Determination of vitamin C in sea urchin: Comparison of two HPLC methods

Abstract

A UV-detection and a fJuorimetric-detection HPLC method were compared for the determination of ascorbic acid levels in sea urchin gonad. The UV detection method (direct determination of ascorbic acid at 245 nm) gave a detection limit of 0.19 I~g mL 1 and reproducibihly (6 assays of a sea urchin gonad sample) of 6.35%. The fluorimetric detection method (oxidation of ascorbic acid to dehydroascorbic acid, treatment with 1,2-phenylenediamine to convert the dehydroascorbic acid to a fluorescent product, and fluorimetric detection of the product) gave a detection limit of 0.082 I~g mL 1 and reproducibihly of 0.61%. The fluorimetric method is thus preferable for determination of vitamin C in sea urchin gonad.

A screening method for the determination of ascorbic acid in fruit juices and soft drinks

“…Different types of HPLC columns, including C 18 (Burini, 2007;Chen & Sato, 1995;de Quirós, López-Hernández, & Simal-Lozano, 2001;Furusawa, 2001;Garrido-Frenich, Hernández-Torres, Belmonte-Vega, Martínez-Vidal, & Plaza-Bolaños, 2005;Nojavana et al, 2008) bonded-phase NH 2 (Rodríguez-Comesaña, García-Falcón, & Simal-Gándara, 2002), polymeric columns (Lopes, Drinkine, Saucier, & Glories, 2006) and diol columns (Tai & Gohda, 2007) have been reported in the literature for the analysis of ascorbic acid in foods. GC-based methods have also been demonstrated to be suitable for determining ascorbic acid in foodstuffs (Silva, 2005).…”