A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves

Pinit, Sompop; Chadchawan, Supachitra; Chaiwanon, Juthamas

doi:10.1002/aps3.11395

Cited by 6 publications

(4 citation statements)

References 11 publications

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“…After spectral reflectance measurement (see below), the same leaves were harvested for Pi content determination using the Pi-molybdenum blue assay described previously [ 10 ]. In brief, each leaf was punched using a paper puncher to harvest four 3-mm-diameter leaf discs.…”

Section: Methodsmentioning

confidence: 99%

Hyperspectral and genome-wide association analyses of leaf phosphorus status in local Thai indica rice

et al. 2022

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Phosphorus (P) is an essential mineral nutrient and one of the key factors determining crop productivity. P-deficient plants exhibit visual leaf symptoms, including chlorosis, and alter spectral reflectance properties. In this study, we evaluated leaf inorganic phosphate (Pi) contents, plant growth and reflectance spectra (420–790 nm) of 172 Thai rice landrace varieties grown hydroponically under three different P supplies (overly sufficient, mildly deficient and severely deficient conditions). We reported correlations between Pi contents and reflectance ratios computed from two wavebands in the range of near infrared (720–790 nm) and visible energy (green-yellow and red edge) (r > 0.69) in Pi-deficient leaves. Artificial neural network models were also developed which could classify P deficiency levels with 85.60% accuracy and predict Pi content with R2 of 0.53, as well as highlight important waveband sections. Using 217 reflectance ratio indices to perform genome-wide association study (GWAS) with 113,114 SNPs, we identified 11 loci associated with the spectral reflectance traits, some of which were also associated with the leaf Pi content trait. Hyperspectral measurement offers a promising non-destructive approach to predict plant P status and screen large germplasm for varieties with high P use efficiency.

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Section: Methodsmentioning

confidence: 99%

Hyperspectral and genome-wide association analyses of leaf phosphorus status in local Thai indica rice

et al. 2022

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“…It has been well-established that a strong acid condition is needed to prevent auto-reduction of molybdenum in the absence of phosphate [ 24 ]. Different strong acids, such as sulfuric acid, hydrochloric acid, perchloric acid, and nitric acid, have been used in the molybdenum blue method for phosphate detection [ 40 , 41 , 42 , 43 ]. However, sulfuric acid has been mostly preferred in this method since nitric and perchloric acids (oxidizing acids) interfere with the reduction reaction of phosphomolybdenum acid [ 24 , 44 ], and the chloride ions in the hydrochloric acid hinder the development of the molybdenum blue complex [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

A Colorimetric Dip Strip Assay for Detection of Low Concentrations of Phosphate in Seawater

Heidari-Bafroui

Charbaji

Anagnostopoulos

et al. 2021

Sensors

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Nutrient pollution remains one of the greatest threats to water quality and imposes numerous public health and ecological concerns. Phosphate, the most common form of phosphorus, is one of the key nutrients necessary for plant growth. However, phosphate concentration in water should be carefully monitored for environmental protection requirements. Hence, an easy-to-use, field-deployable, and reliable device is needed to measure phosphate concentrations in the field. In this study, an inexpensive dip strip is developed for the detection of low concentrations of phosphate in water and seawater. In this device, ascorbic acid/antimony reagent was dried on blotting paper, which served as the detection zone, and was followed by a wet chemistry protocol using the molybdenum method. Ammonium molybdate and sulfuric acid were separately stored in liquid form to significantly improve the lifetime of the device and enhance the reproducibility of its performance. The device was tested with deionized water and Sargasso Sea seawater. The limits of detection and quantification for the optimized device using a desktop scanner were 0.134 ppm and 0.472 ppm for phosphate in water and 0.438 ppm and 1.961 ppm in seawater, respectively. The use of the portable infrared lightbox previously developed at our lab improved the limits of detection and quantification by a factor of three and were 0.156 ppm and 0.769 ppm for the Sargasso Sea seawater. The device’s shelf life, storage conditions, and limit of detection are superior to what was previously reported for the paper-based phosphate detection devices.

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“…We speculate that the growth suppression due to AMF inoculation found in this study might be a result of the soil P condition. The available P content of the soil was 37.38 ppm, which is considered a high P level for rice seedlings [47]. This available P concentration is generally found in lowland rice paddies [48,49].…”

Section: Discussionmentioning

confidence: 99%

Acaulospora as the Dominant Arbuscular Mycorrhizal Fungi in Organic Lowland Rice Paddies Improves Phosphorus Availability in Soils

2021

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Flooding in rainfed lowlands greatly impairs the mutualistic relationship between indigenous arbuscular mycorrhizal fungi (AMF) and rice. In flooded soils, root colonization by AMF is arrested, but some AMF genera, defined as the core AMF, remain present. However, the core AMF in rainfed lowlands and their symbiotic roles remain unknown. Here, we showed that Acaulospora fungi were the core AMF in rice seedling roots of the Sangyod Muang Phatthalung (SMP) landrace rice variety grown in non-flooded and flooded paddy soils. Subsequently, indigenous Acaulospora spores were propagated by trap cultures using maize as the host plants. Therefore, to clarify the roles of cultured Acaulospora spores in a symbiotic partnership, the model japonica rice variety Nipponbare was grown in sterile soil inoculated with Acaulospora spores, and recolonized with a native microbial filtrate from the organic rice paddy soil. Our data demonstrated that the inoculation of Acaulospora spores in well-drained soil under a nutrient-sufficient condition for six weeks enabled 70 percent of the rice roots to be colonized by the fungi, leading to higher phosphate (Pi) accumulation in the mycorrhizal roots. Unexpectedly, the growth of rice seedlings was significantly suppressed by inoculation while photosynthetic parameters such as fractions of incoming light energy and relative chlorophyll content were unaltered. In the soil, the Acaulospora fungi increased soil phosphorus (P) availability by enhancing the secretion of acid phosphatase in the mycorrhizal roots. The findings of this work elucidate the symbiotic roles of the dominant Acaulospora fungi from lowland rice paddies.

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A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves

Cited by 6 publications

References 11 publications

Hyperspectral and genome-wide association analyses of leaf phosphorus status in local Thai indica rice

Hyperspectral and genome-wide association analyses of leaf phosphorus status in local Thai indica rice

A Colorimetric Dip Strip Assay for Detection of Low Concentrations of Phosphate in Seawater

Acaulospora as the Dominant Arbuscular Mycorrhizal Fungi in Organic Lowland Rice Paddies Improves Phosphorus Availability in Soils

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