A simple and reliable method for discriminating between Helicoverpa armigera and Helicoverpa assulta (Lepidoptera: Noctuidae)

Chen, Jia; Wu, Yuchun; Chen, Xin; Ji, Ya-Jie; Zhang, Dexing

doi:10.1111/j.1744-7917.2011.01413.x

Cited by 5 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, they are commonly used as research materials in the field of entomology, boasting a substantial foundation of scientific studies. Morphologically, the two species are nearly indistinguishable at all stages, including the egg, larva, and pupal stages, and only identifiable during the adult stage by certain characteristics 2 , 3 . Physiologically, they have the same major sex pheromone components of (Z)-9-hexadecenal and (Z)-11-hexadecenal 4 .…”

Section: Background and Summarymentioning

confidence: 99%

A chromosome-level haplotype-resolved genome assembly of oriental tobacco budworm (Helicoverpa assulta)

Xu,

Wang,

et al. 2024

Sci Data

View full text Add to dashboard Cite

Oriental tobacco budworm (Helicoverpa assulta) and cotton bollworm (Helicoverpa armigera) are two closely related species within the genus Helicoverpa. They have similar appearances and consistent damage patterns, often leading to confusion. However, the cotton bollworm is a typical polyphagous insect, while the oriental tobacco budworm belongs to the oligophagous insects. In this study, we used Nanopore, PacBio, and Illumina platforms to sequence the genome of H. assulta and used Hifiasm to create a haplotype-resolved draft genome. The Hi-C technique helped anchor 33 primary contigs to 32 chromosomes, including two sex chromosomes, Z and W. The final primary haploid genome assembly was approximately 415.19 Mb in length. BUSCO analysis revealed a high degree of completeness, with 99.0% gene coverage in this genome assembly. The repeat sequences constituted 38.39% of the genome assembly, and we annotated 17093 protein-coding genes. The high-quality genome assembly of the oriental tobacco budworm serves as a valuable genetic resource that enhances our comprehension of how they select hosts in a complex odour environment. It will also aid in developing an effective control policy.

show abstract

Section: Background and Summarymentioning

confidence: 99%

A chromosome-level haplotype-resolved genome assembly of oriental tobacco budworm (Helicoverpa assulta)

Xu,

Wang,

et al. 2024

Sci Data

View full text Add to dashboard Cite

show abstract

“…The oriental tobacco budworm, H. assulta, is also considered a sibling species with H. armigera , being morphologically very similar and almost indistinguishable at the egg, larval and pupal stage (Chen et al, 2011; Ming & Wang, 2006). H. armigera and H. assulta are considered as sympatric and damaging crop pests in China and other countries (Fitt, 1989).…”

Section: Introductionmentioning

confidence: 99%

A Real‐time PCR assay for rapid identification of Helicoverpa armigera (Lepidoptera: Noctuidae) from closely related species

Thomas

Gunawardana

et al. 2021

J Applied Entomology

View full text Add to dashboard Cite

The bollworm, Helicoverpa armigera, is one of the most important agricultural pests in the world. Diagnosis of this species morphologically is difficult from the similar species H. assulta, H. punctigera and H. zea as the adults are identified based on genitalia morphology and larval identification is limited due to shared morphological characters. To provide an accurate diagnosis of New Zealand border interceptions, we have developed a real‐time PCR assay to identify H. armigera from commonly occurring exotic Helicoverpa species, H. assulta and H. zea. Preliminary studies, using the already available real‐time ITS2 primer–probe detecting H. armigera and H. zea tested on samples obtained from NZ border interceptions and from overseas showed cross reactions with low intensities among H. armigera, H. assulta and H. zea. This prompted PHEL to develop a new real‐time PCR assay to accurately identify H. armigera. The newly designed primer and probe were optimized, and the assay demonstrated its specificity to H. armigera with no false positives observed in the non‐target species tested in this study. The specificity of the assay was tested against 64 samples of Lepidoptera from 14 countries including 48 Helicoverpa samples. The sensitivity of the assay showed it can detect up to 1 copy/µl of the DNA fragment. Therefore, this assay can be used for rapid detection of H. armigera, which is imperative in quarantine application to determine the presence of exotic species regardless of its life stage.

show abstract

Absence of known knockdown resistance mutations but fixation of CYP337B3 was detected in field populations of Helicoverpa armigera across China

Wang

QiuZan

et al. 2023

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

A simple and reliable method for discriminating between Helicoverpa armigera and Helicoverpa assulta (Lepidoptera: Noctuidae)

Cited by 5 publications

References 20 publications

A chromosome-level haplotype-resolved genome assembly of oriental tobacco budworm (Helicoverpa assulta)

A chromosome-level haplotype-resolved genome assembly of oriental tobacco budworm (Helicoverpa assulta)

A Real‐time PCR assay for rapid identification of Helicoverpa armigera (Lepidoptera: Noctuidae) from closely related species

Absence of known knockdown resistance mutations but fixation of CYP337B3 was detected in field populations of Helicoverpa armigera across China

Contact Info

Product

Resources

About