A Simple and Safe Spectrophotometric Method for Iodide Determination

Sulistyarti, Hermin; Atikah, Atikah; Fardiyah, Qonitah; Febriyanti, Sita; Asdauna, Asdauna

doi:10.7454/mss.v19i2.4736

Cited by 15 publications

(11 citation statements)

References 7 publications

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“…The concentration of total protein was measured using the Bradford protein assay with bovine serum albumin as a standard [ 60 ]. The starch concentration was measured using the iodine starch method [ 61 ]. The measurements for both of these assays were performed spectrophotometrically at 595 nm and 615 nm, respectively, using an Infinite 200 PRO NanoQuant Microplate Reader (Tecan, Männedorf, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Potato Juice, a Starch Industry Waste, as a Cost-Effective Medium for the Biosynthesis of Bacterial Cellulose

Ciecholewska-Juśko

Broda

Żywicka

et al. 2021

IJMS

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In this work, we verified the possibility of valorizing a major waste product of the potato starch industry, potato tuber juice (PJ). We obtained a cost-effective, ecological-friendly microbiological medium that yielded bacterial cellulose (BC) with properties equivalent to those from conventional commercial Hestrin–Schramm medium. The BC yield from the PJ medium (>4 g/L) was comparable, despite the lack of any pre-treatment. Likewise, the macro- and microstructure, physicochemical parameters, and chemical composition showed no significant differences between PJ and control BC. Importantly, the BC obtained from PJ was not cytotoxic against fibroblast cell line L929 in vitro and did not contain any hard-to-remove impurities. The PJ-BC soaked with antiseptic exerted a similar antimicrobial effect against Staphylococcus aureus and Pseudomonas aeruginosa as to BC obtained in the conventional medium and supplemented with antiseptic. These are very important aspects from an application standpoint, particularly in biomedicine. Therefore, we conclude that using PJ for BC biosynthesis is a path toward significant valorization of an environmentally problematic waste product of the starch industry, but also toward a significant drop in BC production costs, enabling wider application of this biopolymer in biomedicine.

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Section: Methodsmentioning

confidence: 99%

Potato Juice, a Starch Industry Waste, as a Cost-Effective Medium for the Biosynthesis of Bacterial Cellulose

Ciecholewska-Juśko

Broda

Żywicka

et al. 2021

IJMS

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show abstract

“…The concentration of total protein was measured using the Bradford protein assay with bovine serum albumin as a standard (Bradford, 1976). The starch concentration was measured using the iodine starch method (Sulistyarti et al, 2015). The measurements for both of these assays were performed spectrophotometrically at 595 nm and 615 nm respectively, using an Infinite 200 PRO NanoQuant Microplate Reader (Tecan, Switzerland).…”

Section: Determination Of Ph Protein and Carbohydrate Concentration In Pj Mediummentioning

confidence: 99%

Potato juice, a starch industry waste, as a cost-effective medium for the biosynthesis of bacterial cellulose

Ciecholewska-Juśko

Broda

Żywicka

et al. 2021

Preprint

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The unique properties of bacterial cellulose (BC) make it of great interest for numerous branches of industry. Nevertheless, the high cost of the dedicated, microbiological medium used for BC production significantly hinders possibility of widespread use. Searching for an alternative, we turned our attention to potato tuber juice (PJ), a major waste product of the potato starch industry. We verified the possibility of using PJ as a cost-effective, ecological-friendly medium that yielded BC with properties equivalent to those from conventional commercial Hestrin-Schramm medium. The BC yield from PJ medium (>4 g/L) was comparable, despite the lack of any pre-treatment. Likewise, the macro- and microstructure, physicochemical parameters, and chemical composition showed no significant differences between PJ and control BC. Importantly, BC obtained from PJ was not cytotoxic against fibroblast cell line L929 in vitro and did not contain any hard-to-remove impurities. These are very important aspects from an application standpoint, particularly in biomedicine. Therefore, we conclude that using PJ for BC biosynthesis is a path towards significant valorization of an environmentally problematic waste product of the starch industry and can help ultimately lower BC production costs.

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“…Muralidhara (1998) (Muralidhara, 1998). Sulistyarti (2015) melaporkan bahwa iodida teroksidasi oleh iodat membentuk iodium yang dengan adanya amilum membentuk larutan kompleks biru jernih yang dapat dideteksi menggunakan spektrofotometer pada λ 618 nm. Oleh karena itu, penelitian ini bertujuan untuk mengembangkan metode analisis merkuri(II) secara spektrofotometri secara tidak langsung (indirect spectrophotometry) berdasarkan pembentukan kompleks biru amilum-iodium menggunakan system pereaksi iodida-iodat.…”

Section: Pendahuluanunclassified

“…Hanya ion iodida yang tersisa akan dioksidasi oleh iodat menjadi iodium yang menghasilkan warna biru dengan absorbansi yang lebih rendah (Sulistyarti et al, 2015).…”

Section: Pendahuluanunclassified

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Metode Spektrofotometri Secara Tidak Langsung untuk Penentuan Merkuri(II) berdasarkan Pembentukan Kompleks Biru Iodium-Amilum

et al. 2019

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Metode spektrofotometri secara tidak langsung telah dikembangkan untuk analisis merkuri(II) berdasarkan perbedaan absorbansi kompleks biru amilum-iodium antara larutan sampel yang mengandung ion merkuri(II) dan larutan blanko yang tidak mengandung ion merkuri(II) dalam sistem pereaksi yang berisi iodida, iodat dan amilum. Pada larutan blanko, semua ion iodida (I-) dalam sistem pereaksi dioksidasi oleh iodat menjadi iodium yang dengan adanya amilum membentuk kompleks biru iodium-amilum dan terdeteksi secara spektrofotometri pada 618 nm. Namun, bila sampel mengandung ion merkuri(II), maka sebagian ion iodida akan terikat oleh ion merkuri(II) dan membentuk kompleks tetraiodomerkurat(II), sehingga hanya sisa iodida yang dioksidasi oleh ion iodat menjadi iodium dan membentuk warna biru dengan absorbansi yang lebih rendah dibandingkan absorbansi larutan blanko. Hasil penelitian menunjukkan bahwa perbedaan absorbansi sebanding dengan konsentrasi iodida yang terikat pada ion merkuri(II) sehingga sebanding pula dengan konsentrasi merkuri(II) dalam sampel. Metode yang dikembangkan dioptimasi terhadap beberapa parameter kimia, antara lain konsentrasi larutan iodida, iodat, amilum, dan pH larutan dan pada kondisi optimum memberikan kisaran linier 1 – 9 mg L-1 ion merkuri(II) dengan R2 0,9983, dengan LOD 0,44 mgL-1. Metode ini cukup selektif terhadap ion kobalt(II) dan timbal(II), namun ion tembaga(II) dan perak(I) mengganggu pengukuran. Metode ini telah divalidasi menggunakan metode adisi standar yang diaplikasikan ke dalam sampel limbah pertambangan emas dari Lombok dengan hasil yang memuaskan.Indirect Spectrophotometry for Mercury(II) Determination Based on The Formation of Blue Starch-Iodine Complex. Indirect spectrophotometric method for determining mercury(II) concentration has been successfully developed based on the difference of the absorbance of the blue starch-iodine complex in the absence and in the presence of mercury(II). In the absence of mercury(II), all iodide ions (I-) in the reagent system are oxidized by iodate to iodine, which in the presence of starch formed a clear blue complex of starch-iodine detected spectrophotometrically at 618 nm. However, if mercury presents in the sample, some of the iodide ions are bound to mercury(II) ion forming tetraiodomercurate(II) complex, and thus, only the remaining of iodide is oxidized by iodate to iodine resulting in lower absorbance of the blue color. The results showed that the delta absorbance was proportional to the concentration of iodide bound to mercury and thus proportional to mercury(II) concentration. To achieve the sensitivity, the method was optimized to the main chemical parameters, such as the concentration of iodide, iodine, iodate, starch, and pH solution. Selectivity of the method was also studied by investigating the effect of interfering ions of copper(II), cobalt (II), lead(II), and silver (I). Under these optimum conditions, the method showed linearity measurements from 1 – 9 mg L-1 mercury(II) with correlation (R2) of 0.996. The method was also successfully applied to determine mercury(II) from small-scale gold mining tailing waste from West Lombok, Indonesia and is prospective for analysis of mercury(II) in mining waste.

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A Simple and Safe Spectrophotometric Method for Iodide Determination

Cited by 15 publications

References 7 publications

Potato Juice, a Starch Industry Waste, as a Cost-Effective Medium for the Biosynthesis of Bacterial Cellulose

Potato Juice, a Starch Industry Waste, as a Cost-Effective Medium for the Biosynthesis of Bacterial Cellulose

Potato juice, a starch industry waste, as a cost-effective medium for the biosynthesis of bacterial cellulose

Metode Spektrofotometri Secara Tidak Langsung untuk Penentuan Merkuri(II) berdasarkan Pembentukan Kompleks Biru Iodium-Amilum

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