A simple and highly stable free-flow electrophoresis device with thermoelectric cooling system

Yan, Jian; Guo, Cheng-Gang; Liu, Xiaoping; Kong, Fan-Zhi; Shen, Qiao-Yi; Yang, Cheng‐Zhang; Li, Jun; Cao, Cheng-Xi; Jin, Xinqiao

doi:10.1016/j.chroma.2013.10.058

Cited by 16 publications

(24 citation statements)

References 39 publications

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“…Temperature control has been utilized in a number of microfluidic applications, including temperature gradient focusing [30,31], isoelectric focusing [32], free flow electrophoresis [33], and zone electrophoresis [34]. Temperature control on microfluidic devices has been performed in a variety of manners [35], from copper blocks [30,31] to Peltier elements [33] to channels carrying temperature-controlling fluids integrated next to features of interest [36].…”

Section: Resultsmentioning

confidence: 99%

“…Temperature control on microfluidic devices has been performed in a variety of manners [35], from copper blocks [30,31] to Peltier elements [33] to channels carrying temperature-controlling fluids integrated next to features of interest [36]. While much work has gone into implementing temperature control methods for these assays, little has been done on implementing methods for optimization of affinity separations in microfluidic separations even though the affinity complex shows a strong dependence on temperature [21–23].…”

Section: Resultsmentioning

confidence: 99%

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Optimization of a microfluidic electrophoretic immunoassay using a Peltier cooler

Mukhitov

Schrell

et al. 2014

Journal of Chromatography A

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Successful analysis of electrophoretic affinity assays depends strongly on the preservation of the affinity complex during separations. Elevated separation temperatures due to Joule heating promotes complex dissociation leading to a reduction in sensitivity. Affinity assays performed in glass microfluidic devices may be especially prone to this problem due to poor heat dissipation due to the low thermal conductivity of glass and the large amount of bulk material surrounding separation channels. To address this limitation, a method to cool a glass microfluidic chip for performing an affinity assay for insulin was achieved by a Peltier cooler localized over the separation channel. The Peltier cooler allowed for rapid stabilization of temperatures, with 21 °C the lowest temperature that was possible to use without producing detrimental thermal gradients throughout the device. The introduction of cooling improved the preservation of the affinity complex, with even passive cooling of the separation channel improving the amount of complex observed by 2-fold. Additionally, the capability to thermostabilize the separation channel allowed for utilization of higher separation voltages than what was possible without temperature control. Kinetic CE analysis was utilized as a diagnostic of the affinity assay and indicated that optimal conditions were at the highest separation voltage, 6 kV, and the lowest separation temperature, 21 °C, leading to 3.4% dissociation of the complex peak during the separation. These optimum conditions were used to generate a calibration curve and produced 1 nM limits of detection, representing a 10-fold improvement over non-thermostated conditions. This methodology of cooling glass microfluidic devices for performing robust and high sensitivity affinity assays on microfluidic systems should be amenable in a number of applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Optimization of a microfluidic electrophoretic immunoassay using a Peltier cooler

Mukhitov

Schrell

et al. 2014

Journal of Chromatography A

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“…Arguably, the most successful implementation of thermoelectric cooling for steady-state purification was developed last year by Cao et al [51]. They designed a mid-scale FFE device with a ceramic bottom surface, a material with high thermal conduction.…”

Section: Joule Heatingmentioning

confidence: 99%

Advances in steady-state continuous-flow purification by small-scale free-flow electrophoresis

Agostino

Krylov

2015

TrAC Trends in Analytical Chemistry

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“…During the last five years, an alternative FFE system with a gravity‐induced self‐balance sample collector has been developed for reducing the cost of the current FFE device. One of the features in the newly developed FFE system was that the gravity‐induced pressure coupled to a single pump was used for self‐balance fraction collection and uniform flow of BGE in the separation chamber, for displacing the multichannel pump used in the highly advanced FFE device.…”

Section: Introductionmentioning

confidence: 99%

“…One of the features in the newly developed FFE system was that the gravity‐induced pressure coupled to a single pump was used for self‐balance fraction collection and uniform flow of BGE in the separation chamber, for displacing the multichannel pump used in the highly advanced FFE device. The newly developed FFE system has been used for the separation of amino acids, proteins, drugs, and cells .…”

Section: Introductionmentioning

confidence: 99%

Negative‐pressure‐induced collector for a self‐balance free‐flow electrophoresis device

Yang

Yan

Zhang

et al. 2014

J of Separation Science

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Uneven flow in free-flow electrophoresis (FFE) with a gravity-induced fraction collector caused by air bubbles in outlets and/or imbalance of the surface tension of collecting tubes would result in a poor separation. To solve these issues, this work describes a novel collector for FFE. The collector is composed of a self-balance unit, multisoft pipe flow controller, fraction collector, and vacuum pump. A negative pressure induced continuous air flow rapidly flowed through the self-balance unit, taking the background electrolyte and samples into the fraction collector. The developed collector has the following advantages: (i) supplying a stable and harmonious hydrodynamic environment in the separation chamber for FFE separation, (ii) effectively preventing background electrolyte and sample flow-back at the outlet of the chamber and improving the resolution, (iii) increasing the preparative scale of the separation, and (iv) simplifying the operation. In addition, the cost of the FFE device was reduced without using a multichannel peristaltic pump for sample collection. Finally, comparative FFE experiments on dyes, proteins, and cells were carried out. It is evident that the new developed collector could overcome the problems inherent in the previous gravity-induced self-balance collector.

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A simple and highly stable free-flow electrophoresis device with thermoelectric cooling system

Cited by 16 publications

References 39 publications

Optimization of a microfluidic electrophoretic immunoassay using a Peltier cooler

Optimization of a microfluidic electrophoretic immunoassay using a Peltier cooler

Advances in steady-state continuous-flow purification by small-scale free-flow electrophoresis

Negative‐pressure‐induced collector for a self‐balance free‐flow electrophoresis device

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