Determination of ascorbic and dehydroascorbic acid in lean and fatty fish species by high-performance liquid chromatography with fluorometric detection

Iglesias, Jacobo; González, M. Jesús; Medina, Isabel

doi:10.1007/s00217-006-0269-1

Cited by 17 publications

(8 citation statements)

References 19 publications

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“…Determination of Ascorbate Concentration. Ascorbate (AscH À ) was acidically extracted from the minced fish muscle, oxidized, derivatized with DMPD, and analyzed by HPLC coupled to a fluorescence detector according to the method of Iglesias et al 30 Extraction and Quantification of Heme Proteins. Heme proteins were extracted from mackerel muscle according to the method of Larsson et al 31 Briefly, samples were homogenized in a 50 mM Tris buffer (pH 8.0) containing 80 mM KCl and 1 mM EDTA.…”

Section: ' Materials and Methodsmentioning

confidence: 99%

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Role of the Raw Composition of Pelagic Fish Muscle on the Development of Lipid Oxidation and Rancidity during Storage

Maestre¹,

Pazos²,

Medina³

2011

J. Agric. Food Chem.

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The muscle composition of a pelagic fish species, Atlantic mackerel (Scomber scombrus), has been studied to determine the relationship with its susceptibility to develop lipid oxidation during chilled storage. For such an aim, the initial concentrations of the major components (water, total lipids, protein, and PUFAs) and minor pro-oxidant and antioxidant components (ascorbic acid, α-tocopherol, hemoglobin, total iron, LMW-iron, copper, and zinc) of different batches of mackerel were characterized. For the study, several batches of mackerel were caught during the spring and summer periods. The different batches were subjected to chilled storage, and the onset of lipid oxidation was statistically related with the initial muscle composition. Results showed significant compositional differences among the mackerel lots, especially for the muscle lipid content (2.83-9.50%). In a first step, a Pearson correlation test was used to check the influence of each component on the progress of lipid oxidation. Results showed a significant relationship between shelf life and water and total lipid contents. Multiple regression was performed to reveal the contribution of each component to the susceptibility to lipid oxidation. The model obtained combines the content of PUFAs, total iron, hemoglobin, and ascorbic acid. An accurate prediction of shelf life in terms of rancidity was achieved by the model created (R(2) = 0.9975). These results establish that the levels of endogenous pro-oxidants and antioxidants present in fish muscle together with the polyunsaturated lipids are relevant factors affecting the shelf life of mackerel muscle.

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Section: ' Materials and Methodsmentioning

confidence: 99%

“…Ascorbate (AscH – ) was acidically extracted from the minced fish muscle, oxidized, derivatized with DMPD, and analyzed by HPLC coupled to a fluorescence detector according to the method of Iglesias et al…”

Section: Methodsmentioning

confidence: 99%

Role of the Raw Composition of Pelagic Fish Muscle on the Development of Lipid Oxidation and Rancidity during Storage

Maestre¹,

Pazos²,

Medina³

2011

J. Agric. Food Chem.

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“…However, there may be some drawbacks in sensitivity, selectivity, stability, or difficulties in sample preparation. Nowadays, high-performance liquid chromatography (HPLC) (Iglesias et al, 2006;Shakya and Navarre, 2006;Lopes et al, 2006;Frenich et al, 2005;Kand'ar et al, 2005;Brause et al, 2003;Yuan and Chen, 1999) and capillary electrophoresis (Law et al, 2005;Wu et al, 2007;Tang, and Wu, 2005;Zinellu et al, 2004;Jin and Jiang, 2002), with various detection methods has been the most used technique for the analysis of ascorbic acid in different samples. The amount of ascorbic acid has been reported in dog rose samples using conventional extraction method (Bozan et al, 1998;Halasova and Jicinska, 1998).…”

Section: Introductionmentioning

confidence: 99%

Extraction and quantitative determination of ascorbic acid during different maturity stages of Rosa canina L. fruit

Nojavan

Khalilian

Kiaie³

et al. 2008

Journal of Food Composition and Analysis

102

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“…Serum or plasma samples were centrifuged at 1,500 g for 15 min at 4 • C and frozen at −80 • C. Plasma VC content was detected by 1,290 Infinity Ultrahigh-Performance Liquid Chromatography (UHPLC) from Agilent Company in Guangzhou KingMed Center for Clinical Laboratory Co., Ltd., Guangdong Province, China. The detection method has been described in detail previously (32). Briefly, the samples were pretreated by protein precipitation, centrifugation, etc.…”

Section: Methodsmentioning

confidence: 99%

Effects of Oral Vitamin C Supplementation on Liver Health and Associated Parameters in Patients With Non-Alcoholic Fatty Liver Disease: A Randomized Clinical Trial

Yang

et al. 2021

Front. Nutr.

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Non-alcoholic fatty liver disease (NAFLD) is now recognized as the most prevalent hepatic disorder worldwide, and an unhealthy lifestyle is the leading risk factor for its occurrence. Vitamin C (VC) has been suggested to protect NAFLD, whereas evidence from randomized controlled trials (RCTs) is sparse. In this study, we aimed to investigate the potential benefits of VC supplementation daily on liver health and associated parameters in patients with NAFLD. In this double-blind, RCT, 84 patients with NAFLD, aged 18–60 years old, were assigned to 12 weeks of oral treatment with either low (250 mg/day, n = 26), medium (1,000 mg/day, n = 30), or high (2,000 mg/day, n = 28) doses of VC supplements. After the intervention, the Medium group had a more significant decrease in aspartate aminotransferase [Medium, −5.00 (−10.25, −1.75) vs. High, −2.50 (−7.75, 0.00), P = 0.02] and alanine aminotransferase [Medium, −8.00 (−18.00, −1.75) vs. High, −3.50 (−13.75, 4.25), P = 0.05; Medium vs. Low, −3.00 (−9.00, 5.50), P = 0.031]. The levels of other indicators of liver health, such as gamma-glutamyl transferase, alkaline phosphatase, total bilirubin, and direct bilirubin were decreased after the intervention but comparable among the three groups and so did the parameters of glucose metabolism, such as fasting insulin, fasting glucose, and homeostasis model assessment for insulin resistance. The plasma level of VC in patients and total adiponectin and high molecular weight (HMW) adiponectin levels were also elevated but not in a dose-dependent manner. Meanwhile, analysis of fecal microbiota composition showed an increase in the alpha diversity (Abundance-based Coverage Estimator (ACE), Shannon, chao1, and Simpson) both in the Low and the Medium groups. A total of 12 weeks of VC supplementation, especially 1,000 mg/day, improved liver health and glucose metabolism in patients with NAFLD. The elevated plasma levels of VC, total and HMW adiponectin, and the improvement of intestinal microbiota may have made some contributions.

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Determination of ascorbic and dehydroascorbic acid in lean and fatty fish species by high-performance liquid chromatography with fluorometric detection

Cited by 17 publications

References 19 publications

Role of the Raw Composition of Pelagic Fish Muscle on the Development of Lipid Oxidation and Rancidity during Storage

Role of the Raw Composition of Pelagic Fish Muscle on the Development of Lipid Oxidation and Rancidity during Storage

Extraction and quantitative determination of ascorbic acid during different maturity stages of Rosa canina L. fruit

Effects of Oral Vitamin C Supplementation on Liver Health and Associated Parameters in Patients With Non-Alcoholic Fatty Liver Disease: A Randomized Clinical Trial

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