A Simple Fluorometric Determination of Vitamin C.

Mori, Kōichi; Kidawara, Miyuki; Iseki, Mari; Umegaki, Chieko; Kishi, Takeo

doi:10.1248/cpb.46.1474

Cited by 20 publications

(10 citation statements)

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“…For fluorimetric detection, we treated the sample with activated charcoal to oxidize the ascorbic acid to dehydroascorbic acid, then with 1,2-phenylenediamine to obtain a fluorescent derivative of the dehydroascorbic acid, 3-(1,2-dihydroxyethyl)furo(3,4-b)quinoxaline-l-one [3,15]. In preliminary trials to identify the best mobile phase for the fluoimetric-detection method, we found that better resolution was obtained with 7:1 methanol: (0.1% metaphosphoric acid) than with 50:50 acetonitrile: (Milli-Q water) adjusted to pH 2.2 with metaphosphoric acid.…”

Section: Resultsmentioning

confidence: 99%

“…Of the latter, HPLC methods have received particular attention. In contrast, fluorimetric detection (after treatment of the sample to obtain a fluorescent derivative of ascorbic acid) shows good specificity and sensitivity [3,15]. In contrast, fluorimetric detection (after treatment of the sample to obtain a fluorescent derivative of ascorbic acid) shows good specificity and sensitivity [3,15].…”

Section: Introductionmentioning

confidence: 99%

“…Ascorbic acid is also involved in different enzyme systems, in collagen formation, in neurotransmission and in immune responses [2]. In the human diet, ascorbic acid is principally supplied by plant foods, Presented at: 23 rd International Symposium on Chromatography, London, UK, October 1 5, 2000 though it is also present in animal foods including liver [3] and fish [4]. In the human diet, ascorbic acid is principally supplied by plant foods, Presented at: 23 rd International Symposium on Chromatography, London, UK, October 1 5, 2000 though it is also present in animal foods including liver [3] and fish [4].…”

Section: Introductionmentioning

confidence: 99%

“…HPLC methods with UV detection [14] or electrochemical detection [12,16] are considered insufficiently specific, and can often be adversely affected by interfering substances. The different types of column suitable for HPLC determination of ascorbic acid include the widely used C18 columns [3,13,15], ion-exclusion columns [14], Dionex DA-X-8-11 resins [17], and amino-group columns [18]. The different types of column suitable for HPLC determination of ascorbic acid include the widely used C18 columns [3,13,15], ion-exclusion columns [14], Dionex DA-X-8-11 resins [17], and amino-group columns [18].…”

Section: Introductionmentioning

confidence: 99%

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Determination of vitamin C in sea urchin: Comparison of two HPLC methods

2001

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A UV-detection and a fJuorimetric-detection HPLC method were compared for the determination of ascorbic acid levels in sea urchin gonad. The UV detection method (direct determination of ascorbic acid at 245 nm) gave a detection limit of 0.19 I~g mL 1 and reproducibihly (6 assays of a sea urchin gonad sample) of 6.35%. The fluorimetric detection method (oxidation of ascorbic acid to dehydroascorbic acid, treatment with 1,2-phenylenediamine to convert the dehydroascorbic acid to a fluorescent product, and fluorimetric detection of the product) gave a detection limit of 0.082 I~g mL 1 and reproducibihly of 0.61%. The fluorimetric method is thus preferable for determination of vitamin C in sea urchin gonad.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of vitamin C in sea urchin: Comparison of two HPLC methods

2001

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“…For the ascorbic acid determination in natural products, analytical methods such as GC‐MS (24), fluorimetry (25), spectrophotometry (26) and HPLC (27) have been reported. Among these techniques, GC‐MS is considered to be one of the most sensitive techniques for the assessment of ascorbic acid (28).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a sunscreen photoprotective effect by ascorbic acid assessment in human dermis using microdialysis and gas chromatography mass spectrometry

Lévêque

Mac-Mary

Muret

et al. 2005

Experimental Dermatology

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Ultraviolet irradiation causes adverse effects like sunburn, photosensitivity reactions or immunologic suppression. The aim of this study was to evaluate the photo-protective outcome of a sunscreen cream (SPF8) by the determination of erythema indexes and the assessment of ascorbic acid and its metabolites in human dermis. These substances were used as markers of oxidative effect. Eight healthy female subjects were enrolled in this study. Two abdominal areas were exposed to solar simulated irradiation with three minimal erythema dose, one with SPF8 application and the other site without SPF8 application. Two other areas were used as control, one without SPF8 application and the other site after SPF8 application. Ascorbic acid and its metabolites (dehydroascorbic acid, threonic acid, oxalic acid and xylose) were collected from human dermis by microdialysis and assessed by gas chromatography mass spectrometry. Irradiated site without sunscreen application had significantly demonstrated lower dermis ascorbic acid concentrations and a higher erythema index than the three other sites (P < 0.05). Threonic acid, oxalic acid and xylose dermis concentrations were significantly higher in site III than in the control site I (P < 0.05). The protected-irradiated site did not show erythema formation and there was stability of ascorbic acid dermis concentrations with non-variation in its metabolites. The assessment of ascorbic acid and its metabolites in human dermis could be an efficient tool to demonstrate the oxidative process and consequently to control the efficiency of sunscreen creams against undesirable UV effects.

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