A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification

Yang, Jing; Yang, Ruifu; Cheng, Anchun; Wang, Mingshu; Fu, Lizhi; Song-quan, Yang; Zhang, Su-Hui; Liu, Yang; Xu, Zhiyong

doi:10.1186/1743-422x-7-14

Cited by 33 publications

(20 citation statements)

References 18 publications

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“…in a water bath. Similar results were obtained by JinLong et al (2) regarding the application of the LAMP method for the detection of goose parvovirus infections.…”

Section: Discussionsupporting

confidence: 88%

“…The most serious threat to goslings and adult geese are viral diseases, among which are Derzsy's disease (DD) caused by goose parvovirus (GPV), haemorrhagic nephritis and enteritis syndrome of geese (HNEG), the aetiological factor of which is goose haemorrhagic polyomavirus (GHPV), and goose circovirus infections (GoCV) (1)(2)(3)(4)6). The correct diagnosis and differentiation of these three diseases on the basis of clinical symptoms and anatomopathological changes are difficult, and sometimes even impossible, and therefore they must be confirmed in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Elaboration of triplex PCR for detection of selected viral infections in waterfowl

Kozdrun

Czekaj

Styś-Fijoł

et al. 2019

Journal of Veterinary Research

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IntroductionViral infections are the greatest threat to waterfowl and cause significant economic losses. Diagnosis and differentiation of three goose viruses is difficult in the field and often requires laboratory confirmation. Therefore, the aim of the study was to develop a triplex PCR and optimise its parameters for simultaneous detection of DNA of goose parvovirus (GPV), goose polyomavirus (GHPV), and goose circovirus (GoCV).Material and MethodsThe DNA of viruses isolated from field cases from the National Veterinary Research Institute’s own collection was used for the study. The primer attachment temperature, the number of reaction cycles, and the Taq DNA polymerase and Mg2+ concentrations were optimised. The sensitivity and specificity of this triplex PCR was also determined.ResultsBased on the obtained results, triplex PCR parameters were optimised for simultaneous detection of DNA of GPV, GHPV, and GoCV in one sample. The following PCR products of the expected size were obtained: GPV DNA of 806 bp, GoCV DNA of 571 bp, and GHPV DNA of 180 bp.ConclusionThe developed triplex PCR method proved to be useful for simultaneous detection of infections with three waterfowl viruses and will be used in relevant laboratory diagnostics.

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“…in a water bath. Similar results were obtained by JinLong et al (2) regarding the application of the LAMP method for the detection of goose parvovirus infections.…”

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Elaboration of triplex PCR for detection of selected viral infections in waterfowl

Kozdrun

Czekaj

Styś-Fijoł

et al. 2019

Journal of Veterinary Research

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“…However, use of real-time PCR assay is limited because of cost of instrumentation, cost of enzyme mixes sold by the machine manufacturers and the cost of reagents especially fluorogenic probe that is more expensive than the regular primers (Curry et al 2002). JinLong et al (2010) analysed LAMP and fluorescent quantitative real-time PCR (FQ-PCR) assay for goose parvovirus infected gosling tissues. Twenty one of the 30 samples were tested positive, while nine were negative, based on FQ-PCR and LAMP.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of loop-mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens

Mukhopadhyay¹,

Amsaveni²,

Matta³

et al. 2012

Letters in Applied Microbiology

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Aims: To develop a specific and highly sensitive loop‐mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. Methods and Results: LAMP reaction was developed by designing two sets of outer and inner primers, which target a total of six distinct regions on VP2 gene of CPV. The template DNA was prepared by a simple boiling and chilling method. Of the 140 faecal samples screened by the developed LAMP and the conventional PCR assays, 104 samples (74·28%) were found positive by LAMP, whereas 81 samples (57·85%) were found positive by PCR. The specificity of the LAMP assay was tested by cross‐examination of common pathogens of dogs and further confirmed by sequencing. The detection limit of the LAMP was 0·0001 TCID50 ml−1, whereas the detection limit of the PCR was 1000 TCID50 ml−1. Conclusions: The developed LAMP assay detects CPV DNA in faecal specimens directly within an hour by following a simple and rapid boiling and chilling method of template preparation. The result also shows that the developed LAMP assay is specific and highly sensitive in detecting CPV. Significance and Impact of the Study: The result indicates the potential usefulness of LAMP which is a simple, rapid, specific, highly sensitive and cost‐effective field‐based method for direct detection of CPV from the suspected faecal samples of dogs.

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“…According to the previous study of LAMP assays (Kuboki et al, 2003;Parida et al, 2008), typical thymine spacers were used to enhance LAMP efficiency by assisting the loop formation. Hwang et al (Yang et al, 2010) investigated the effects of LAMP amplification associated by inserting T-bases into the FIP and BIP, which suggested that the LAMP primer containing T4 linker sequences would be greater than the conventional PCR method. However, in our experiment, the T4 linker didn't seem to affect gene amplification, and its use was rather a failure in LAMP reaction.…”

Section: Lamp Target and Primer Efficiencymentioning

confidence: 99%

One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV

et al. 2017

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Due to the limitation of rapid development of specific antiviral drug or vaccine for novel emerging viruses, an accurate and rapid diagnosis is a key to manage the virus spread. We developed an efficient and rapid method with high specificity for the Middle East Respiratory Syndrome coronavirus (MERS-CoV), based on one-pot reverse transcription loop-mediated isothermal amplification (one-pot RT-LAMP). A set of six LAMP primers [F3, B3, FIP, BIP, LF (Loop-F), and LB (Loop-B)] were designed using the sequence of nucleocapsid (N) gene with optimized RT-LAMP enzyme conditions: 100 U M-MLV RTase and 4 U Bst polymerase, implying that the reaction was able to detect four infectious viral genome copies of MERS-CoV within a 60 min reaction time period. Significantly, EvaGreen dye has better signal read-out properties in one-pot RT-LAMP reaction and is more compatible with DNA polymerase than SYBR green I. Isothermally amplified specific N genes were further evaluated using field-deployable microchamber devices, leading to the specific identification of as few as 0.4 infectious viral genome copies, with no cross-reaction to the other acute respiratory disease viruses, including influenza type A (H1N1 and H3N2), type B, human coronavirus 229E, and human metapneumovirus. This sensitive, specific and feasible method provides a large-scale technical support in emergencies, and is also applied as a sample-to-detection module in Point of Care Testing devices.

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A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification

Cited by 33 publications

References 18 publications

Elaboration of triplex PCR for detection of selected viral infections in waterfowl

Elaboration of triplex PCR for detection of selected viral infections in waterfowl

Development and evaluation of loop-mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens

One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV

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