A simple and rapid HPLC method for determination of S-allyl-L-cystein and its use in quality control of black garlic samples

Improving endothelial health with food-derived H2S donors: an in vitro study with S-allyl cysteine and with a black-garlic extract enriched in sulfur-containing compounds

“…The scientific literature reports several methods for the quantitation of S-compounds: an application for the S-allyl cysteine quantitative study has been developed with a HPLC-FLD and HPLC-UV detector; in this case, for the poor response of SAC with spectrophotometric detectors a derivatization step is strongly required. [38][39][40] The use of a MS detector overcomes this limitation and allows the analysis to reach a higher sensitivity; an interesting HPLC-MS method was validated for the quantitation of SAC in heated garlic juice with similar conditions to ours; 41 the paper presents an interesting comparison between UV and MS detector and highlights the sensitivity differences between the two techniques. For our method, the optimal chromatographic conditions were found using a Luna C18 (2) column with an organic gradient of 0.1% formic acid in ACN starting from 5% in 0.1% formic acid in water.…”

Effect of Thermal Processes on S-Allyl Cysteine Content in Black Garlic

“…SAC was analyzed at the Department of Chemistry, Faculty of Science, Burapha University, by high-performance liquid chromatography using Agilent 1260 Infinity II (Ag Technologies, Inc., CA, USA) with photodiode array detector (DAD, λ = 250 nm, injection volume = 10 µL) according to the method described by Malapong et al [ 29 ]; 1 g of chopped BG in 15 mL of distilled water was derivatized with dansyl chloride at room temperature for 15 min at a mobile phase of 45% ( w / v ) sodium acetate buffer, pH 5, and 55% ( v / v ) methanol in a Poroshell C-18 column. The amount of SAC was calculated from the peak area of the chromatography [ 29 ].…”

The nutritional value, bioactive availability and functional properties of garlic and its related products during processing

“…It can penetrate tissues, including those of the liver, kidneys, heart, and brain ( 38 ). The studies indicate that SAC may be degraded by cysteine dioxygenase and glutamyl transpeptidase, leading to the formation of cysteine and other metabolites that may contribute to its biological activities ( 39 , 40 ).…”