A simple electrochemical approach to fabricate functionalized MWCNT-nanogold decorated PEDOT nanohybrid for simultaneous quantification of uric acid, xanthine and hypoxanthine

Sen, Sarani; Sarkar, Priyabrata

doi:10.1016/j.aca.2020.03.060

Cited by 44 publications

(16 citation statements)

References 51 publications

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“…A sharp increase in the reduction peak current was observed for each addition after dipping the electrode into a particular solution for 240 s at an applied potential of −0.1 V (i.e., deposition potential). The peak was shifted to a negative potential as the concentration of PT enhanced, indicating a diffusion-controlled process [ 40 ]. The concentration-dependent linear plot depicts good linearity ( Figure 9B , Figure 9C ) with a calibration equation of I p (μA) = 3.5735 [PT] + 12.018 ( R ² = 0.9871) for the range of 0.1–11 μM, I p (μA) = 0.2916 [PT] + 3.7526 ( R ² = 0.9936) for 3–15 nM, I p (μA) = 19.176 [PT] + 4.2723 ( R ² = 0.9367) for 0.03–0.15 nM.…”

Section: Resultsmentioning

confidence: 99%

A nonenzymatic reduced graphene oxide-based nanosensor for parathion

Sen¹,

Roy²,

Sanyal³

et al. 2022

Beilstein J. Nanotechnol.

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Organophosphate-based pesticides (e.g., parathion (PT)) have toxic effects on human health through their residues. Therefore, cost-effective and rapid detection strategies need to be developed to ensure the consuming food is free of any organophosphate-residue. This work proposed the fabrication of a robust, nonenzymatic electrochemical-sensing electrode modified with electrochemically reduced graphene oxide (ERGO) to detect PT residues in environmental samples (e.g., soil, water) as well as in vegetables and cereals. The ERGO sensor shows a significantly affected electrocatalytic reduction peak at −0.58 V (vs Ag/AgCl) for rapid quantification of PT due to the amplified electroactive surface area of the modified electrode. At optimized experimental conditions, square-wave voltammetric analysis exhibits higher sensitivity (50.5 μA·μM−1·cm−2), excellent selectivity, excellent stability (≈180 days), good reproducibility, and repeatability for interference-free detection of PT residues in actual samples. This electrochemical nanosensor is suitable for point-of-care detection of PT in a wide dynamic range of 3 × 10−11–11 × 10−6 M with a lower detection limit of 10.9 pM. The performance of the nanosensor was validated by adding PT to natural samples and comparing the data via absorption spectroscopy. PT detection results encourage the design of easy-to-use nanosensor-based analytical tools for rapidly monitoring other environmental samples.

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Section: Resultsmentioning

confidence: 99%

A nonenzymatic reduced graphene oxide-based nanosensor for parathion

Sen¹,

Roy²,

Sanyal³

et al. 2022

Beilstein J. Nanotechnol.

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“…Scan-rate effect.-To study the scan-rate effect on the electrochemical behavior of Xn and UA on BP/PET platform, CV was performed by applying various potential scan-rates. Figures 3A Human urine polymerized film of 6-thioguanine (6-TG) modified GCE 22 1-500 2-1600 0.30 0.06 Human urine and serum Reduced graphene oxide modified GCE 23 10-60 20-120 0.11 0.088 Human blood plasma and urine cathodically pretreated poly(1-aminoanthraquinone) (PAAQ)-modified electrode 24 -35-420 -11.5 Human urine Au-PEDOT-fMWCNT/GCE 25 0.05-175 0.1-800 0.091 0.199 Fish extracts, Human urine and serum Pre-anodized nontronite-coated screen-printed carbon electrodes (NSPEs * ) 26 2-40 2-40 0.07 0.42 blood plasma and urine samples Toray paper 20 7-300 100-1000 6. and 3B shows that the anodic peak current increase with increase in scan-rate. Figure 3 shows that anodic current is directly proportional to the scan-rate υ½, as per Randle-Sevick equation.…”

Section: Resultsmentioning

confidence: 99%

Miniaturized Disposable Buckypaper-Polymer Substrate Based Electrochemical Purine Sensing Platform

Salve

Amreen

Rajurkar

et al. 2020

ECS J. Solid State Sci. Technol.

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Herein, a novel, low-cost and disposable electrochemical sensor is presented by using recycled base material polyethyleneterephthalate (PET) obtained from a disposable drinking water bottle as a sensing platform. Bucky paper (BP) was used as a working electrode material. The CO2 laser was used for fabricating sensing substrate and the desired three electrode pattern of BP. This was attached on the PET substrate using a double sided tape. The lamination process was used to define the geometric area and for insulating the electrode. The determination of Xanthine (Xn) and Uric Acid (UA) were carried out as a proof of concept with unmodified BP surface. The electrochemical behavior of Xn and UA was studied using a cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometery (CA). The calibration curve for Xn and UA were obtained in the linear range 10 μM−500 μM and 50 μM−1000 μM respectively using and CA. The LOD obtained for Xn and UA was 6 μM and 22.5 μM respectively. Finally, the proposed method was applied for determination of Xn and UA in human serum sample with good selectivity and high sensitivity.

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“…Before modification of GCE, it was cleaned with alumina powder (0.05 μm), followed by 5 min of ultrasonication in deionized water and ethanol (1:1). 5,6 Lysozyme@Au-GSH (2 μL) was drop cast with 1 μL of Nafion on the GCE and dried at room temperature for 20 min. The impedance spectra were recorded using an IVIUMStat…”

Section: Fabrication Of Electrode For Biosensingmentioning

confidence: 99%

“…suitable for the fabrication of nanosensors to enhance the sensitivity, specificity, and detection limits of target analytes. 5,6 Nanoparticles can be synthesized through physical, chemical, or biological routes. [7][8][9] Biological processes are more economic, safe, and environmentfriendly as there is minimum use of chemical compounds and hence less chance of producing toxic substances.…”

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confidence: 99%

Impedance nanobiosensor based on enzyme‐conjugated biosynthesized gold nanoparticles for the detection of Gram‐positive bacteria

Sen,

Sarkar

2023

Biotechnology Progress

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In this report, gold nanoparticles (GNPS) were synthesized using cell‐free extracts of seven different isolates, namely, Pseudomonas aerogenosa CEBP2, Pseudomonas sp. CEBP1, Pseudomonas pseudoalcaligenes CEB1G, Acinetobactor baumani CEBS1, Cuprividus sp. CEB3, Micrococcus luteus CUB12, and Pandoraea sp. CUB2S. The spectroscopic (UV–vis, FTIR, DLS, XRD, EDS) and microscopic (FESEM, TEM) results confirm the reduction of Au3+ to Au0 in the presence of biomolecules having reducing as well as self‐stabilizing activity. In this green synthesis approach, the average particle size of biosynthesized GNPS might vary (4–60 nm) depending on the bacterial species, pH of the media, incubation time, and temperature. In this study, GSH‐modified BSGNPs (Au‐GSH) have shown antimicrobial activity with better stability against Gram‐positive bacteria. After conjugation of lysozyme with Au‐GSH (lyso@Au‐GSH), the zone of inhibition was enhanced from 12 to 23 mm (Au‐GSH). The TEM study shows the spherical GNP (16.65 ± 2.84) turns into a flower‐shaped GNP (22.22 ± 3.12) after conjugation with lysozyme due to the formation of the protein corona. Furthermore, the nanobioconjugate (lyso@Au‐GSH) was immobilized with Nafion on a glassy carbon electrode to fabricate a label‐free impedance biosensor that is highly sensitive to monitor changes in the transducer surface due to biomolecular interactions. The uniquely designed biosensor could selectively detect Gram‐positive bacteria in the linear range of 3.0 × 101–3 × 1010 cfu mL−1 with RE <5%. The proposed simplest biosensor exhibited good reproducibility (RSD = 3.1%) and excellent correlation (R2 = 0.999) with the standard plate count method, making it suitable for monitoring Gram‐positive bacterial contamination in biofluids, food, and environmental samples.

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A simple electrochemical approach to fabricate functionalized MWCNT-nanogold decorated PEDOT nanohybrid for simultaneous quantification of uric acid, xanthine and hypoxanthine

Cited by 44 publications

References 51 publications

A nonenzymatic reduced graphene oxide-based nanosensor for parathion

A nonenzymatic reduced graphene oxide-based nanosensor for parathion

Miniaturized Disposable Buckypaper-Polymer Substrate Based Electrochemical Purine Sensing Platform

Impedance nanobiosensor based on enzyme‐conjugated biosynthesized gold nanoparticles for the detection of Gram‐positive bacteria

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