A Simple and Rapid Method for DNA Isolation from Xylophagous Insects

Calderón-Cortés, Nancy; Quesada, Maurício; Cano-Camacho, Horacio; Zavala-Páramo, María Guadalupe

doi:10.3390/ijms11125056

Cited by 48 publications

(33 citation statements)

References 26 publications

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“…Some are pests on forest and their product in forest and timber industry, due to their economic and ecological role research conducted on control of these insect, by study systematic, ecological, phylogenetic and molecular genetics. For all these research, high quality DNA is extracted [2].…”

Section: Introductionmentioning

confidence: 99%

DNA Extraction from Insects by Using Different Techniques: A Review

Asghar¹,

Malik²,

Anwar³

et al. 2015

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The data for the two decades, DNA Extraction from Insects by Using Different Techniques was compiled through a thorough review of many research articles published in various journals of international repute. Insects are very important economically and ecologically for human due to their various roles, as pests, decomposer in nutrition cycle, vector for the transmission of certain diseases and a source of medically and economically important products. To identify insect, and for research on their role, different molecular techniques are used. For each molecular technique, high quality DNA is required, which is extracted from the insects by different techniques. The selection of DNA extraction technique is depended upon specimen under study, time required for extraction, economical stander of technique due to reagents and equipment used for extraction and most importantly extracted DNA quality. In this paper, most frequently used techniques for DNA extraction from insect with different size and condition are outlined. This paper is only a guide for these techniques and we describe them briefly.

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Section: Introductionmentioning

confidence: 99%

DNA Extraction from Insects by Using Different Techniques: A Review

Asghar¹,

Malik²,

Anwar³

et al. 2015

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show abstract

“…Experiments were repeated three times. Total DNA was isolated from whiteflies according to(Calderón-Cortés et al, 2010). Semi-quantitative PCR was carried out according toBonfim et al, (2007) using the primer pair AC3F (3´) to amplify a 223-bp sequence from the Replication Enhancer (REn) gene of BGMV.…”

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confidence: 99%

Reduction of viral load in whitefly (Bemisia tabaci Gen.) feeding on RNAi-mediated bean golden mosaic virus resistant transgenic bean plants

2015

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“…Various molecular protocols have been developed, but most of them should be optimized for specific specimens (Maddison et al, 1999;Calderon-Cortes et al, 2010;Kotze et al, 2011).…”

Section: Introductionmentioning

confidence: 99%