A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter

“…1 filter paper were fixed in the center of each flask to absorb metabolic ~4CO2. Glass cups containing absorbed 1"CO 2 were transferred to vials containing 15 ml dioxane based scintillation cocktail (12). Radioactivity was determined in a Beckman LS 7000 Liquid scintillation counter.…”

Section: Fatty Acid Oxidation In Vitromentioning

confidence: 99%

Altered maternal thyroid function: Effect of L-carnitine supplementation on fetal and neonatal myocardial free fatty acid oxidation,in vitro

Kumar

1998

Indian J Clin Biochem

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Effect of L-camitine supplementation on myocardial free fatty acid oxidation, in vitro, in offsprings born of hypothyroid and hyperthyroid mothers was studied in rats. L-camitine supplementation stimulated myocardial fatty acid oxidation during gestational period in offspring born of control and hyperthyroid mothers. In contrast L-camitine supplementation induced stimulation in myocardial fatty acid oxidation was very less in fetuses born of hypothyroid mothers. However, in neonates bom of hypothyroid mothers L-carnitine stimulated myocardial fatty acid oxidation to a great extent. The results suggested that during maternal hypothyroidism low availability of thyroid hormones to fetuses through maternal circulation and availability of less camitine to neonates due to hypolactation might be responsible for decreased myocardial free fatty acid utilization. In neonates born of hypothyroid mothers and with cardiac energy insufficiency parenteral carnitine supplementation might be of great help to prevent mortality and morbidity of such offsprings.

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“…The radioactivity of 2 ml of the combined fractions mixed with 12 ml of Bray's solution [13] was determined in a Mark II liquid scintillation counter and corrected for quenching by internal standard. 98.4-102.3% of the radioactivity placed on the column was recovered in the combined eluates.…”

Section: Determination Of Pyruvate Decarboxylation By the Perfused Hementioning

confidence: 99%

The effect of insulin on pyruvate dehydrogenase interconversion in heart muscle of alloxan-diabetic rats

Öhlén¹,

Siess²,

Löffler³

et al. 1978

Diabetologia

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Summary.Evidence is presented for regulation by insulin of pyruvate dehydrogenase (PDH) interconversion in rat heart muscle in vivo and in vitro. In the alloxan diabetic rat the active (dephospho) enzyme amounted only to 12% of total PDH and was restored to 42% by insulin. Antilipolytic treatment of the diabetic animals was ineffective, indicating that the action of insulin was independent of a lowering of plasma non-esterified fatty acid concentration. On perfusion of isolated hearts from diabetic rats in the presence of glucose the proportion of pyruvate dehydrogenase in the active form remained low but was fully restored upon addition of insulin (2 mU/ml) to the medium. No effect of insulin was obtained in the absence of glucose. The correlation between the rate of pyruvate decarboxylation in the perfused heart and of pyruvate dehydrogenase activity, in vitro, suggests that in the diabetic heart the entry of pyruvate into the citric acid cycle is largely controlled by covalent modification of the pyruvate dehydrogenase complex rather than by feedback inhibition. The possible role of insulin therein is discussed.

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A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter

Cited by 7,665 publications

References 9 publications

Development of phospholipid synthesizing enzymes in castor bean endosperm

Development of phospholipid synthesizing enzymes in castor bean endosperm

Altered maternal thyroid function: Effect of L-carnitine supplementation on fetal and neonatal myocardial free fatty acid oxidation,in vitro

The effect of insulin on pyruvate dehydrogenase interconversion in heart muscle of alloxan-diabetic rats

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