A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes

Safina, Gulnara; Ludwig, Roland; Gorton, Lo

doi:10.1016/j.electacta.2009.10.052

Cited by 70 publications

(45 citation statements)

References 30 publications

(34 reference statements)

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“…Optimization studies were also performed with a CtCDH modified electrode Operational stability studies at an applied potential of 0.5 V were performed for 72 hours with GOx and CtCDH modified electrodes (using Os(bpy) 2 PVI) in glucose saturated buffer (Figure 4). Both electrodes displayed immediate decreases in response, in contrast to previous reports where CtCDH modified anodes maintained their stability for up to 11 hours [38,41]. A half-life of ca.…”

Section: Resultscontrasting

confidence: 44%

Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes

et al. 2012

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Abstract:Nanoporous and planar gold electrodes were utilised as supports for the redox enzymes Aspergillus niger glucose oxidase (GOx) and Corynascus thermophilus cellobiose dehydrogenase (CtCDH). Electrodes modified with hydrogels containing enzyme, Os-redox polymers and the cross-linking agent poly(ethylene glycol)diglycidyl ether (PEGDGE) were used as biosensors for the determination of glucose and lactose. Limits of detection of 6.0 (± 0.4), 16.0 (± 0.1) and 2.0 (± 0.1) µM were obtained for CtCDH modified lactose and glucose biosensors and GOx modified glucose biosensors, respectively, at nanoporous gold electrodes. Biofuel cells comprised of GOx and CtCDH modified gold electrodes were utilised as anodes, together with Myrothecium verrucaria bilirubin oxidase (MvBOD) or Melanocarpus albomyces laccase (rMaLc) as cathodes, in biofuel cells. A maximum power density of 41 µW/cm 2 was obtained for a CtCDH/MvBOD biofuel cell in 5 mM lactose and O 2 saturated buffer (pH 7.4, 0.1 M phosphate, 150 mM NaCl).2

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Section: Resultscontrasting

confidence: 44%

Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes

et al. 2012

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“…Enzymes were immobilized on screen-printed carbon electrodes. The biosensors were able to detect lactose in a concentration range between 0.5-200 μM and 0.5-100 μM, respectively, with a limit of detection of 250 nM (Safina et al 2010).…”

Section: Lactosementioning

confidence: 99%

Application of Enzyme Biosensors in Analysis of Food and Beverages

et al. 2011

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The importance of analyses of different parameters in food products and monitoring of a production process requires quick and reliable analytical methods and devices. For this purpose, biosensors can be a suitable option, whereas most of the current quality control techniques are time consuming, expensive, and unpractical. In this paper, we describe biosensors developed for analysis of different components present in food samples, namely, glucose, fructose, sucrose, lactose, lactic, malic, acetic, ascorbic, citric and amino acids, ethanol, glycerol, and triglyceride. Biosensors showed desirable sensitivity, selectivity, and response time required for various applications. They are often designed to avoid interference from components present in a complex sample to be analyzed.

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“…Como consequência, têm mostrado alta versatilidade de aplicações, tanto na forma de eletrodo convencional, quanto na forma de eletrodo modificado. [21][22][23][24][25][26][27][28][29][30][31][32][33] Ademais, dependendo da aplicação, esses eletrodos podem ser empregados muitas vezes, barateando ainda mais o custo das determinações analíticas.…”

Section: Os Eletrodos De Mercúrio (Dme -Dropping Mercury Electrode Hunclassified

Construção e aplicação de um minissensor de filme de bismuto utilizando materiais de baixo custo para determinações voltamétricas in loco

Figueiredo-Filho¹,

Janegitz²,

Faria³

et al. 2012

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A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes

Cited by 70 publications

References 30 publications

Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes

Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes

Application of Enzyme Biosensors in Analysis of Food and Beverages

Construção e aplicação de um minissensor de filme de bismuto utilizando materiais de baixo custo para determinações voltamétricas in loco

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