A Simple and Sensitive Method for the Voltammetric Analysis of Theobromine in Food Samples Using Nanobiocomposite Sensor

Peng, Yingqiong; Zhang, Wenjuan; Chang, Juan; Huang, Yaoping; Chen, Li; Deng, Hong Wen; Huang, Zhong; Wen, Yangping

doi:10.1007/s12161-017-0867-5

Cited by 19 publications

(5 citation statements)

References 27 publications

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“…A variety of analytical techniques and sample preparation procedures have been developed and registered for determination and separation of these three methylxanthines in plants, environmental samples, drugs, food, and biological fluids. These proposed methods include UV-Vis spectrophotometry [22–24], thin-layer chromatography (TLC) [25], ion chromatography (IC) [26], FT-Raman spectrometry [27], IR spectrometry [28], capillary electrophoresis (CE) [29, 30], NMR spectroscopy [31], mass spectroscopy [32], gas chromatography-mass spectrometry (GC-MS) [15], high-performance thin-layer chromatography (HPTLC) [33, 34], voltammetry [35, 36], and high-performance liquid chromatography (HPLC) which was reported as the most frequently used approach [1, 2, 10, 37–49].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Determination of Methylxanthines in Commercial Brands of Tea Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry

Aqel

Al-Mulla

Al-Rifai

et al. 2019

International Journal of Analytical Chemistry

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Recently, chromatographic techniques have the potential to be greener in order to reduce the environmental impact. In this work, a new simple, sensitive, efficient, and green analytical method based on UHPLC-MS has been developed for a quick determination of methylxanthines including caffeine, theobromine, and theophylline in tea. Under the optimum conditions, a baseline separation has been achieved within 30 seconds, using isocratic elution consisting of 90% water and only 10% acetonitrile at 0.5 mL/min flow rate (3 mL acetonitrile per hour). The mass spectrometer was operated with the SIR mode in ESI+. The developed method was found to be linear in the range of 0.03–5 μg/mL, with correlation coefficients greater than 0.9995 for the three compounds. The respective values of LOD were found to be 0.025, 0.015, and 0.01 μg/mL for caffeine, theobromine, and theophylline, respectively. The proposed assay was applied to 30 commercial tea samples of different brands. Both caffeine and theobromine were found in all tea samples with maximum concentration in sample no. 15, corresponding to 32.6 and 2.72 mg/g of caffeine and theobromine, respectively. On the contrary, theophylline was not detected at all in most samples. When compared with all previous studies that dealt with the same compounds in different matrices, the developed method was found to be the fastest, allowing high-throughput analyses with more than 100 samples/h. The results prove that the method is suitable for routine analysis of methylxanthines and to distinguish the quality of tea samples of various brands.

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Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Determination of Methylxanthines in Commercial Brands of Tea Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry

Aqel

Al-Mulla

Al-Rifai

et al. 2019

International Journal of Analytical Chemistry

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“…The enhancement of conductivity for the electrode can be attributed to that the bundles become more individual by the modification of CMC, therefore, the individual electrochemical property gets fully utilization. 23 The electrode effective area was investigated by chronocoulometry (Fig. 2B), and calculated by the plot of Qt 1/2 as the following equation: surface area, also supporting that the original bundles of MWCNTs are dispersed uniformly by CMC.…”

Section: Resultsmentioning

confidence: 55%

“…22 More interesting, wrapping of CMC to CNTs can enhances the adsorption capacity of CNTs toward target molecule because of the abundant hydroxyl and carboxylic groups in CMC. 23 In our pervious study, a nanocomposite was successfully fabricated with the aid of CMC, and the composite showed enhanced electrode stability in water, good binding capacity, and high electrochemical activities toward Uric acid (UA) guanine (G) and adenine (A). 24 Inspired by the aforementioned reports, a nanohybrid sensor was facilely fabricated based on the CMC functionalized MWCNTs in the present work.…”

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confidence: 99%

Highly Sensitive Detection of Carbendazim and Its Electrochemical Oxidation Mechanism at a Nanohybrid Sensor

Liao

Huang

et al. 2019

J. Electrochem. Soc.

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Sensitive and rapid detection of pesticides in food has become an important and crucial research area due to the extensive use of agriculture residues and stringent environmental protection acts. In present work, an electrochemical nanohybrid sensor was facilely developed based on carboxymethyl cellulose (CMC) fuctionalized carbon nanotubes (CNTs). The sensor shows high sensitivity, excellent stability and good anti-interference capability in the detection of carbendazim (MBC) by differential pulse voltammetry method. Under optimized conditions, it exhibits a wide linearity of 0.03 μmol•L −1 -10 μmol•L −1 with a low detection limit of 0.015 μmol•L −1 . In real samples analyses for peer and kiwifruit, the proposed sensor shows good recoveries which are comparable with that of the conventional HPLC method. In addition, the oxidation mechanism of MBC at the nanohybrid sensor was studied using voltammetric methods and validated according to the density functional theory (DFT) calculation. The results reveal that the oxidation of carbendazim is a typical adsorption-controlled electrochemical oxidation process proceeding with four protons and four electrons. The proposed nanohybrid sensor is a promising alternative to the currently used methods for carbendazim detection and our results provide a new insight in MBC electrochemical oxidation mechanism.

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“…8 Development of a high-performance MeX sensor with multiplexing ability is thus crucial for effective diagnosis of caffeine intoxication (a.k.a caffeinism) 2 and other diseases, therapeutic drug monitoring, quality control of consumer products in the food and pharmaceutical industries, as well as for forensics and veterinary science. Conventional methods for MeX detection are based on high-performance liquid chromatography, near infrared spectroscopy, immunoassay, voltammetry and fluorescence, 6,[9][10][11][12][13][14][15][16][17] but not all of these methods allow multiplexed detection of MeX in real-time with minimal sample preparation and high performance.…”

Section: Introductionmentioning

confidence: 99%

SERS multiplexing of methylxanthine drug isomers via host–guest size matching and machine learning

et al. 2021

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A Simple and Sensitive Method for the Voltammetric Analysis of Theobromine in Food Samples Using Nanobiocomposite Sensor

Cited by 19 publications

References 27 publications

Rapid and Sensitive Determination of Methylxanthines in Commercial Brands of Tea Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry

Rapid and Sensitive Determination of Methylxanthines in Commercial Brands of Tea Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry

Highly Sensitive Detection of Carbendazim and Its Electrochemical Oxidation Mechanism at a Nanohybrid Sensor

SERS multiplexing of methylxanthine drug isomers via host–guest size matching and machine learning

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