A simple and robust UPLC-SRM/MS method to quantify urinary eicosanoids

Sterz, Katharina; Scherer, Gerhard; Ecker, Josef

doi:10.1194/jlr.d023739

Cited by 67 publications

(70 citation statements)

References 52 publications

(70 reference statements)

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“…SPE involves using commercial columns pre-packed with various sorbents, which include the reversed-phase C18 (e.g., Sep-Pak, Waters Corp, Milford, MA [64, 69], polymer-based reversed phase materials (e.g., Oasis HLB, Waters Corp, Milford, MA [51, 52, 65, 70] and Strata-X, Phenomenex, Torrance, CA [54, 56]) and anion-exchange polymer-based resins [45, 53, 71, 72] cartridges. A reversed-phase C18 cartridge will allow specificity of retention based on the eicosanoids overall hydrophobicity.…”

Section: Analysis Of Oxygenated Fatty Acid Metabolitesmentioning

confidence: 99%

“…Alternative extraction procedures using ether [74], acetone/chloroform [64], hexane–isopropanol [75], and ethyl acetate/ethanol mixtures have been proposed for a wide range of tissues. LLE has proven to be a valid high-throughput alternative to SPE for the extraction of lipid metabolites from biological matrices such as urine samples [53, 76] and brain samples [64, 77]. Additionally, LLE can be used in combination with SPE to maximize the purification of oxygenated PUFA species prior to further MS analysis [66, 78–80].…”

Section: Analysis Of Oxygenated Fatty Acid Metabolitesmentioning

confidence: 99%

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Targeted lipidomic strategies for oxygenated metabolites of polyunsaturated fatty acids

Astarita

Kendall

Dennis

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

121

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Oxidation of polyunsaturated fatty acids (PUFA) through enzymatic or non-enzymatic free radical-mediated reactions can yield an array of lipid metabolites including eicosanoids, octadecanoids, docosanoids and related species. In mammals, these oxygenated PUFA mediators play prominent roles in the physiological and pathological regulation of many key biological processes in the cardiovascular, renal, reproductive and other systems including their pivotal contribution to inflammation. Mass spectrometry-based technology platforms have revolutionized our ability to analyze the complex mixture of lipid mediators found in biological samples, with increased numbers of metabolites that can be simultaneously quantified from a single sample in few analytical steps. The recent development of high-sensitivity and high-throughput analytical tools for lipid mediators affords a broader view of these oxygenated PUFA species, and facilitates research into their role in health and disease. In this review, we illustrate current analytical approaches for a high-throughput lipidomic analysis of eicosanoids and related mediators in biological samples.

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Section: Analysis Of Oxygenated Fatty Acid Metabolitesmentioning

confidence: 99%

Section: Analysis Of Oxygenated Fatty Acid Metabolitesmentioning

confidence: 99%

Targeted lipidomic strategies for oxygenated metabolites of polyunsaturated fatty acids

Astarita

Kendall

Dennis

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

121

View full text Add to dashboard Cite

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“…In support, Piper (1988) reported increased basal levels of aldosterone in E+-exposed sheep. 12-HETE has been used as a urinary biomarker of hypertension and platelet activation (Sterz et al, 2012;Yeung and Holinstat, 2011) and has vasoconstrictive properties in the kidneys, where it increases in response to angiotensin II (Ma et al, 1991;Yiu et al, 2003), which is likely increased in FT (Oliver, 1997(Oliver, , 2005.…”

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confidence: 98%

Metabolomics of fescue toxicosis in grazing beef steers

Mote

Hill

Uppal

et al. 2017

Food and Chemical Toxicology

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Fescue toxicosis (FT) results from consumption of tall fescue (Lolium arundinaceum) infected with an endophyte (Epichloë coenophiala) that produces ergot alkaloids (EA), which are considered key etiological agents of FT. Decreased weight gains, hormonal imbalance, circulating cholesterol disruption, and decreased volatile fatty acid absorption suggest toxic (E+) fescue-induced metabolic perturbations. Employing untargeted high-resolution metabolomics (HRM) to analyze E+ grazing-induced plasma and urine metabolome changes, fescue-naïve Angus steers were placed on E+ or non-toxic (Max-Q) fescue pastures and plasma and urine were sampled before, 1, 2, 14, and 28 days after pasture assignment. Plasma and urine catecholamines and urinary EA concentrations were also measured. In E+ steers, urinary EA appeared early and peaked at 14 days. 13,090 urinary and 20,908 plasma HRM features were detected; the most significant effects were observed earlier (2 days) in the urine and later (≥14 days) in the plasma. Alongside EA metabolite detection, tryptophan and lipid metabolism disruption were among the main consequences of E+ consumption. The E+ grazing-associated metabolic pathways and signatures described herein may accelerate development of novel early FT detection and treatment strategies.

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“…To evaluate LC-MS/MS method performance, we validated our method in plasma and liver samples as described previously ( 26 ). Precisions showed CVs <15% for highabundant metabolites and CVs <20% for low-abundant compounds.…”

Section: Methods Applicationmentioning

confidence: 99%

An LC-MS/MS method to quantify acylcarnitine species including isomeric and odd-numbered forms in plasma and tissues

Giesbertz

Ecker

Hann

et al. 2015

Journal of Lipid Research

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transport of activated fatty acids (acyl-CoAs) from the cytosol across the inner mitochondrial membrane into the matrix where fatty acid oxidation takes place. It has now become clear that a net effl ux of acylcarnitine species from the mitochondria into the cytosol and ultimately into plasma is particularly important in situations of impaired fatty acid oxidation to prevent accumulation of potentially toxic acyl-CoA intermediates in the mitochondrion ( 2, 3 ). How acylcarnitines are released into the extracellular space is not known, but changes in plasma and/or urinary acylcarnitine profi les are used to detect disorders in fatty acid and amino acid oxidation ( 4 ). More recently, alterations in plasma acylcarnitine profi les were described as strongly associated with obesity and type 2 diabetes ( 5-7 ). A broad spectrum of short-, medium-, and long-chain acylcarnitine species is generated from multiple metabolic routes including various fatty acid and amino acid oxidation pathways. A comprehensive quantifi cation that covers as many as possible of these entities is therefore not only important for proper clinical diagnosis of inherited enzymatic impairments, but also for basic studies of complex diseases such as the metabolic syndrome.Acylcarnitine profiles are commonly established by direct infusion ESI-MS/MS. This technology, however, does not allow the discrimination of isomeric acylcarnitine species. Furthermore, isobaric matrix interferences can lead to an overestimation of metabolite concentrations producing false positive results, in particular, for compounds with low abundance ( 8 ). Although LC-MS/ MS methods have been developed for successful separation of isomeric compounds ( 9-12 ), the identifi cation of the exact compound in the chromatogram often remains Acylcarnitines are intermediates in fatty acid and amino acid breakdown generated from the conversion of acylCoA species by the action of carnitine acyltransferases ( 1 ). The formation of carnitine conjugates is crucial for the

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A simple and robust UPLC-SRM/MS method to quantify urinary eicosanoids

Cited by 67 publications

References 52 publications

Targeted lipidomic strategies for oxygenated metabolites of polyunsaturated fatty acids

Targeted lipidomic strategies for oxygenated metabolites of polyunsaturated fatty acids

Metabolomics of fescue toxicosis in grazing beef steers

An LC-MS/MS method to quantify acylcarnitine species including isomeric and odd-numbered forms in plasma and tissues

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