrap-MS/MS) (Barreto et al, 2017), these optimized methods are rather time-consuming, sample pretreatment-complicated and need expensive instruments, thus are not suitable for the screening of a large number of samples. Therefore, there is a great demand for developing simple, inexpensive, high throughput and reliable assay methods to monitor diclazuril in food products.…”

Section: Introductionmentioning

confidence: 99%

High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin–streptavidin system

Wang

et al. 2020

A simple, sensitive and specific chemiluminescence enzyme immunoassay based on biotin-streptavidin system (BASA-CLEIA) for diclazuril was established, which was about 2 and 4 times more sensitive than the traditional chemiluminescence enzyme immunoassay (CLEIA) and enzyme-linked immunosorbent assay. In the optimized generic assay, the half maximal inhibitory concentration (IC 50) of the BASA-CLEIA for diclazuril was 0.48 μg/kg. The limit of detection for diclazuril was 0.02 μg/kg. This method showed no cross reactivity (<0.01%) with toltrazuril, robenidine hydrochloride, nicarbazin, halofuginone, amprolium, monensin, and maduramycin. In chicken muscles, the average recoveries ranged from 90.3% to 120.4%. A further comparison between the developed BSAS-CLEIA and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for real samples showed good correlation (p < 0.01). The results indicated that the simple, sensitive and specific BASA-CLEIA method could be used for the analysis of diclazuril in real samples.

“…Illegal use and failure to comply with specified withdrawal periods before human consumption may result in drug residues in edible animal tissues, posing a potential health risk to consumers. Numerous methods, including high performance liquid chromatography (Amelin & Timofeev, 2016;Barreto, Ribeiro, Hoff, & Dalla Costa, 2017;Clarke et al, 2013), gas chromatography (Ekstrom & Kuivinen, 1984;Newkirk & Barnes, 1989), and liquid chromatography tandem mass spectrometry (Matus & Boison, 2016;Pietruk, Olejnik, Jedziniak, & Szprengier-Juszkiewicz, 2015), have been used for the determination of coccidiostats in milk, eggs, and animal tissue have been reported. Although sensitive and specific, because these methods are rather time-consuming and require complicated sample preparation and expensive instruments, they are unsuitable for screening large numbers of samples (Khaemba et al, 2016;Kong et al, 2017;Liu, Suryoprabowo, Zheng, Song, & Kuang, 2017;Peng, Liu, Kuang, Cui, & Xu, 2017).…”

Section: Introductionmentioning

confidence: 99%

Development of monoclonal antibody-based colloidal gold immunochromatographic assay for analysis of halofuginone in milk

Song

Suryoprabowo

Liu

et al. 2019

A highly sensitive, specific, and rapid immunochromatographic assay for halofuginone (HFG) has been developed. A well-characterized immunogenic conjugate of HFG with bovine serum albumin was produced using the N,N ′′-Carbonyldiimidazole method. A sensitive monoclonal antibody specific for HFG was then generated by immunizing BALB/c mic with the HFG conjugate. The antigen, HFG-CDI-ovalbumin and goat anti-mouse IgG were attached to a nitrocellulose membrane as the test line and the control line, respectively to produce an immunochromatographic test strip for the rapid detection of HFG in milk. Under optimized conditions, the detection limits of the semi-quantitative test strip for HFG were as low as 50 ng/mL in 0.01 M PBS (pH 7.4) and 100 ng/mL in milk. All the results were obtained within 5 min. The newly developed immunochromatographic assay provides a sensitive, rapid, and specific procedure for on-site screening for HFG.