A simple and rapid HPLC–MS method for the simultaneous determination of epinephrine, norepinephrine, dopamine and 5-hydroxytryptamine: Application to the secretion of bovine chromaffin cell cultures

Carrera, Victoria; Sabater, Esther; Vilanova, Eugenio; Sogorb, Miguel A.

doi:10.1016/j.jchromb.2006.09.032

Cited by 438 publications

(184 citation statements)

References 19 publications

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“…When we compare the LODs of the present method with those of the HPLC-MS method without derivatization, the present method revealed 32.5 times better sensitivity with DA and 3.9 times better sensitivity with 5-HT than HPLC-MS without derivatization since the detection limits of DA and 5-HT by HPLC-MS method without derivatization were 6.5 ng/mL and 0.78 ng/mL, respectively. 17) Upon comparison of the present method with the HPLC-FLD method with derivitization, it was found that the LODs of DA and 5-HT using HPLC-FLD method have approximately 67 times better sensitivity with DA and 38 times better sensitivity with 5-HT than those using the present method. However, the HPLC-FLD method requires the vigorous reaction conditions of heating to 50°C for 20 min for the derivatization, which are not desirable reaction conditions for biological samples.…”

Section: Application Of the Methods To Real Biological Samplesmentioning

confidence: 78%

“…HPLC coupled with MS, which can be used to identify analytes simply on the basis of their m/z (mass-to-charge) ratios, 15,16) is a popular and powerful technique for analyzing neurotransmitters in several biological fluids. 17) However, it was to our interest that there was no report for the simultaneous determination of neurotransmitters containing different ionizable groups such as carboxylic acid or amino groups in the biological samples by using the HPLC-MS method. Thus, in order to determine a neurotransmitter containing an amino group, we have to use a positive ion polarity mode of MS condition while using a negative ion polarity mode of MS condition for determining a neurotransmitter containing a carboxylic acid group.…”

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confidence: 99%

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Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Park

Myung

Kim

et al. 2013

Biological & Pharmaceutical Bulletin

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In order to measure the levels of serotonin (5-hydroxyltryptamine, 5-HT), dopamine (DA), 3,4-hydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) simultaneously, an effective derivatization method followed by high-performance liquid chromatography (HPLC) coupled to electrochemical ionization mass spectrometry was used. The derivatization reaction of biological samples with ethyl chloroformate occurred rapidly at room temperature in aqueous conditions, and the resulting derivatives were isocratically separated with good selectivity using a C18 reversed-phase column within 30 min. The study results showed that the new derivatization procedure offers an excellent means of simultaneous determination of 5-HT, DA and their metabolites in mouse brain homogenates, which are important in a number of physiological and behavioral functions.Key words neurotransmitter; HPLC-MS; ethyl chloroformate; derivatization Dopamine (DA) and serotonin (5-hydroxyltryptamine, 5-HT), which are widely distributed as a monoamine neurotransmitter in the central nervous system (CNS), 1) play important physiological and behavioral roles such as the control and regulation of affective behavior, sleep, reward system, motor and cognitive functions.2) Changes of DA and 5-HT basal levels have been linked to some CNS-related disorders such as depression, panic disorder, schizophrenia and Parkinson's disease (PD).2,3) PD is pathologically characterized by the death of dopaminergic neurons in the substantia nigra pars compacta 4) and caused by neurotoxins such as 1-methyl-4-phenyl-1,2,3,6-tetrahypyridine (MPTP), which produces a clinical syndrome similar to PD. Because the administration of MPTP is known to affect the dopaminergic neurons in the nigrostriatal system with subsequent depletion of DA and its metabolites, 5,6) the measurement of 5-HT, DA and their metabolites is necessary to discover and test new drugs for treating these disorders. 7)Various analytical methods have been widely investigated for determining the level of monoamine neurotransmitters and their metabolites, including high-performance liquid chromatography (HPLC) coupled with electrochemical detection (ECD), dual ECD, fluorescence detection (FLD), FLD/ECD system, chemiluminescence detection and mass spectrometer (MS) and gas chromatography (GC) coupled with MS in biological samples such as plasma, rat brain, and mouse brain and in vitro experiments.8-10) HPLC-ECD, which is the most common analytical technique used to determine monoamines and their metabolites, has the advantages of low cost and fast speed 11) but maintaining its routine use is problematic due to sudden noise problems.12) The HPLC-FLD method is used to measure fluorescent derivatives of monoamine neurotransmitters by a derivatization procedure, 13) which was performed by benzylamine and 1,2-diphenylethylenediamine in a two-step reaction in weakly alkaline media and in the presence of potassium hexacyanoferrate (III).14) However, since this protocol requires the vigorous reaction ...

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Section: Application Of the Methods To Real Biological Samplesmentioning

confidence: 78%

mentioning

confidence: 99%

Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Park

Myung

Kim

et al. 2013

Biological & Pharmaceutical Bulletin

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“…Together with low cost, rapidness, and simplicity, the MnO 2 NWs-RGO/GCEs are expected to detect dopamine in various real samples. 6 ]), sodium dihydrogen phosphate (NaH 2 PO 4 ), disodium hydrogen phosphate (Na 2 HPO 4 ), sodium hydroxide (NaOH), and ethyl alcohol were analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dopamine was purchased from Sigma-Aldrich Co (St. Louis, CA, USA).…”

Section: Electrochemical Sensing Perfomances Of Mno 2 Nws-rgo/gcementioning

confidence: 99%

“…Therefore, it is of great significance to detect dopamine accurately at a physiological level in the earlier prevention and clinical diagnosis of these neurological diseases. To date, various techniques have been developed to detect dopamine, including but not limited to high performance liquid chromatography [6], mass spectrometry, fluorescent spectrometry [7], capillary electrophoresis [8,9], and electrochemiluminescence [10,11]. Despite being reliable and precise, these methods often suffer from drawbacks such as expensive instruments, complex and time-consuming analytical procedures, and the requirement of experienced technical staff.…”

Section: Introductionmentioning

confidence: 99%

Morphologically Tunable MnO2 Nanoparticles Fabrication, Modelling and Their Influences on Electrochemical Sensing Performance toward Dopamine

Liu

et al. 2018

Catalysts

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Abstract:The morphology or shape of nanomaterials plays an important role in functional applications, especially in the electrochemical sensing performance of nanocomposites modified electrodes. Herein, the morphology-dependent electrochemical sensing properties of MnO 2 -reduced graphene oxide/glass carbon electrode (MnO 2 -RGO/GCE) toward dopamine detection were investigated. Firstly, various morphologies of nanoscale MnO 2 , including MnO 2 nanowires (MnO 2 NWs), MnO 2 nanorods (MnO 2 NRs), and MnO 2 nanotubes (MnO 2 NTs), were synthesized under different hydrothermal conditions. Then the corresponding MnO 2 -RGO/GCEs were fabricated via drop-casting and the subsequent electrochemical reduction method. The oxidation peak currents increase with the electrochemical activity area following the order of MnO 2 NWs-RGO/GCE, MnO 2 NTs-RGO/GCE, and MnO 2 NRs-RGO/GCE. The spatial models for MnO 2 NWs, MnO 2 NTs, and MnO 2 NRs are established and accordingly compared by their specific surface area, explaining well the evident difference in electrochemical responses. Therefore, the MnO 2 NWs-RGO/GCE is selected for dopamine detection due to its better electrochemical sensing performance. The response peak current is found to be linear with dopamine concentration in the range of 8.0 × 10 −8 mol/L-1.0 × 10 −6 mol/L and 1.0 × 10 −6 mol/L-8.0 × 10 −5 mol/L with a lower detection limit of 1 × 10 −9 mol/L (S/N = 3). Finally, MnO 2 NWs-RGO/GCE is successfully used for the determination of dopamine injection samples, with a recovery of 99.6-103%. These findings are of great significance for understanding the relationship between unlimited nanoparticle structure manipulation and performance improvement.

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“…It is for this reason that DA has been long used as an important biomarker in the diagnosis of these neural diseases. Electrochemical methods [27] [28], capillary electrophoresis [29] [30], and liquid chromatography-mass spectrometry [31] are conventional analytical methods for the detection of DA. However, these methods are time consuming and require expensive instruments.…”

Section: Introductionmentioning

confidence: 99%

Templated J-Aggregate Nanotubes for the Detection of Dopamine

Rhodes¹,

Wang²,

Liang³

et al. 2017

MSCE

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J-aggregates of dye molecules are a unique supramolecular structure, which shows great promise in photoelectric devices due to its remarkable optical and transport properties. In this paper, we report the templated formation of J-aggregate nanotubes by the adsorption of 3,3'-diethylthiacarbocyanine iodide on the self-assembled nanotubes of lithocholic acid. The optical and electronic properties of the templated J-aggregate nanotubes are studied. A sensor platform is fabricated by depositing the J-aggregate nanotubes on interdigitated gold electrodes for the detection of dopamine (DA). We find that the current change of the J-aggregate nanotube-based sensor platform in response to DA is linear in the concentration range from 10 nM to 70 nM, giving the detection limit of 0.27 nM.

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A simple and rapid HPLC–MS method for the simultaneous determination of epinephrine, norepinephrine, dopamine and 5-hydroxytryptamine: Application to the secretion of bovine chromaffin cell cultures

Cited by 438 publications

References 19 publications

Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Morphologically Tunable MnO2 Nanoparticles Fabrication, Modelling and Their Influences on Electrochemical Sensing Performance toward Dopamine

Templated J-Aggregate Nanotubes for the Detection of Dopamine

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