A simple and rapid high performance liquid chromatographic method with fluorescence detection for the estimation of fexofenadine in rat plasma—Application to preclinical pharmacokinetics

Pathak, Shriram M.; Kumar, Ranjith A. Ashok; Musmade, Prashant B; Udupa, N.

doi:10.1016/j.talanta.2008.02.047

Cited by 28 publications

(31 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…12 The method is selective if there is no interfering peak present at the retention time of the drug or IS. If there is any interfering peak present at the retention time of drug then its response should be less than 20% of mean response of six extracted LOQ-QC samples.…”

Section: Selectivitymentioning

confidence: 99%

“…Where, H is the height of the peak in a chromatogram obtained with the prescribed reference solution and h is the noise in blank chromatogram. 12 The limit of quantification (LLOQ) was defined as the lowest concentration with ratio of signal-to-noise more than 5 with accuracy of 80e120% and precision of 20% to its nominal value.…”

Section: Detection and Quantification Limitmentioning

confidence: 99%

See 1 more Smart Citation

Development and validation of RP-HPLC method with ultraviolet detection for estimation of montelukast in rabbit plasma: Application to preclinical pharmacokinetics

Ranjan¹,

Nayak²,

Reddy³

et al. 2013

Journal of Young Pharmacists

View full text Add to dashboard Cite

a b s t r a c tObjective: To develop a liquid-liquid extraction based reverse phase liquid chromatography method for estimation of montelukast in rabbit plasma. Methods: Chromatographic separation was carried out using Phenomenex Luna C18 column (250 mm Â 4.6 mm Â 5 mm) with mobile phase composed of ammonium acetate buffer (20 Mm), pH 5.5 and acetonitrile in 20:80, v/v ratio. The analyte was monitored with UV detector at 345 nm. The developed method was validated with respect to linearity, accuracy, precision, specificity and stability. Results: The peak area ratio of montelukast (MKS) to that of internal standard was used for the quantification of samples. Calibration curves were linear in the concentration range of 20e2000 ng mL À1. The LOD and LLOQ of present method were found out to be 10 ng mL À1 and 20 ng mL À1 respectively. The intra-day and inter-day %CV values for MKS were below 6.06% and 8.43%. Intra-day and inter-day accuracies were within 95.81% and 110.90%, respectively. Extraction recoveries of drug from rabbit plasma were >66.47%. Conclusion: A simple, alternative, reproducible and sensitive HPLC-UV method was developed for MKS that can be used in preclinical pharmacokinetics.

show abstract

Section: Selectivitymentioning

confidence: 99%

Section: Detection and Quantification Limitmentioning

confidence: 99%

Development and validation of RP-HPLC method with ultraviolet detection for estimation of montelukast in rabbit plasma: Application to preclinical pharmacokinetics

Ranjan¹,

Nayak²,

Reddy³

et al. 2013

Journal of Young Pharmacists

View full text Add to dashboard Cite

show abstract

“…29) Consequently, we investigated various mobile phases: 0.1% formic acid (pH 2.7); 0.1% acetic acid (pH 3.2); 10 mM ammonium formate (pH 5.7); 10 mM ammonium acetate (pH 6.4); and 10 mM ammonium hydrogen carbonate (pH 7.8). The highest peak intensity and a good peak shape were obtained with fexofenadine when the 0.1% formic acid mobile phase was used (data not shown).…”

Section: Fig 4 Representative Chromatograms Of the Calibration Curvmentioning

confidence: 99%

Development of a Highly Sensitive Methodology for Quantitative Determination of Fexofenadine in a Microdose Study by Multiple Injection Method Using Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry

Tanaka

Yoshikawa

Yasui

2012

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

An ultra high-sensitivity method for quantifying fexofenadine concentration in rat plasma samples by multiple injection method (MIM) was developed for a microdose study. In this study, MIM involved continuous injections of multiple samples containing the single compound into a column of the ultra-HPLC (UHPLC) system, and then, temporary trapping of the analyte at the column head. This was followed by elution of the compound from the column and detection by mass spectrometer. Fexofenadine, used as a model compound in this study, was extracted from the plasma samples by a protein precipitation method. Chromatographic separation was achieved on a reversed-phase C18 column by using a gradient method with 0.1% formic acid and 0.1% formic acid in acetonitrile as the mobile phase. The analyte was quantified in the positive-ion electrospray ionization mode using selected reaction monitoring. In this study, the analytical time per fexofenadine sample was approximately 2 min according to the UHPLC system. The method exhibited the linear dynamic ranges of 5-5000 pg/mL for fexofenadine in rat plasma. The intra-day precisions were from 3.2 to 8.7% and the accuracy range was 95.2-99.3%. The inter-day precisions and accuracies ranged from 3.5 to 8.4% and from 98.6 to 102.6%, respectively. The validated MIM was successfully applied to a microdose study in the rats that received oral administration of 100 µg/kg fexofenadine. We suggest that this method might be beneficial for the quantification of fexofenadine concentrations in a microdose clinical study.

show abstract

“…Other non-specifi c HPLC detection systems such as the evaporative light scattering detector (Malm and Bergqvist, 2007) and fl uorescence detector (Pathak et al, 2008) have also been applied to the determination of target analytes in biological fl uids. Although possible in certain cases, these non-specifi c detection methods are generally not suitable for this purpose, especially when used to detect the metabolites of these analytes, which have even lower concentrations.…”

Section: Uv Detectionmentioning

confidence: 99%

Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids: a review

Gray

Chang

Zhang

et al. 2009

Biomedical Chromatography

View full text Add to dashboard Cite

The development of liquid chromatography-mass spectrometry (LC-MS) and tandem MS/MS for the analysis of bioactive components and their metabolites of herbal medicines in biological fluids is reviewed with the aim of providing an overview of the current techniques and methods used. The issues and challenges associated with various stages of the analytical method development are discussed using Ginkgo biloba and Panax ginseng as case studies. LC-MS offers selectivity and specificity in both the chromatographic separation and detection steps. This is necessary in order to measure compounds at extremely low concentrations as is often observed in plasma and urine samples. Traditional methods of detection (UV-visible) do not offer sufficient selectivity and specificity needed. The strategies and pitfalls involved with the measurement of such compounds are discussed in this review. Matrix effects, 'unseen' matrix suppression and enhancement ionization effects can significantly reduce the accuracy and precision of the measurement. The impact of the correct choice of chromatography column formats on signal-to-noise ratio is also discussed. Analytical methods from sample preparation to mass spectrometric detection is outlined in order to provide good direction for analysts intent on the measurement of bioavailable compounds from herbal medicines in plasma and urine samples.

show abstract

A simple and rapid high performance liquid chromatographic method with fluorescence detection for the estimation of fexofenadine in rat plasma—Application to preclinical pharmacokinetics

Cited by 28 publications

References 9 publications

Development and validation of RP-HPLC method with ultraviolet detection for estimation of montelukast in rabbit plasma: Application to preclinical pharmacokinetics

Development and validation of RP-HPLC method with ultraviolet detection for estimation of montelukast in rabbit plasma: Application to preclinical pharmacokinetics

Development of a Highly Sensitive Methodology for Quantitative Determination of Fexofenadine in a Microdose Study by Multiple Injection Method Using Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry

Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids: a review

Contact Info

Product

Resources

About