A Simple Comparative Method of measuring Red Cell Viability

Doebbler, G. F.; Dwyer, R. F.; Rinfret, A. P.

doi:10.1038/195912a0

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1964

1978

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Cited by 28 publications

(12 citation statements)

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“…Addition to the remaining 250 ml red cells of 250 ml NaCl 0.9% solution containing 87.5 g glycerol and 10 g sorbitol. 4. Filling of the stainless steel container with 500 ml of the glycerolized red cell suspension.…”

Section: Resultsmentioning

confidence: 99%

“…Cr51 double tag method [4]. Approximately 10 ml of a suspension of fresh, autologous red cells, labelled with 5 fiC of Cr51, were injected, and blood was collected after 10, 20, and 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…Then the viability in vivo of frozen, thawed and reconstituted red cells was investigated by the Cr51 double tag procedure [4].…”

mentioning

confidence: 99%

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Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen

Krijnen¹,

Wit²,

Kuivenhoven³

et al. 1964

Vox Sanguinis

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen

Krijnen¹,

Wit²,

Kuivenhoven³

et al. 1964

Vox Sanguinis

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“…The measurement of the survival in vivo of preserved red cells has been performed by means of the double 51chromium technique of Doebbler et al [2]. In this technique the recipient and donor red cells are each labelled with different amounts of 51chromium.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Double ^51CR Technique

Szymanski¹,

Valeri²,

Hickey³

1968

Vox Sanguinis

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The post-transfusion survival of multiple units of concentrated red cells was measured by ^51chromium automated differential agglutination and double 51chromium technics. Since the mean 24 h survivals were similar with both assay methods, these methods are considered acceptable in evaluating red cell preservation. Analysis of the paired data, however, revealed a significant difference in the results, indicating that an average of 3 % of ^51chromium eluted from red cells during the first 24 h.

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“…Survival times were determined by tagging a sample of blood with chromium-51 and following the rate of isotope decay according to the method of Gray and Sterling [3] and of Doebbler et al [4], Approximately 20 ml of blood were collected in ACD anticoagulant and labeled with 100-200 /tCi of Na2 51Cr 04 (sp. act.…”

mentioning

confidence: 99%