A simple experiment to show photodynamic inactivation of bacteria on surfaces

Caminos, Daniel A.; Durantini, Edgardo N.

doi:10.1002/bmb.11

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…In 2007, N. Durantini et al developed new suitable approaches to visualize the PDI of bacteria immobilized on agar surfaces. Cationic TTAP 4+ and anionic TPPS 4 4− were analyzed as model on E. coli . Later, they evaluated the photodynamic activity of A 3 B 3+ porphyrin in vitro to inactivate colonies of E. coli cells immobilized on agar surfaces using these new approaches.…”

Section: Types Of Porphyrin Based Compounds For Anti‐microbial Pdi Trmentioning

confidence: 99%

A Perspective on the Trends and Challenges Facing Porphyrin‐Based Anti‐Microbial Materials

Gan

Gao

et al. 2016

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106

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The emergence of multidrug resistant bacterium threatens to unravel global healthcare systems, built up over centuries of medical research and development. Current antibiotics have little resistance against this onslaught as bacterium strains can quickly evolve effective defense mechanisms. Fortunately, alternative therapies exist and, at the forefront of research lays the photodynamic inhibition approach mediated by porphyrin based photosensitizers. This review will focus on the development of various porphyrins compounds and their incorporation as small molecules, into polymers, fibers and thin films as practical therapeutic agents, utilizing photodynamic therapy to inhibit a wide spectrum of bacterium. The use of photodynamic therapy of these porphyrin molecules are discussed and evaluated according to their electronic and bulk material effect on different bacterium strains. This review also provides an insight into the general direction and challenges facing porphyrins and derivatives as full-fledged therapeutic agents and what needs to be further done in order to be bestowed their rightful and equal status in modern medicine, similar to the very first antibiotic; penicillin itself. It is hoped that, with this perspective, new paradigms and strategies in the application of porphyrins and derivatives will progressively flourish and lead to advances against disease.

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Section: Types Of Porphyrin Based Compounds For Anti‐microbial Pdi Trmentioning

confidence: 99%

A Perspective on the Trends and Challenges Facing Porphyrin‐Based Anti‐Microbial Materials

Gan

Gao

et al. 2016

Small

125

106

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“…The light was filtered through a 2.5 cm glass cuvette filled with water to absorb heat. Irradiation of the cultures was performed as previously described [17]. Experiments were carried out at room temperature and there was no heating during irradiation.…”

Section: Generalmentioning

confidence: 99%

Photodynamic properties and photoinactivation of Candida albicans mediated by brominated derivatives of triarylmethane and phenothiazinium dyes

Alvarez

Milanesio

et al. 2014

Photodiagnosis and Photodynamic Therapy

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“…In particular, this cationic porphyrin proved to be an effective PS to kill microorganisms in similar experiments, which simulate a susceptibility test. 42,45,63 With these results, CS−PPIX can be considered a promising photodynamic polymer to treat contaminated food and packaging surfaces.…”

Section: Formation Of Omentioning

confidence: 97%

Development of New Photoinactivation Strategies for Microbial Decontamination in Fruits and Packaging

Larrea,

Gsponer,

Durantini

et al. 2023

ACS Food Sci. Technol.

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The consumption of fruits and vegetables in the daily diet is an adequate condition for obtaining a healthy life. Therefore, decontamination is crucial to reducing the appearance of potentially dangerous microorganisms in these foods. In this work, a conjugate (CS−PPIX) of natural origin was synthesized from the covalent union of protoporphyrin IX (PPIX) and chitosan (CS) by amide bonds. This photosensitizer was developed to reduce the microbial load in fruits and food packaging through the photodynamic inactivation of microorganisms (PDI). A film of CS−PPIX showed an appreciable contact surface. The conjugation of PPIX to CS was confirmed by IR spectra. The absorption and emission spectra of the conjugate exhibited the characteristic PPIX bands, accompanied by a fluorescence quantum yield of 0.10. Photodynamic investigation indicated that CS−PPIX produced singlet molecular oxygen (Φ Δ = 0.40) in solution. Also, this compound was able to generate superoxide anion radicals with the assistance of the reductant nicotinamide adenine dinucleotide and efficiently photo-oxidize the amino acid L-tryptophan. PDI in vitro experiments were performed with the Gram-positive bacterium Staphylococcus aureus. Bacteria were eliminated (>99.9999% reduction in survival) when cell suspensions were treated with 1 μM CS−PPIX upon 30 min of irradiation with white light. Furthermore, no microbial growth was detected in cells immobilized on agar surfaces in the CS−PPIX-treated areas. Therefore, the PDI approach was evaluated to reduce the contamination of S. aureus on fruit surfaces and food containers. After treating the surfaces of apple and pear pieces with 10 nmol of CS−PPIX, a greater than 99.9% reduction in bacterial survival was observed upon an irradiation of 30 min. This conjugate was also effective in the photodynamic decontamination of polyethylene bags and polyethylene terephthalate packaging materials. Therefore, the CS−PPIX conjugate presents adequate properties to reduce the microbial load and preserve fresh foods.

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A simple experiment to show photodynamic inactivation of bacteria on surfaces

Cited by 10 publications

References 24 publications

A Perspective on the Trends and Challenges Facing Porphyrin‐Based Anti‐Microbial Materials

A Perspective on the Trends and Challenges Facing Porphyrin‐Based Anti‐Microbial Materials

Photodynamic properties and photoinactivation of Candida albicans mediated by brominated derivatives of triarylmethane and phenothiazinium dyes

Development of New Photoinactivation Strategies for Microbial Decontamination in Fruits and Packaging

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