A simple ELISA procedure for HIV-1 based on the enzyme β-lactamase

Horse radish peroxidase, alkaline phaosphatase and beta-D-galactosidase are widely used as labels in the development of enzyme immunoassays (EIAs). Enzyme beta-lactamase, though introduced as a label in late seventies has not yet become very popular inspite of having the necessary features of an enzyme to be used in EIAs. The present article reviews assays developed with this enzyme, highlights its salient features and brings out an argument in favour of its wide spread use in EIAs.

show abstract

A Simple and Inexpensive Glucose Oxidase Substrate System for Enzyme Immunoassay

Blais

Yamazaki

1992

Immunological Investigations

View full text Add to dashboard Cite

A simple and inexpensive glucose oxidase substrate system was developed for enzyme immunoassay. This system is based on the formation of a coloured complex between polyvinyl alcohol or starch and iodine produced from iodide in the presence of hydrogen peroxide generated by the glucose oxidase reaction. The rate of iodine production was enhanced by the supplementation of molybdate. In an enzyme immunoassay for anti-Salmonella antibodies using glucose oxidase as the indicator enzyme, the molybdate-enhanced polyvinyl alcohol- and starch-glucose-iodide substrate systems were as sensitive as a conventional glucose oxidase assay system employing horseradish peroxidase as a secondary enzyme and a suitable hydrogen-donating chromogen.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A simple ELISA procedure for HIV-1 based on the enzyme β-lactamase

Cited by 3 publications

References 11 publications

Chromogenic redox assay for β-lactamases yielding water-insoluble products

Chromogenic redox assay for β-lactamases yielding water-insoluble products

A Comparison of Performances of Four Enzymes Used in Elisa with Special Reference to β-Lactamase

A Simple and Inexpensive Glucose Oxidase Substrate System for Enzyme Immunoassay

Contact Info

Product

Resources

About