A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

Abstract

A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result.

Fluorescence detection of three types of pollutants based on fluorescence resonance energy transfer and its comparison with colorimetric detection

“…6,7 However, the overuse of cyromazine has been proven to cause problems with food contamination of animal origin. 8 Several extended toxicological tests have proved that it would cause mammary tumours in mice, and its structure is similar to other mammary tumour-producing analogues, thus posing a potential threat to the environment and human health 9,10 However, cyromazine can be easily metabolized in both plants and animals to form melamine, 11 and its excessive accumulation results in kidney failure because of the formation of insoluble melamine-cyanurate acid crystals. 12 Therefore, cyromazine has been classied as a potential carcinogenic drug by the Environmental Protection Agency of the US, 13 and the maximum residue level of cyromazine in edible animal-derived products is strictly regulated to be 50 ng g −1 .…”